A Gram-positive, yellow-pigmented, actinobacterial strain, DW152 T , was isolated from a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis indicated that strain DW152 T exhibited low similarity with many species with validly published names belonging to the genera Micrococcus and Arthrobacter. However, phenotypic properties including chemotaxonomic markers affiliated strain DW152T to the genus Micrococcus. Strain DW152 T had ai-C 15 : 0 and i-C 15 : 0 as major cellular fatty acids, and MK-8(H 2 ) as the major menaquinone. The cell-wall peptidoglycan of strain DW152 T had L-lysine as the diagnostic amino acid and the type was A4a.The DNA G+C content of strain DW152 T was 68.0 mol%. In 16S rRNA gene sequence analysis, strain DW152 T exhibited significant similarity with Micrococcus terreus NBRC 104258T , but the mean value of DNA-DNA relatedness between these strains was only 42.3 %. Moreover, strain DW152T differed in biochemical and chemotaxonomic characteristics from M. terreus and other species of the genus Micrococcus. Based on the above differences, we conclude that strain DW152 T should be treated as a novel species of the genus Micrococcus, for which the name Micrococcus lactis sp. nov. is proposed. The type strain of Micrococcus lactis sp. nov. is DW152 T (5MTCC10523 T 5DSM 23694 T ).
Total population of cellulose degrading bacteria was studied in a landfill ecosystem as a part of microbial diversity study. Samples were obtained from 3 and 5 feet depth of a local landfill being operated for past 10 years. Among many isolates, 22 bacterial strains were selected based on their capability to decompose carboxymethyl cellulose (CMC). These isolates were cultivated on agar medium with CMC as the carbon source. All isolates were Gram positive, endospore forming and alkalophilic bacteria with optimum growth pH 9-10. They were grouped based on the phenotypic and chemotaxonomic characters and representative strains of different groups along with high carboxymethyl cellulase (CMCase) producing strains were included for further characterization. Analysis of 16S rRNA gene indicated that these strains belong to different species of the genus Bacillus. Maximum CMCase activity of 4.8 U/ml at 50°C was obtained by strain LFC15. Results in the present study indicated the potential of waste land ecosystems such as landfill are potential source for isolation of industrially important microorganisms.
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