PurposeThe development of inflammatory periodontal disease in young people is an urgent problem of today's periodontology, and requires a development of new methods that would give an opportunity not only to diagnose but also for prognosis of periodontitis course in a given patients contingent.ResultsCellular structure of periodontal pockets is presented by hematogenous and epithelial cells. Our results are confirmed by previous studies, and show that the penetration of periodontal pathogens leads to formation in periodontal tissue of a highly active complex compounds—cytokines that are able to modify the activity of neutrophils and reduce their specific antibacterial properties. Cytokines not only adversely affect the periodontal tissues, but also cause further activation of cells that synthesized them, and inhibit tissue repair and process of resynthesis of connective tissue by fibroblasts.ConclusionNeutrophilic granulocytes present in each of the types of smear types, but their functional status and quantitative composition is different. The results of our cytological study confirmed the results of immunohistochemical studies, and show that in generalized periodontitis, an inflammatory cellular elements with disorganized epithelial cells and connective tissue of the gums and periodontium, and bacteria form specific types of infiltration in periodontal tissues.
The aim: To establish the features of the structural organization of enamel in various anatomical areas of the tooth and determine their influence on the characteristics of the course of biomineralization processes. Materials and methods: The study of the structural features of enamel and dentin was performed on thin sections of various groups of teeth. Then morphological, histochemical and electron microscopic examination methods were used. Results: The study found that there are three structural and functional barriers to biomineralization of enamel, which are located in different anatomical areas of the tooth crown. Each of them has both general and specific features. Enamel biomineralization is a continuous process of exchange of calcium ions, donor of which is brushite. The stepwise process of biomineralization turns the latter into calcium octaphosphate, which then turns into hydroxyapatite. The latter, when destroyed by carbon dioxide, forms carboxyapatite. Conclusions: In the result of conducted study was established peculiarities of enamel mineralization processes in different anatomical parts of tooth.
Supporting teeth preservation at the restoration of dentition defects with the help of fixed porcelain fused metal dentures is considered one of the most important issues of prosthodontics. Margin line preparation strategies are still disputable nowadays. In most cases preparation is confined to the ledge creation. Its function is to provide a smooth transition of orthopedic restoration to a tooth root and prevent the traumatization of marginal paradontium. These issues are gaining a momentum when the possibility of manufacturing fixed prosthesis with the use of partially or completely ruined teeth with the preserved root as a support appears. Since the opportunities of thermal method for gingiva retraction and marginal line formation around the supporting teeth are not studied enough in modern scientific sources, our research aims to define the mastication efficiency state in patients of sample groups after the fixation of dentures on the supporting teeth prepared for prosthesis with the help of diathermocoagulation and mechanical retraction. The authors of the article have determined the mastication efficiency in the patients with the restored tooth crown. Altogether 54 patients of two sample groups aged from 20 to 60 years and above with completely ruined tooth crowns were examined. Their soft tissues around the supporting teeth were prepared for the fixed prosthesis manufacturing. The first sample group involved 29 patients that made up 53.7% of general studied population. Their supporting teeth gingiva margins were retracted with the help of diathermocoagulation while preparing them for the fixed dentures. While the gingiva margins of the patients of the second sample group, consisting of 25 people (46.3% of general studied population), were retracted with the help of cotton Ultrapak cord, produced by Ultradent (the USA). It is offered in several size options. Mastication efficiency state after prosthesis was determined with the help of mastication test (Ukrainian Utility Patent # 94841). In this case, mastication index was the main diagnostic tool. It was received with the help of computer analysis of digital samples in software “Adobe Photoshop Extended”. The analysis of the dynamics of mastication test indices of the patients of sample group 1 delivered typical results. In a week after the fixation of fixed porcelain fused metal dentures, the reduction of the mean value of mastication test index by 0.2 was fixed. The verification of these changes according to the statistical significance showed positive result (at p = 0.0004). At the same time, the verification of the changes, occurred during the period from the 7th to the 30th day after the fixation of dentures in patients of sample group 1, with the help of Wilcoxon test showed that at p = 0.9 the reduction of the mean value to 5.148 had no statistical significance. While the dynamics of mastication test changes in sample group 2 looked as follows. The reduction of the mean values of mastication test index from 5.184 to 5.112 was observed. So, the spread between the values received before the retraction and in seven days after crown fixation made up 0.072. The conducted statistical verification of these changes (at p = 0.01) proved their statistical significance. In contrast, the collation of the indices received after seven days of denture fixation and in 30-days period after it (from 5.112 to 5.132) at p = 0.961 demonstrated changes at the level of statistical error. According to the received research results, prosthesis with the fixed porcelain fused metal dentures promotes patients’ mastication efficiency restoration. That is traced in the reduction of the mastication test index values received after the 7th day of crown fixation in both sample groups. It should be noted here, that statistically significant difference between the indices of the patients of sample group 1 and 2 was not found. On the 30th day of denture fixation, mastication efficiency indices remained almost the same.
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