The use of somatic embryo for in vitro selection program is very useful since the selected traits will be inherited in the progeny. This study was aimed to obtain somaclonal variants for drought tolerances of soybean genotypes from in vitro selection using polyethylene glycol (PEG). The experiments were set up in two factors with completely randomize design and each treatment was replicated ten times. Four soybean genotypes (Tanggamus, Yellow Biloxi, were used in this study as first factor experiment. In vitro selection was in embryogenic callus level using media containing PEG (0, 5, 10, 15, and 20%) (Tanggamus, Yellow Biloxi, digunakan dalam percobaan ini sebagai faktor perlakuan pertama. Seleksi ini vitro dilakukan pada tahap kalus embriogenik seluruh genotipe yang dicoba menggunakan media dengan penambahan PEG (0, 5, 10, 15, and 20% ABSTRAK Penggunaan embrio somatik pada program seleksi in vitro sangat dibutuhkan untuk mendapatkan karakter yang diinginkan serta dapat diturunkan pada generasi berikutnya. Tujuan dari penelitian ini adalah untuk mendapatkan varian somaklon tanaman kedelai yang toleran kekeringan melalui seleksi in vitro dengan agen seleksi PEG (polyethylene glycol). Penelitian ini menggunakan rancangan percobaan acak kelompok dengan dua faktor perlakuan dan masing-masing perlakuan diulang 10 kali. Empat genotipe kedelai

This study aimed to find out the lead tolerance of sengon (Paraserianthes falcataria) seedling based on growth performance, tolerance index, and secretion and accumulation of organic acids content. Seedlings were exposed to lead (Pb) with the concentration of 0, 0.5, 1, 1.5, 5, and 10 mM in liquid nutrient culture for 4 days in order to investigate secretion and accumulation of oxalic, malic, and citric content, and for 15 days to examine growth performance and tolerance index. The result showed that tolerance index and growth performance of sengon seedling were insignificant to the rising of Pb concentration up to 1.5 mM with tolerance index at least 95%, and even caused an increase of fresh weight. However, the tolerance index and growth of sengon decreased significantly due to Pb exposure of 5 and 10 mM. Among the three organic acids, citrate was most dominant as compared to malate and oxalate. Secretion of citrate increased significantly with the rising concentration-1 of Pb 0.5, 1 and 1.5 mM, reaching to 0.464, 0.540, and 0.587 µg m , respectively, or rising according linear line (r = 0.9, p < 0.5). Citrate accumulation showed inconsistent pattern with the rising Pb exposure. The result suggested that sengon seedling have a slightly tolerance to lead by secretion of organic acid especially citric acid.

ABSTRACT<br /><br />The imbalanced supply-demand of soybean is the main reason of soybean import in Indonesia. One of the efforts to increase of soybean production was expanding the planting area to acid soil with a low pH, high content of Aluminum, and low nutrient availability, through giving ameliorant, such as liming organic materials, and mineral fertilization.This study was aimed to obtain the best combination of fertilizer and amelioran for soybean production in acid soil. The study was conducted in experimental station of Tenjo, Bogor from November 2015 to March 2016. The experiment used a randomized complete block design with two factors. The first factor was combinations of fertilizer and amelioran, i.e., P0 (without fertilization); P1 {25 kg urea + 75 kg SP-36 + 50 kg KCl + dolomite ¼ x Aldd (805 kg) ha-1}; P2 {25 kg urea + 75 kg SP-36 + 50 kg KCl + dolomite ¼ x Aldd (805 kg) + 1,250 kg of manure ha-1}; P3 {50 kg urea + 75 kg SP-36 + 50 kg KCl + dolomite ¼ x Aldd (805 kg) + 2,500 kg of manure ha-1}; P4 {75 kg urea + 150 kg SP-36 + 100 kg KCl + dolomite ¼ x Aldd (805 kg) + 2,500 kg of manure ha-1}; P5 {75 kg urea + 150 kg SP-36 + 100 kg KCl + dolomite ½ x Aldd (1,610 kg) ha-1}; P6 {75 kg urea + 150 kg SP-36 + 100 kg KCl + dolomites 1 x Aldd (3,220 kg) ha-1}. The second factor was soybean varieties of Tanggamus and Anjasmoro. The results showed that application of 75 kg urea + 150 kg SP-36 + 100 kg KCl + dolomite ½ x Aldd (1,610 kg) produced the highest productivity (2.91 ton ha-1), but it was not different from reducing the dose of dolomite into ¼ x Aldd (805 kg) with the addition of 2,500  kg of manure.<br /><br />Keywords: Al saturation, chemical properties of soil, nutrient uptake, produktivity, soil amelioran

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