Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

Oliveira, Carlos Rocha; Spindola, Daniel Gonsales; Garcia, Daniel M.; Erustes, Adolfo Garcia; Bechara, Alexandre Henrique Silveira; Palmeira-dos-Santos, Caroline; Pereira, Gustavo José da Silva; Hinsberger, Andre; Viriato, Ezequiel Paulo; Marcucci, María Cristina; Sawaya, Alexandra Christinie Helena Frankland; Tomaz, Samantha L.; Rodrigues, Elaine G.; Bincoletto, Cláudia

doi:10.1016/j.joim.2019.02.001

Cited by 24 publications

(15 citation statements)

References 28 publications

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“…Authors have suggested the role of angelicin in the tumor inhibitory effects of the extract. (Oliveira et al, 2019;Sigurdsson et al, 2005) It has been reported that administration of root extract consecutively for 7 days exerts a protective effect against technetium irradiated rat liver.…”

Section: Potential Of Angelica Archangelica In Modulating Inflammatory Pathwaysmentioning

confidence: 99%

Understanding the phytochemistry and molecular insights to the pharmacology of Angelica archangelica L. (garden angelica) and its bioactive components

Kaur

Bhatti

2021

Phytotherapy Research

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Plant-derived molecules have enduring usefulness in treating diseases, and herbal drugs have emerged as a vital component of global therapeutic demand. Angelica archangelica L. (A. archangelica), commonly known as garden angelica, is an aromatic food plant used in culinary procedures as a flavoring agent. In the traditional medicine system, it is regarded as an "Angel plant" due to its miraculous curative power. This review aims to provide a comprehensive summary of the plant's taxonomic profile, ethnopharmacology, Phytochemistry, and pharmacological activities. Various in vivo and in vitro experiments have validated that the plant possesses broad pharmacological potential. The biological activities attributed to the plant include anti-anxiety activity, anti-convulsant activity, cognition enhancer, antiviral activity, cholinesterase inhibitory potential, antiinflammatory activity, gastroprotective activity, and radioprotective activity. The beneficial effects of the plant are credited to its bioactive components, that is, coumarins and volatile oils. The review summarizes the pharmacological activities of crude extract and its bioactive fractions and has also explored their target-oriented effects. This review will be of value in undertaking further investigations on the plant with regard to exploring mechanism-based pharmacological approaches on A. archangelica.

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Section: Potential Of Angelica Archangelica In Modulating Inflammatory Pathwaysmentioning

confidence: 99%

Understanding the phytochemistry and molecular insights to the pharmacology of Angelica archangelica L. (garden angelica) and its bioactive components

Kaur

Bhatti

2021

Phytotherapy Research

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“…With the rising cost of health care and the introduction of new targeted therapies, biomarkers have been used as a means of assisting breast cancer diagnosis, prognosis, response prediction, and disease surveillance during and after treatment. Prognostic and therapeutic biomarkers, their utility in breast cancer management, and current recommendations on their clinical use should be paid attention [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

miR-641 Inhibited Cell Proliferation and Induced Apoptosis by Targeting NUCKS1/PI3K/AKT Signaling Pathway in Breast Cancer

Zhang

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. Studies revealed an important role of microRNAs (miRNAs) in multiple cancers, including breast cancer. In the present study, we evaluated the role and function of miR-641 in breast cancer. Methods. The expression level of miR-641 in breast cancer cell lines (Hs-578T, MCF7, HCC1937, and MAD-MB-231) was determined by real-time PCR. Functional analyses, including CCK-8 assay, transwell assay, wound-healing assay, and apoptosis detection, were carried out to explore the roles of miRNA-641 in malignant behaviors of breast cancer. Luciferase report assay was used to investigate the regulatory association of miRNA-641 with its potential targets. Results. The expression levels of miR-641 were downregulated, while the expression levels of nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1) were increased in breast cancer cell lines. The in vitro results showed that miR-641 repressed proliferation and migration/invasion and promoted apoptosis of breast cancer cells. NUCKS1, a positive regulator of phosphatidylinositol-3-kinases (PI3K)/protein-serine-threonine kinase (AKT) pathway, was confirmed as a direct target of miR-641. The of treatment of the PI3K agonist, 740Y-P, could abrogate the antioncogenic potentials of miR-641 in breast cancer cells. Conclusion. miR-641 functioned as a tumor suppressor through the PI3K/AKT signaling pathway via targeting NUCKS1 in breast cancer.

