This study was designed to examine the in vitro antimicrobial and antioxidant activities of the methanol, ethanol, water, n-hexane and dicholoromethane extracts of two Allium species (Allium scabriflorum and Allium tchihatschewii) which are endemic for the flora of Turkey. The antimicrobial efficiency of the plant was evaluated according to disc diffusion and microdilution broth methods. The antimicrobial test results showed that the extracts of A. scabriflorum and A. tchihatschewii showed varying degrees of antimicrobial activity on the tested microorganisms. The extracts were screened for their possible antioxidant activities by three complementary tests; DPPH free radical-scavenging, scavenging of hydrogen peroxide and metal chelating activity assays. All the extracts of A. scabriflorum and A. tchihatschewii exhibited lower DPPH free radical scavenging activity but higher metal chelating activity when compared to standards. The values of scavenging of hydrogen peroxide of the extracts were higher than the standards that of α-tocopherol, BHA, BHT and trolox, but close to that of ascorbic acid. In addition to the antioxidant activity of these plants, the total phenolic compounds and flavonoids were also measured in the extracts. The results presented here may suggest that the extracts of A. scabriflorum and A. tchihatschewii possess antimicrobial and antioxidant properties, and therefore, they can be used as a natural preservative ingredient in food and/or pharmaceutical industry.Key words: Allium scabriflorum, Allium tchihatschewii, antimicrobial activity, antioxidant activity, phenolic
IntroductionThe spoilage and poisoning of foods by microorganisms are problems that have not yet been brought under adequate control despite the range of robust preservation techniques available. Raw and/or processed foods are open to contamination during the production, sale and distribution of them (Deak and Beuchat, 1996). Thus, at present, it is a necessity to use the chemical preservatives to prevent the growth of food spoiling microbes in the food industry (Sağdıç and Özcan, 2003). Consumers are increasingly avoiding foods prepared with preservatives of chemical origin, and natural alternatives are therefore needed to achieve sufficiently long shelf life of foods and a high degree of safety with respect to foodborne pathogenic microorganisms (Rauha et al., 2000).Synthetic antioxidants and antimicrobials in use have been shown to have harmful side effects (Gao et al., 1999;Williams et al., 1999;Osawa and Namiki, 1981), therefore, there is a need for more effective, less toxic and cost effective antioxidants and antimicrobials from natural sources. Recently, there are a lot of reports on the antioxidant and antimicrobial activities of plant-based extract, which has a potential application both in the food and in medicinal industries (Zeng et al., 2011).Since ancient times, several species of the genus Allium have been used as foods, spices or herbal remedies. The genus Allium L. is a member of the family Liliaceae a...