Properties of sonicated vesicles of three synthetic phospholipids

Roseman, M.; Lentz, Barry R.; Sears, Barry; Gibbes, D.C.; Thompson, T. E.

doi:10.1016/0009-3084(78)90068-3

Cited by 42 publications

(19 citation statements)

References 19 publications

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“…This indeed depends on the indicator as well as on the composition of the liposomes. For example, ions that interact with the phospholipid headgroups such as [Fe(CN)6]'-may lead to an overestimation of the trapped volume (Roseman et al, 1978). The use of purified 6-carboxyfluorescein (6CF) (Ralston et al, 1981) to monitor the volume trapped within DPPC vesicles gave results very similar to those obtained when radiolabeled glucose was used as an indicator .…”

Section: Trapped Volume and Osmotic Activitysupporting

confidence: 48%

Liposomes: Preparation, Characterization, and Preservation

Lichtenberg

Barenholz

1988

Methods of Biochemical Analysis

240

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Section: Trapped Volume and Osmotic Activitysupporting

confidence: 48%

Liposomes: Preparation, Characterization, and Preservation

Lichtenberg

Barenholz

1988

Methods of Biochemical Analysis

240

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“…Among them, POPC is the most abundant [35][36][37] and has been extensively used as a model compound for representation of natural PC mixtures [38]. While the MHF method has been used in synthesis of liposomes with other PC lipids [25][26][27]31], these studies have used molecules with two identical saturated fatty acid chains attached to the glycerol backbone, whereas lipid molecules of natural membranes usually contain two different fatty acids [39]. Fatty acid chains of the POPC molecule are different in length with one of them also containing an unsaturated C=C bond which affects fluidity and other physical properties of the lipid bilayer.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic hydrodynamic focusing based synthesis of POPC liposomes for model biological systems

Mijajlovic

Wright

Živković

et al. 2013

Colloids and Surfaces B: Biointerfaces

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Elsevier's AAM Policy: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution.Elsevier believes that individual authors should be able to distribute their AAMs for their personal voluntary needs and interests, e.g. posting to their websites or their institution's repository, e-mailing to colleagues. However, our policies differ regarding the systematic aggregation or distribution of AAMs to ensure the sustainability of the journals to which AAMs are submitted. Therefore, deposit in, or posting to, subjectoriented or centralized repositories (such as PubMed Central), or institutional repositories with systematic posting mandates is permitted only under specific agreements between Elsevier and the repository, agency or institution, and only consistent with the publisher's policies concerning such repositories. AbstractLipid vesicles have received significant attention in areas ranging from pharmaceutical and biomedical engineering to novel materials and nanotechnology. Microfluidic-based synthesis of liposomes offers a number of advantages over the more traditional synthesis methods such as extrusion and sonication. One such microfluidic approach is microfluidic hydrodynamic focusing (MHF), which has been used to synthesize nanoparticles and vesicles of various lipids. We show here that this method can be utilized in synthesis of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles with controllable size. Since POPC is among the primary constituents of cellular membranes, this work is of direct applicability to modelling of biological systems and development of nanocontainers with higher biologic compatibility for pharmaceutical and medical applications.

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“…Figure 8 shows the exchange of (108, 112) mutant b 5 in these two situations. Both curves were fitted by a single exponential and the upper curve gave a value of kd~, = 0.103+0.004 S -z and the lower curve kay, = 0.097+0.002 s-L For the POPC k = 41 s -z and c, = 10-312500 mM (to allow for the number of lipids per POPC vesicle [15]), then k,, = 1 • l0 s M -t s -z and the overall partition coefficient (k=lkal,) is 1 • 109.…”

Section: Resultsmentioning

confidence: 99%

“…Values used for D, and R, are 1.9 • 10 -7 cm 2 s -t and 106/~ [15] and Dp and Rp are 8 X 10 -~ cm 2 s -1 and 26/~ [16], hence k= u = 9.9 • 109 M -~ s -1. This produces a ratio of kJkoou " 0.01, which is considerably smaller than that seen by Schwarz et al with alamethicin (0.16) [13].…”

Section: K~ou = 4"trn(dp + D)(rp + R)/1000mentioning

confidence: 99%

“…If the lipid packing is similar in the two types of lipid, then the BRPC vesicles will contain 1.9-fold the number of lipid molecules that are in the POPC vesicles. A more precise analysis with a similar bromolipid gave a ratio of 1.7 [15]. Hence, in the stopped-flow experiments the concentration of vesicles in the BRPC experiments was only 50% of the vesicle concentration used in the POPC experiments.…”

Section: K~ou = 4"trn(dp + D)(rp + R)/1000mentioning

confidence: 99%

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Stopped-flow fluorescence studies of the interaction of a mutant form of cytochrome b5 with lipid vesicles

et al. 1994

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Cytochrome b5 binds spontaneously to lipid vescles and also self-associates in aqueous solution. Two mutant proteins have been generated, one has a self-association constant which is less than that of the native protein, while the other has a larger self-association constant. All three proteins have Trp in the membrane-binding domain but as aqueous solutions of these proteins contain differing amounts of monomeric protein, the kinetics of fluorescence enhancement, when the proteins are mixed with lipid vesicles, are complex. Similar complex kinetics are seen when the Trp are quenched by the addition of bromolipid vesicles. The mutant which has Trp 108 and 112 both replaced by Leu does not self-associate and shows monoexponential stopped-flow fluorescence kinetics. Identical rate constants are seen with this mutant for fluorescence enhancement by POPC and fluorescence quenching by three bromolipids with bromines at the 6,7-, 9,10-, and 11,12-positions of thesn-2 acyl chain. This rate constant is only 1% of the calculated collisional rate constant and it is suggested that the reduced rate is caused by a reduction in the number of productive collisions rather than by a slow rate of penetration of the membrane-binding domain into the bilayer.

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Properties of sonicated vesicles of three synthetic phospholipids

Cited by 42 publications

References 19 publications

Liposomes: Preparation, Characterization, and Preservation

Liposomes: Preparation, Characterization, and Preservation

Microfluidic hydrodynamic focusing based synthesis of POPC liposomes for model biological systems

Stopped-flow fluorescence studies of the interaction of a mutant form of cytochrome b5 with lipid vesicles

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