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“…It is composed of Radix Codonopsis, Astragalus membranaceus, Angelica Sinensis, Radix Bupleuri, Rhizoma Chuanxiong, raw Radix Paeoniae Alba, Fructus Forsythiae, Radix Glycyrrhizae, Trichosanthes kirilowii Maxim and Pericarpium Citri Reticulatae Viride. Pharmacological studies have showed that the Radix Codonopsis, Astragalus membranaceus and Angelica Sinensis in RYF have effective therapeutic effect in anti-in ammatory, antitumor, immunity enhancement and so on [6][7][8][9][10]. Meanwhile, the extract of Radix Codonopsis [11], Astragalus membranaceus [12,13], Angelica Sinensis [14], raw Radix Paeoniae Alba [15], Fructus Forsythiae [16] and Trichosanthes kirilowii Maxim [17] could inhibit EMT (epithelial-mesenchymal transition), which is an important development process of cell adhesion and migration.…”

Section: Introductionmentioning

confidence: 99%

Ruyong Formula (RYF) Attenuates the Abnormal Phenotypic Changes of Thymic Epithelial Cells in 4T1 Breast Cancer Mice

He¹,

Shi²,

Luo³

et al. 2021

Preprint

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BackgroundEpithelialization of the breast epithelial cell is the critical step in breast cancer, but the phenotypic changes of thymus epithelial cells (TEC) and the following immune abnormalities during the development of breast cancer are rarely examined. Ruyong Formula (RYF) has been used for thousands of years, our previous researches have shown it could attenuates atrophied thymic epithelial tissue in breast cancer mice, but the mechanism is still unknown.MethodsHPLC was used to analyze the chemical components of RYF. The 4T1 breast cancer mice model was established to study the anticancer effects of RYF. The efficacy of RYF on tumor volume, anti-tumor rate and organ index were observed. The thymus tissue were stained with Hematoxylin-Eosin (H&E) to observe the morphological changes. Cell phenotype marker, such as E-cadherin, α-tubulin and Vimentin were observed by immunofluorescence staining in TGF-β1-induced iTECs after RYF treatment. The mRNA levels of phenotypic markers and phenotype-related transcription factors, including E-cadherin, Vimentin, Zeb-1, Snail 1 and Smad 2 were detected by qPCR. The effect of RYF on the activation of Smad pathway in TGF-β1 induced iTECs was detected by luciferase reporter assay.ResultsRYF could reduce the metastatic rate and the number of pulmonary metastases in breast cancer mice and increased anti-tumor rates. Compared with the thymus in normal group, RYF increased the number of thymocytes in the cortex regions. In vitro study indicated the EMT promotion effect of TGF-β1, shown as the decreasing of E-cadherin and up-regulation of the Vimentin’s expression. The level of Snail 1 and Zeb 1 increased significantly, and the mRNA levels of Smad 2 was up-regulated. Compared with TGF-β1 group, RYF-treated TECs reversed the proteins expression of E-cadherin and Vimentin and the mRNA levels of Snail 1 and Zeb 1. RYF also promoted the proliferation of iTECs, and confronted the TGF-β1 induced phenotypic transition in iTECs.ConclusionThe abnormal thymic function of breast cancer mice was mainly due to the disorder of cortex and medulla regions cells and the atrophy of cortex. Interestingly, RYF could reverse the phenotypic changes of TECs in breast cancer by inhibiting the TGF-β1/Smad pathway.

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

Cited by 24 publications

References 28 publications

Understanding the phytochemistry and molecular insights to the pharmacology of Angelica archangelica L. (garden angelica) and its bioactive components

Understanding the phytochemistry and molecular insights to the pharmacology of Angelica archangelica L. (garden angelica) and its bioactive components

miR-641 Inhibited Cell Proliferation and Induced Apoptosis by Targeting NUCKS1/PI3K/AKT Signaling Pathway in Breast Cancer

Ruyong Formula (RYF) Attenuates the Abnormal Phenotypic Changes of Thymic Epithelial Cells in 4T1 Breast Cancer Mice

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