In the preceding article, a method for measuring total marrow cellularity was described (1). The purpose of this report is to relate numerically (a) the erythroid marrow to the circulating red cell mass, and (b) the granulocytic marrow to the circulating granulocytic leukocytes. Results in three animal species and in man are reported, and are compared with data obtained by other methods.
MATERIALS AND METHODSStudies were performed on Sprague-Dawley rats weighing between 200 to 300 Gm., New Zealand white rabbits weighing 2 to 33/ Kg., and 2 to 3 Kg. male Rhesus monkeys. All animals were given approximately 15 mg. per Kg. of iron as iron dextran2 at least two weeks before the study to insure adequate iron stores. Only those animals with hemoglobin levels above 13 Gm. were used. Reticulocyte counts in rabbits were required to be below 4 per cent and those of monkeys and rats below 3 per cent before the animals were considered suitable for study.Clinical studies were performed on male patients with controlled tuberculosis who were being subjected to thoracotomy. Prior to operation, these patients had normal leukocyte counts and were afebrile, but plasma iron values were usually subnormal. On the morning of operation their red counts were above 4.5 X 106; their reticulocyte counts were less than 2 per cent. They had not been transfused during the previous six months.
I. Erythrocytic seriesA. Studies of the circulating erythrocytes. A red count was performed by enumerating a minimum of 1,000 cells from two or more pipettes in two counting chambers. Reticulocyte counts were performed on dried
Blood refrigerated in acid-citrate-dextrose is known to undergo marked derangement in carbohydrate metabolism and loss of viability during storage. Previous studies of the "storage lesion" have indicated that this metabolic deterioration is arrested once the red cell is introduced into the active circulation of the recipient, and that the ability of the red cells to withstand further in vitro storage is regained (1).The addition of adenosine to stored blood has been shown to produce a repletion of cellular organic phosphates and an increased ability of the cells to oxidize glucose aerobically (2). Further data will be presented in this paper on the reversal of other storage changes and in particular the improved maintenance of the post-transfusion viability of the erythrocyte.
MATERIALS AND METHODS
Blood
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.