A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, wich is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.

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