The effects of inulin as a prebiotic supplement and the synbiotic interactions of probiotics to improve oxalate degrading activity

Darilmaz, Derya Önal; Sönmez, Şule; Beyatlı, Yavuz

doi:10.1111/ijfs.13912

Cited by 27 publications

(21 citation statements)

References 60 publications

(87 reference statements)

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“…The growth of the strains at 40 • C demonstrates the ability of these microorganisms to grow at extreme temperatures. Bacteria that exhibit this characteristic show higher growth rates and can proliferate in the gastrointestinal tract, where the temperature is higher than 37 • C [43]. Moreover, a high fermentation temperature reduces contamination by other microorganisms [44].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Betancur

Martínez

Téllez-Isaías

et al. 2020

Animals

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Three lactic acid strains were isolated from feces of the native Zungo Pelado breed of pigs (n = 5) and presumably identified as belonging to the Lactobacillaceae family by morphological techniques showing that they were Gram-positive/rod-shaped and catalase- and oxidase-negative. They were then identified by biochemical tests using API 50CHL as Lactobacillus plantarum (CAM6), Lactobacillus brevis (CAM7), and Lactobacillus acidophilus (CL4). However, 16S rRNA identification showed that all three strains were Lactobacillus plantarum. Additionally, all three isolates were able to grow in pH 3 and 4. Interestingly, the growth of the CAM7 strain decreased at pH 5.6 compared to that of the CAM6 strain (p < 0.05), and the growth of the CL4 strain was reduced at pH 7(p < 0.05). All three candidates showed good growth on bile salts (≥0.15%), and CAM6 and CAM7 showed better tolerance at higher concentrations (0.30%). Similarly, all strains tolerated sodium chloride (NaCl) concentrations from 2 to 10%. These strains also grew well at all temperatures tested (30, 37, and 42 °C). The CAM6 strain showed in vitro antibacterial activity against selected enteropathogenic bacteria (Escherichia coli strain NBRC 102203 and Salmonella enterica serovar Typhimurium 4.5.12) and commensal bacteria (Klebsiella pneumoniae ATCC BAA-1705D-5 and Pseudomonas aeruginosa ATCC 15442) and resistance to all antibiotics except amoxicillin. Further studies to evaluate the effects of these probiotic candidate strains in commercial pigs are currently underway.

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Section: Discussionmentioning

confidence: 99%

In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Betancur

Martínez

Téllez-Isaías

et al. 2020

Animals

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“…Prebiotics are fermentable fibers, which include oligosaccharides, that have beneficial effects on intestinal health through the maintenance of mucosal integrity, and most importantly, through the promotion of beneficial bacteria feeding on prebiotics to generate short chain fatty acids [ 15 , 16 ]. For example, inulin used in combination with a probiotic decreased the viability and growth of E. coli [ 17 , 18 ], whereas galacto-oligosaccharides (GOS) was shown to reduce the adhesion of enteropathogenic E. coli to cultured cells [ 19 ]. However, little is known about the direct effect of prebiotics supplementation on genotoxin expression from pks+ E. coli present in the gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

Oligosaccharides increase the genotoxic effect of colibactin produced by pks+ Escherichia coli strains

