The Use of Chromium as a Red‐cell Tag*

Donohue, Dennis M.; Motulsky, Arno G.; Giblett, Eloise R.; Pirzio-Bikoli, G.; Viranuvatti, Vikit; Finch, Clement A.

doi:10.1111/j.1365-2141.1955.tb05508.x

Cited by 156 publications

(39 citation statements)

References 17 publications

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“…Hemoglobin heat stability tests were performed as previously described (4). The red blood cell mass in the propositus was determined using '1Cr-labeled autologous red cells (10). Urine specimens for erythropoietin (ESF)' determination were collected, processed, and assayed in exhypoxic polycythemic mice as previously described (11).…”

Section: Methodsmentioning

confidence: 99%

Hemoglobin Olympia (β20 Valine → Methionine): An Electrophoretically Silent Variant Associated with High Oxygen Affinity and Erythrocytosis

Stamatoyannopoulos¹,

Nute²,

Adamson³

et al. 1973

J. Clin. Invest.

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A B s T R A C T In a family with erythrocytosis, electrophoretic and chromatographic studies failed to demonstrate a hemoglobin variant. However, the oxygen dissociation curves of affected individuals were shifted to the left of normal and this shift persisted when oxygen equilibria were studied in 2,3-diphosphoglycerate-stripped hemolysates. A mutant hemoglobin was evidently present in the red blood cells of the affected persons and was responsible for the increased oxygen affinity and erythrocytosis. Specific staining of tryptic peptidle maps of P-chains from the propositus showed that peptide PT3 was positive for a sulfur-containing amino acid. Amino acid analysis yielded a composition identical to that of normal PT3, except that there were 2.6 residues of valine and 0.4 residues of methionine (normal composition: Val = 3.0, Met = 0). This suggested that the P-chains of affected individuals consisted of a mixture of two kinds of chains, 40% of which had a methionyl residue in PT3. Structural studies of isolated cyanogen bromide fragments demonstrated unequivocally that, in the abnormal P-chains, valine in position 20 is replaced by methionine. The new hemoglobin mutant is designated hemoglobin Olympia (P20 (B2) valine >methion-ine).

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Section: Methodsmentioning

confidence: 99%

Hemoglobin Olympia (β20 Valine → Methionine): An Electrophoretically Silent Variant Associated with High Oxygen Affinity and Erythrocytosis

Stamatoyannopoulos¹,

Nute²,

Adamson³

et al. 1973

J. Clin. Invest.

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“…This could be demonstrated in both hemolysate and intact cell systems. The above evidence, and the fact that red cell rejuvenation involves the reincorporation of inorganic phosphate into the organic phosphate fraction, suggested that a purine * Results with the Cr61 method average 5 to 10 per cent lower than those obtained with the Ashby technique due to the excessive loss of Cr51 during the first 24 hours (10). nucleoside phosphorylase mediated the primary reaction.…”

Section: Discussionmentioning

confidence: 99%

“…Post-transfusion survival of blood stored in ACDI was determined by the differential agglutination technique (Ashby) (9) with slight modifications, and by a double isotope method employing Cr' and P' (10). The blood was collected from Type O-RH negative donors directly into plastic bags (kindly supplied by Cutter Laboratories) containing 75 ml.…”

Section: Methodsmentioning

confidence: 99%

Erythrocyte Preservation. Vii. Acid-Citrate-Dextrose-Inosine (Acdi) as a Preservative for Blood During Storage at 4° C1

Gabrio¹,

Donohue²,

Huennekens³

et al. 1956

J. Clin. Invest.

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Previous investigations on erythrocyte preservation (1-4) have demonstrated a marked effect of purine nucleosides on the metabolism of the red cell throughout storage. Attention has been focused upon the effect of adenosine in the maintenance of glucose metabolism and of high energy phosphate compounds, such as adenosine triphosphate (ATP). As a result, not only are the physical and chemical characteristics of the erythrocyte preserved, but the storage period, during which satisfactory post-transfusion viability is maintained, is extended to approximately twice that observed in acid-citrate-dextrose (ACD) preservative alone. It was postulated that the effect of adenosine was the result of an initial enzymatic conversion to inosine by a deaminase and the subsequent splitting of inosine to yield hypoxanthine and ribose-l-phosphate, mediated by a nucleoside phosphorylase (4, 5). Thus, it appeared that inosine might be the actual substrate for the initial enzymic reaction, and hence the preferred substance for viability studies. In order to evaluate a new preservative, it is desirable to correlate 1) the chemical characteristics of the stored red cells, 2) the ability of the cells to survive after transfusion, and 3) possible toxic effects of the preservative. Studies employing acid-citrate-dextroseinosine (ACDI) as a blood preservative are presented in this paper, as well as further investigations on the mechanism of action of purine nucleosides. METHODSProcedures for the determination of phosphate partition, glucose, and osmotic fragility of erythrocytes have been described elsewhere (3,6).Inosine, adenosine, deoxyadenosine, and adenine were obtained from Schwarz Laboratories; guanine, xanthine,

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“…and A.L.) crit was assumed to be 0.92 times the large vessel hematoand into an antecubital vein, with prompt elevation of crit (8,9). Direct measurement from T-1824 concenthe subject's arm, in the remaining 17.…”

Section: Methodsmentioning

confidence: 99%

“…Red cell volume was jects were breathing 100 per cent oxygen from the Col-measured by the dilution of Cr51-labeled red cells as delins respirometer. Thirteen to 19 mg of Evans blue dye scribed previously (8). In calculating plasma volume was rapidly injected from a calibrated syringe into the from the Cr51 cell determinations, the total body hematomain pulmonary artery in 3 patients (D.G., G.S.…”

Section: Methodsmentioning

confidence: 99%