Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

Abstract

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.

Effects ofCitrus junosby-products fermented with multistrain probiotics on growth performance, immunity, caecal microbiology and meat oxidative stability in broilers

“…Improved growth performance obtained in this study by feeding CJP might be attributed to the improved nutritive value of fermented products together with higher production of secondary metabolites by multispecies probiotic [lactic acid by Lactobacillus spp. (Heres et al, 2003), amylase and protease by B. subtilis (Barros et al, 2013) and antimicrobial substances (bacteriocin) produced by B. subtilis (Zheng et al, 1999)] that improves the nutrient digestibility and intestinal microbial equilibrium of broilers. Dietary supplementation of CJP improved the immune status of broiler by increasing the concentration of serum IgM.…”

Biodegradation of Pharmaceutical Wastes in Treated Sewage Effluents by Bacillus subtilis 1556WTNC

“…The production of β-lactamase in treated sewage effluent medium has not been performed before. However, a study of the production of amylase, protease and lipase in cassava wastewater by Bacillus subtilis LB5a and LB1a strains, conducted by Barros et al (2013), found that both strains produce detectable amounts of the enzymes in comparison to the synthetic liquid medium. In the present study, ten bacterial strains, B. subtilis 1556WTNC (+ve), B. subtilis 1612WTNC (+ ve), B. subtilis 2012WTNC (+ve), B. sterothermophilus 1050WTNC (+ve), B. subtilis 212WTNC (+ve), C. luteola1113WTNC (−ve), C. luteola 313WTNC (−ve), C. luteola 613WTNC (−ve), B. cepacia 103WTNC (−ve), P. fluoresens 1353WTNC (−ve) produced high amount of β-lactamase concentrations in ECY medium.…”

Produção de lipase utilizando manipueira como fonte alternativa de carbono

“…O preparo do substrato seguiu a orientação de Barros et al [9], onde a manipueira foi aquecida até à ebulição, resfriada, centrifugada a 3400 rpm durante 20 min. As Figuras 1 e 2 apresentam a manipueira em sua forma bruta e tratada, respectivamente.…”

“…De acordo com Barros et al [9], a atividade lipolítica é geralmente determinada pela liberação dos ácidos graxos produzidos pela hidrólise, que podem ser quantificados por métodos colorimétricos, fluorimétricos, titulométricos, turbidimétricos.…”