Characterization and Phylogenetic Diversity of Carboxymethyl Cellulase Producing Bacillus Species from a Landfill Ecosystem

Abstract

Total population of cellulose degrading bacteria was studied in a landfill ecosystem as a part of microbial diversity study. Samples were obtained from 3 and 5 feet depth of a local landfill being operated for past 10 years. Among many isolates, 22 bacterial strains were selected based on their capability to decompose carboxymethyl cellulose (CMC). These isolates were cultivated on agar medium with CMC as the carbon source. All isolates were Gram positive, endospore forming and alkalophilic bacteria with optimum growth pH 9-10. They were grouped based on the phenotypic and chemotaxonomic characters and representative strains of different groups along with high carboxymethyl cellulase (CMCase) producing strains were included for further characterization. Analysis of 16S rRNA gene indicated that these strains belong to different species of the genus Bacillus. Maximum CMCase activity of 4.8 U/ml at 50°C was obtained by strain LFC15. Results in the present study indicated the potential of waste land ecosystems such as landfill are potential source for isolation of industrially important microorganisms.

Widespread Antibiotic, Biocide, and Metal Resistance in Microbial Communities Inhabiting a Municipal Waste Environment and Anthropogenically Impacted River

“…Microbial activity within landfills drives decomposition of inorganic and organic compounds to stabilize the waste. Other microbial activities observed from landfills include ammonium oxidation ( 3 ), nitrous oxide reduction ( 4 ), and cellulose hydrolysis ( 5 , 6 ). Despite the importance of these processes and other processes for degradation of landfill waste, the microbial lineages colonizing landfills and their associated metabolic potential are still not well understood.…”

Isolation and optimization of the fermentation condition of cellulolytic microbial isolates from cassava waste water

“…Uzochukwu et al (2001) reported that high level of cassava waste water are produced daily and drained onto roads, streets, rivers and agricultural lands in gari producing communities of Nigeria. These singular activities tend to expose the waste water to microbial contamination [14] Though the statement by Korpole [3], that cellulose is the most abundant biological compound on terrestrial and aquatic ecosystem and is the main component of plant biomass could also be attributed to the findings of the current research work. A similar statement from Deke et al [4], that the dominant waste material from agricultural industry in the form of stalks, stems and husk, there has been great interest in utilizing cellulose as an energy resource and feed.…”

“…Cellulose the substrate of cellulase is the most abundant biological compound on terrestrial and aquatic ecosystem, and is the main component of plant biomass [3]. It is the dominant waste material from agricultural industry in the form of stalks, stems and husk, and there has been great interest in utilizing cellulose as an energy resource and feed [4].…”

Microbial Diversity and Succession in the Chinese Luzhou-Flavor Liquor Fermenting Cover Lees as Evaluated by SSU rRNA Profiles

“…The 16S rRNA and 18S rRNA sequences without chimeras were submitted to compare with sequences from GenBank and the percent similarity was then determined. The phylogenetic trees based on the 16S rRNA and 18S rRNA were respectively constructed with the MEGA 5.0 by the neighbor-joining method with the Kimura two-parameter model [7].…”