Enrichment and optimization of anaerobic bacterial mixed culture for conversion of syngas to ethanol

Singla, Ashish; Verma, Dipti; Lal, Banwari; Sarma, Priyangshu M.

doi:10.1016/j.biortech.2014.08.083

Cited by 38 publications

(27 citation statements)

References 31 publications

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“…Enrichment of acetogens in chicken manure in India [4] and cow manure in China [36] shows the potential of natural inocula. However, Clostridium difficile and C. sordellii, acetogenic human pathogens, were detected in the enriched fermentation [36], which suggests that extreme caution should be exercised in the selection and use of syngas fermenting microorganisms.…”

Section: Species and Habitatmentioning

confidence: 99%

“…However, "these studies have yet to define a methodology for generating high ethanol production levels with stable culture." [4]. In this paper, we present a review of feedstocks, syngas production, metabolic pathway, bioreactor design, mass transfer, thermodynamics, electrochemistry and microbial kinetics of the syngas fermentation process and propose a conceptual model to describe the syngas fermentation.…”

Section: Introduction To Syngas Fermentationmentioning

confidence: 99%

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Syngas Fermentation: A Microbial Conversion Process of Gaseous Substrates to Various Products

2017

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Biomass and other carbonaceous materials can be gasified to produce syngas with high concentrations of CO and H 2 . Feedstock materials include wood, dedicated energy crops, grain wastes, manufacturing or municipal wastes, natural gas, petroleum and chemical wastes, lignin, coal and tires. Syngas fermentation converts CO and H 2 to alcohols and organic acids and uses concepts applicable in fermentation of gas phase substrates. The growth of chemoautotrophic microbes produces a wide range of chemicals from the enzyme platform of native organisms. In this review paper, the Wood-Ljungdahl biochemical pathway used by chemoautotrophs is described including balanced reactions, reaction sites physically located within the cell and cell mechanisms for energy conservation that govern production. Important concepts discussed include gas solubility, mass transfer, thermodynamics of enzyme-catalyzed reactions, electrochemistry and cellular electron carriers and fermentation kinetics. Potential applications of these concepts include acid and alcohol production, hydrogen generation and conversion of methane to liquids or hydrogen.

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Section: Species and Habitatmentioning

confidence: 99%

Section: Introduction To Syngas Fermentationmentioning

confidence: 99%

Syngas Fermentation: A Microbial Conversion Process of Gaseous Substrates to Various Products

2017

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“…Mixed anaerobic microbial consortium used for this study was enriched from chicken faecal material by growing on Pfennig's basal medium and using syngas as sole carbon source (Singla et al, 2014). Pfennig's basal media (PBM) medium containing (per liter) 50 ml mineral stock solution, 10 ml trace metal stock solution, 10 ml vitamin stock solution, 1 g yeast extract, 1 ml resazurin (0.1%) and 20 ml L-cysteine HCl (2.5%) was used for the initial enrichment of microbial strains.…”

Section: Microorganism and Medium Compositionmentioning

confidence: 99%

“…Higher headspace volume allows more gaseous substrate to be available for microbes to utilize. The experimental set-up and the method used for media preparation have been previously described (Singla et al, 2014). The consortium was grown under anaerobic conditions at 150 rpm and 37 C in an orbital shaker.…”

Section: Experimental System and Bioconversion Studies Adopted For Mementioning

confidence: 99%

“…These bacteria are strictly anaerobic and produce acetate as the major fermentation product (Muller, 2003). Homoacetogenic bacteria is capable to produce ethanol from syngas fermentation includes C. autoethanogenum, Mesophilic bacterium P7, Alkalibaculum bacchi, Clostridium ljungdahlii, Clostridium drakei, Butyribacterium methylotrophicum, Clostridium ragsdalei, Eubacterium limosum and mixed consortium TERI SA1 among others Mohammadi et al, 2012;Singla et al, 2014). So, far syngas bio-conversion was mainly studied using pure cultures or defined cocultures (Lundie and Drake, 1984;Hurst and Lewis, 2010;Kundiyana et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Optimization and Molecular Characterization of Syngas Fermenting Anaerobic Mixed Microbial Consortium TERI SA1

Singla

Kumar

Lavania

et al. 2017

Int. J. Renew. Energy Dev.

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The present study focused on the optimization and molecular characterization of anaerobic mixed consortium TERI SA1 that can utilize synthesis gas as sole carbon source for volatile fatty acids production. Optimization study using Box- Behnken design and RSM methodology was carried out in order to investigate the effect of three medium factors on metabolite formation from synthesis gas bioconversion: (yeast extract (0.0–2.0 g/L), ammonium chloride (0.0–1.5 g/L) and corn steep liquor (0.0-10 g/L). Optimized parameters enhanced the production of volatile fatty acids upto 3.9 g/L, which indicated an increase of around 289 % from the non-optimized conditions. Furthermore, two approaches were used for isolation and phylogenetic identification of anaerobic consortium TERI SA1 involving 16S rRNA sequencing of culturable bacterial isolates as well as meta-genomic approach (by making a 16S rRNA gene library of total community DNA). Based on similarity search with NCBI database selected positive clones were most closely related with acetogenic microorganisms Clostridium scatalogenes, Clostridium carboxydivorans, Clostridium drakei and Uncultured Clostridium sp. and strains isolated by culturable method (ASH051 and ASH 052) with Clostridium scatalogenes, and Clostridium drakei. These strains have previously been reported for acetic acid production from syngas bioconversion.Article History: Received July 16th 2017; Received in revised form September 13rd 2017; Accepted Sept 28th 2017; Available onlineHow to Cite This Article: Singla, A., Kumar, S., Lavania, M., Chhipa, H., Kapardar, R., Rastogi, S., Lal, B., and Sarma, P.M. (2017) Optimization and Molecular Characterization of Syngas Fermenting Anaerobic Mixed Microbial Consortium TERI SA1.International Journal of Renewable Energy Development, 6(3), 241-251.https://doi.org/10.14710/ijred.6.3.241-251

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Biomass Processing via Thermochemical–Biological Hybrid Processes

Torri

Rombolà

Kiwan

et al. 2021

Biomass Valorization

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Enrichment and optimization of anaerobic bacterial mixed culture for conversion of syngas to ethanol

Cited by 38 publications

References 31 publications

Syngas Fermentation: A Microbial Conversion Process of Gaseous Substrates to Various Products

Syngas Fermentation: A Microbial Conversion Process of Gaseous Substrates to Various Products

Optimization and Molecular Characterization of Syngas Fermenting Anaerobic Mixed Microbial Consortium TERI SA1

Biomass Processing via Thermochemical–Biological Hybrid Processes

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