Chlorella and Spirulina are the two of the most well-known microalgae genus. Both microalgae genus have a significant content of proteins, vitamins, pigments, fatty acids, sterols, among others, which make their production/application by the food industry quite interesting. Chlorella genus is a eukaryotic microorganism, whereas Spirulina genus (cyanobacteria) is a prokaryotic microorganism. The aim of this review was to provide an overview on Chlorella and Spirulina microalgae, particularly as an alternative source of functional foods, nutraceuticals, and food supplements, in which the following compound groups were addressed: (I) Long-Chain Polyunsaturated Fatty Acids; (II) Phenolic Compounds; (III) Volatile Compounds; (IV) Sterols; (V) Proteins, Amino Acids, Peptides; (VI) Vitamins; (VII) Polysaccharides; (VIII) Pigments and (IX) Food. Chlorella and Spirulina microalgae and their derivatives are concluded not to be widely commercially exploited. However, they are remarkable sources of functional foods, nutraceuticals and food supplements.
P. tsukubaensis is a yeast-like microorganism that synthesized the biosurfactant mannosylerythritol lipids-B (MEL-B). Production cost can be one of the drawbacks of biosurfactants production. Therefore the development of efficient and cost effective purification strategies and the use of by-products in the culture medium could serve as important strategies to reduce overall process cost. The aim of this work was to evaluate the production of MEL using cassava wastewater, a hydrophilic medium composed of a low-cost substrate which is a by-product of cassava processing, followed by foam fractionation and ultrafiltration of MEL. Cassava wastewater proved to be a feasible culture medium for P. tsukubaensis and MEL-B production as the yield (1.26 g L) was similar to that reported by others using water-soluble carbon sources (up to 2 g/L). Interestingly ultrafiltration with 100 KDa MWCO memabranes (using 20 mL centrifugal devices) led to the purification of MEL-B in one step since ≈ 80% of MEL was recovered, while more than 95% of proteins were found in the permeate. The scale up of the ultrafiltration (up to 500 mL) using a cross flow filtration unit led to very similar results. Overall the ultrafiltration led to a threefold increase in MEL purity in terms of protein (at both scales). The chemical characterisation by NMR confirmed the production of MEL-B homologue and also the production of a second stereoisomer ≈ 9%, while the CG-MS and MALDI-TOFMS analysis confirmed the main fatty acids within the structure of MEL-B (C8:0 and 12:0 and C8:0 and C14:1). Therefore, the process developed here was found to be a good alterntative to the conventional production of MEL which uses synthetic culture medium, solvent extraction (ethyl acetate) and column chromatography (silica) for its purification.
Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.