Aggregation and Hydrophobicity Properties of 6 Dairy Propionibacteria Strains Isolated from Homemade Turkish Cheeses

Abstract

Autoaggregation, cell surface hydrophobicity, and coaggregation abilities with E. coli of the selected dairy propionibacteria strains could be used as probiotic in foods after in vivo studies.

An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity

“…The bacterial surface charges are also involved in aggregation (Del Re et al 2000;Kos et al 2003;Collado et al 2007;Xu et al 2009). There were positive correlations between the adhesion ability and aggregation to hydrophobicity, as demonstrated by the high adherence or high aggregation to xylene, an apolar solvent (Harty and Knox 1991;Collado et al 2007;Xu et al 2009;Darilmaz et al 2012). In this study, the oral Lactobacillus strains were derived from saliva, and the results were generally consistent with the study of lactobacilli that were isolated from various locations in the mouth (Colloca et al 2000).…”

“…; Darilmaz et al . ). In this study, the oral Lactobacillus strains were derived from saliva, and the results were generally consistent with the study of lactobacilli that were isolated from various locations in the mouth (Colloca et al .…”

“…A certain strain with high auto-and coaggregation might result in a low level of detection in the oral cavity because aggregation followed by swallowing is generally regarded to be an important factor in the removal of organisms from the mouth. In addition, many authors have reported that the aggregation ability is a desirable property for selecting probiotics strains (Collado et al 2007;Keller et al 2011;Darilmaz et al 2012;Tuo et al 2013). Organisms with the ability to coaggregate with other bacteria such as pathogens may have an advantage over non-coaggregating organisms, by aggregating and removing the pathogens from the environment.…”

Effects of hemicellulose-derived saccharides on behavior of Lactobacilli under simulated gastrointestinal conditions

“…Assays were performed as described by Darilmaz, Beyatli, and Yuksekdag (2012) with some modifications: after 24 h of incubation, cells cultured on a given oligosaccharide were washed and suspended in an amount of PBS leading to an OD 620 of about 1.0, and 1.0 mL of n-hexadecane was added to 1.0 mL of cell suspension to form a two-phase system. After a preincubation period of 10 min at 30°C, the solution was mixed by vortexing for 60 s and allowed to stand for 15 min before removing the aqueous phase to measure the OD 620 .…”

Dairy Propionibacteria: Versatile Probiotics

“…Propionibacteria adhesion to intestinal cells leads to exclusion of invasive pathogenic bacteria by competitive adhesion or co-aggregation mechanisms. This concerns pathogens such as Escherichia coli , Pseudomonas aeruginosa , S taphylococcus aureus and Salmonella enterica [41,42,43,44]. Adhesion mechanisms remain poorly understood, but some preliminary experiments point out a role of surface proteins and teichoic acid in adhesion mechanisms [37].…”