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Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>methylparaben>sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors.

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The human hair follicle, a mini-organ formed with neuroectodermal-mesodermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound Trichotech TM , a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. Trichotech TM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. Trichotech TM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after Trichotech TM treatment (1.0% and 2.0%) on the fibroblast cell line.

The aim of this study was to investigate the cytotoxic activity of the Coriandrum sativum (C. sativum) ethanolic extract (CSEE) in neuroblastoma cells, chemically characterize the compounds present in the CSEE, and predict the molecular interactions and properties of ADME. Thus, after obtaining the CSEE and performing its chemical characterization through dereplication methods using UPLC/DAD-ESI/HRMS/MS, PM6 methods and the SwissADME drug design platform were used in order to predict molecular interactions and ADME properties. The CSEE was tested for 24 h in neuroblastoma cells to the establishment of the IC50 dose. Then, the cell death was evaluated, using annexin-PI, as well as the activity of the effector caspase 3, and the protein and mRNA levels of Bax and Bcl-2 were analyzed by ELISA and RT-PCR, respectively. By UHPLC/DAD/HRMS-MS/MS analysis, the CSEE showed a high content of isocoumarins-dihydrocoriandrin, coriandrin, and coriandrones A and B, as well as nitrogenated compounds (adenine, adenosine, and tryptophan). Flavonoids (apigenin, hyperoside, and rutin), phospholipids (PAF C-16 and LysoPC (16:0)), and acylglicerol were also identified in lower amount as important compounds with antioxidant activity. The in silico approach results showed that the compounds 1 to 6, which are found mostly in the C. sativum extract, obey the “Five Rules” of Lipinski, suggesting a good pharmacokinetic activity of these compounds when administered orally. The IC50 dose of CSEE (20 µg/mL) inhibited cell proliferation and promoted cell death by the accumulation of cleaved caspase-3 and the externalization of phosphatidylserine. Furthermore, CSEE decreased Bcl-2 and increased Bax, both protein and mRNA levels, suggesting an apoptotic mechanism. CSEE presents cytotoxic effects, promoting cell death. In addition to the promising results predicted through the in silico approach for all compounds, the compound 6 showed the best results in relation to stability due to its GAP value.

A técnica percutânea transradial vem sendo utilizada como uma estratégia segura e adequada para intervenções coronárias, não isenta de complicações vasculares. Evidenciar as complicações do procedimento coronário percutâneo via artéria radial. Revisão integrativa da literatura, nas bases de dados SciELO e PubMed de estudos publicados entre 2014 e 2020, disponíveis na íntegra nas línguas portuguesa e inglesa. Sangramento, hematoma e oclusão da artéria radial são as principais complicações do procedimento invasivo, objeto deste estudo. O exercício do enfermeiro contempla a identificação precoce das complicações após os procedimentos percutâneos e agilidade para intervir com o propósito de assegurar a eficiência desse tratamento e garantir a recuperação da saúde do cliente. Entretanto, deve-se ter cuidado com a realização das intervenções de enfermagem padronizadas com o intuito de não as tornar mecânicas e limitadas, logo, insatisfatória para solucionar a complexidade e a particularidade de cada paciente.Descritores: Artéria Radial, Cuidados de Enfermagem, Enfermagem, Intervenção Coronária Percutânea. Transradial procedures and their complications: a view from nursingAbstract: The percutaneous transradial technique has been used as a safe and adequate strategy for coronary interventions, not exempt from vascular complications. To highlight the complications of the percutaneous coronary procedure via the radial artery. Integrative literature review, in the SciELO and PubMed databases of studies published between 2014 and 2020, available in full in Portuguese and English. Result: Bleeding, hematoma and occlusion of the radial artery are the main complications of the invasive procedure, the object of this study. The nurse's exercise includes the early identification of complications after percutaneous procedures and agility to intervene in order to ensure the efficiency of this treatment and ensure the recovery of the client's health. However, care must be taken with the performance of standardized nursing interventions in order not to make them mechanical and limited, therefore, unsatisfactory to solve the complexity and particularity of each patient.Descriptors: Radial Artery, Nursing Care, Nursing, Percutaneous Coronary Intervention. Procedimientos transradiales y sus complicaciones: una mirada de la enfermeríaResumen: La técnica percutánea transradial se ha utilizado como una estrategia segura y adecuada para intervenciones coronarias, no exenta de complicaciones vasculares. Destacar las complicaciones del procedimiento coronario percutáneo a través de la arteria radial. Revisión integradora de la literatura, en las bases de datos SciELO y PubMed de los estudios publicados entre 2014 y 2020, disponibles íntegramente en portugués e inglés. Sangrado, hematoma y oclusión de la arteria radial son las principales complicaciones del procedimiento invasivo, objeto de este estudio. El ejercicio del enfermero incluye la identificación precoz de las complicaciones posteriores a los procedimientos percutáneos y la agilidad para intervenir con el fin de asegurar la eficacia de este tratamiento y asegurar la recuperación de la salud del cliente. Sin embargo, se debe tener cuidado con la realización de intervenciones de enfermería estandarizadas con el fin de no hacerlas mecánicas y limitadas, por lo tanto, insatisfactorias para resolver la complejidad y particularidad de cada paciente.Descriptores: Arteria Radial, Cuidado de Enfermeira, Enfermería, Intervención Coronaria Percutanea.