Determination of the relationship between oxalate degradation and exopolysaccharide production by different Lactobacillus probiotic strains

Abstract

The aim of this study was to evaluate the protective effects of exopolysaccharide (EPS) production on resistance to gastrointestinal digestive conditions and oxalate by probiotic strains of the species Lactobacillus rhamnosus, Lactobacillus fermentum and Lactobacillus brevis. The oxalate‐degrading ability of the strains was determined using an enzymatic assay. The correlation between oxalate degradation rate and EPS production was not significant (P > 0.05); however, the high‐EPS‐producing L. fermentumIP5 and L. brevisYG7 strains showed high oxalate‐degrading activity, whereas the low‐EPS‐producing strain L. fermentumBP5 demonstrated low oxalate‐degrading activity. The present findings suggest that dietary supplementation with the probiotic strain L. fermentumIP5 could be a promising strategy for the prevention of oxalate stone disease.

The effects of inulin as a prebiotic supplement and the synbiotic interactions of probiotics to improve oxalate degrading activity

“…These strains were selected from 20 Lactobacillus spp. strains as they demonstrated the highest probiotic properties and also consist of different species (Onal Darilmaz et al ., ,b; Sönmez et al ., ).…”

“…The strains were firstly identified based on Gram's stain, morphology and catalase reaction. In addition, these strains were identified with 16S rDNA sequencing with these sequences searched in the GenBank DNA database using Blast function (Sönmez et al ., ). Four strains of Lactobacillus spp.…”

“…Four strains of Lactobacillus spp. among 20 strains were selected as they exhibited the lowest ( L. fermentum BP5) and the highest (strains L. rhamnosus MP1, L. fermentum IP5 and L. brevis YG7) resistance to gastrointestinal conditions, exopolysaccharide (EPS) production, antimicrobial activity, resistance to different antibiotics, cholesterol‐lowering activity, autoaggregation and coaggregation (Onal Darilmaz et al ., ,b; Sönmez et al ., ). Lactobacillus acidophilus NCFM was used as a positive control (Azcarate‐Peril et al ., ) while Lactobacillus delbrueckii DSM 20074 (Turroni et al ., ) was used as the negative control in oxalate degradation experiments.…”

Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

“…More recently, these molecules have been also associated with beneficial roles for health, since they presented anticancer properties (Deepak et al ), cholesterol‐lowering effects (Korcz et al ), immunomodulatory (Hidalgo‐Cantabrana et al ; Ale et al ) and prebiotic (Hamet et al ; Ale et al ) properties. Besides, EPS from LAB have presented protective roles against pathogenic microorganisms (Nagai et al ; Maruo et al ; Ale et al ) and have been related to the prevention of oxalate stone disease (Sönmez et al ), as well as to oxidative damage protection (Chen et al ). Due to these health‐promoting properties, these molecules represent part of a wider group called ‘postbiotics’.…”

Analytical procedures and methods validation for oxalate content estimation

“…The patients who are high risk for kidney stone problems should control oxalate intake to less than 40-50 mg/day (by the American Dietetic Association, 2005). Gut bacterial flora can reduce the oxalate content of digested food through the enzymatic pathway [25][26][27]. Oxalobacter (O.)…”