et al. 2021

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Background Colibactin is a genotoxin that induces DNA double-strand breaks that may lead to carcinogenesis and is produced by Escherichia coli strains harboring the pks island. Human and animal studies have shown that colibactin-producing gut bacteria promote carcinogenesis and enhance the progression of colorectal cancer through cellular senescence and chromosomal abnormalities. In this study, we investigated the impact of prebiotics on the genotoxicity of colibactin-producing E. coli strains Nissle 1917 and NC101. Methods Bacteria were grown in medium supplemented with 20, 30 and 40 mg/mL of prebiotics inulin or galacto-oligosaccharide, and with or without 5 μM, 25 μM and 125 μM of ferrous sulfate. Colibactin expression was assessed by luciferase reporter assay for the clbA gene, essential for colibactin production, in E. coli Nissle 1917 and by RT-PCR in E. coli NC101. The human epithelial colorectal adenocarcinoma cell line, Caco-2, was used to assess colibactin-induced megalocytosis by methylene blue binding assay and genotoxicity by γ-H2AX immunofluorescence analysis. Results Inulin and galacto-oligosaccharide enhanced the expression of clbA in pks+ E. coli. However, the addition of 125 μM of ferrous sulfate inhibited the expression of clbA triggered by oligosaccharides. In the presence of either oligosaccharide, E. coli NC101 increased dysplasia and DNA double-strand breaks in Caco-2 cells compared to untreated cells. Conclusion Our results suggest that, in vitro, prebiotic oligosaccharides exacerbate DNA damage induced by colibactin-producing bacteria. Further studies are necessary to establish whether oligosaccharide supplementation may lead to increased colorectal tumorigenesis in animal models colonized with pks+ E. coli.

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“…Prebiotics are fermentable bers, which include oligosaccharides, that have bene cial effects on intestinal health through the maintenance of mucosal integrity, and most importantly, through the promotion of bene cial bacteria feeding on prebiotics to generate short chain fatty acids (14,15). For example, inulin used in combination with a probiotic decreased the viability and growth of E. coli (16,17), whereas galacto-oligosaccharides (GOS) was shown to reduce the adhesion of enteropathogenic E. coli to cultured cells (18). However, little is known about the direct effect of prebiotics supplementation on genotoxin expression from pks + E. coli present in the gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

Inulin and Galacto-oligosaccharides Increase the Genotoxic Effect of Colibactin Produced by pks+ Escherichia Coli Strains

Oliero¹,

Calvé²,

Fragoso³

et al. 2020

Preprint

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Background: Colibactin is a genotoxin that induces double-strand DNA breaks and is produced by Escherichia coli strains harboring the pks island. Human and animal studies have shown that colibactin-producing gut bacteria promote carcinogenesis and enhance the progression of colorectal cancer through cellular senescence and chromosomal abnormalities. In this study, we investigated the impact of prebiotics on the genotoxicity of colibactin-producing E. coli strains Nissle 1917 and NC101. Methods: Bacteria were grown in medium supplemented with 20, 30 and 40 mg/mL of prebiotics inulin or galacto-oligosaccharide, and with or without 5 µM, 25 µM and 125 µM of iron sulfate. Colibactin expression was assessed by luciferase reporter assay for the clbA gene, essential for colibactin production, in E. coli Nissle 1917 and by RT-PCR in E. coli NC101. The human epithelial colorectal adenocarcinoma cell line, Caco-2, was used to assess colibactin-induced megalocytosis by methylene blue binding assay and genotoxicity by γ-H2AX immunofluorescence analysis. Results: Inulin and galacto-oligosaccharide enhanced the expression of clbA in pks+ E. coli. However, the addition of 125 µM of iron sulfate inhibited the expression of clbA triggered by oligosaccharides. In the presence of either oligosaccharide, E. coli NC101 increased dysplasia and double-strand DNA breaks in Caco-2 cells compared to untreated cells. Conclusion: Our results suggest that, in vitro, prebiotic oligosaccharides exacerbate DNA damage induced by colibactin-producing bacteria. Further studies are necessary to establish whether these results are reproducible in vivo.

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The effects of inulin as a prebiotic supplement and the synbiotic interactions of probiotics to improve oxalate degrading activity

Cited by 27 publications

References 60 publications

In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

In Vitro Characterization of Indigenous Probiotic Strains Isolated from Colombian Creole Pigs

Oligosaccharides increase the genotoxic effect of colibactin produced by pks+ Escherichia coli strains

Inulin and Galacto-oligosaccharides Increase the Genotoxic Effect of Colibactin Produced by pks+ Escherichia Coli Strains

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