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The aim of this study is to investigate the presence of several interleukins in saliva and how their concentrations may be modified after physical stress. The effects of one kind of somatic stress (sauna heat) on the concentrations of salivary cytokines were studied using ELISA assay. In addition, saliva cortisol was assayed. Fourteen volunteers participated in this study. Each subject attended one control and one stress session which took place at the same hour on different days. Saliva was collected on the control day immediately after a 15‐min rest period in a seated position (R1), and again after an additional 30‐min seated period (R2). On the test day, saliva was collected after 15 min of sitting (S1) and 30 min later (S2) following exposure to the heat of a sauna (90°C, 10 min, 40 per cent humidity). We found that saliva contains the multifunctional polypeptide cytokines interleukin‐1β, tumour necrosis factor alpha and the receptor for interleukin‐2 with the basal mean concentrations (geometric mean; SD in parenthesis) of 172 (106; 148) ng/l, 46 (20; 67) ng/l and 9 (6; 7) pM, respectively. Interleukin‐6 was detectable in only 60 per cent of the specimens investigated. Interleukin‐2 and the receptor for interleukin‐6 were not detectable. We examined the statistical differences between the ratio R2/R1 (the changes which occurred during the resting period) and S2/S1 (the changes during the exposure to heat). We observed a significantly higher S2/S1 ratio for the tumour necrosis factor alpha in males, compared to the control ratio (2.5±2.4 versus 0.7±0.7,N=6,p<0.05 using the Wilcoxon signed rank test). Our results indicate that a situation which induced an elevated body temperature was associated with a rise in the concentration of the salivary tumour necrosis factor alpha.

The effects of psycho‐physical stress on immune function were evaluated by investigating the secretion rate of salivary interleukin‐1 beta (IL‐1β) during competitive rafting. Ten female and 20 male volunteers participated in this study. The concentration of IL‐1 β was determined in saliva using ELISA. We examined the statistical differences between T2/T1 (the changes observed during the training period) and C2/C1 (the changes before and after the competitive rafting) ratios by a Wilcoxon signed rank test. The ratio of IL‐1 β was significantly increased during competitive rafting (C2/C1 = 3.6; p < 0.01), compared to the control ratio (T2/T1 = 0.95; p < 0.01). The level of IL‐1 β at 8.00 a.m. decreased significantly between the first (11.9 ± 8.71 pmol/l) and the third day (7.9 ± 5.52 pmol/l) in all subjects (p < 0.01). In addition, the basal IL‐1 β levels significantly decreased between the beginning (8.00 a.m.) and the end of the day (6.00 p.m.), during the three consecutive days of collection (p < 0.01). These results may correspond to a circadian secretion rhythm of human saliva IL‐1 β. In conclusion, a disturbance of IL‐1 β salivary secretion is observed either after psycho‐physical stress or during intensive training. It may potentially affect the immune and endocrine status. Copyright © 2001 John Wiley & Sons, Ltd.

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The combination of ALT with AST is actually the best compromise in detecting cytolysis in untreated HCV-infected patients.

An improved turbidimetric assay for the measurement of C-reactive protein (CRP) has been evaluated in six laboratories on various analytical systems (Boehringer Mannheim Hitachi systems 704, 717, 747, 911, 917 and on Keysys® analyzer). Compared to the previous test version this assay has better transferability of results from instrument to instrument, less lipemia and gammopathy interference, a better linearity in the lower concentration range, the applicability of EDTA plasma or heparin plasma and a satisfactory recovery of target values according to CRM 470 standardization. The detection limit of the Tina-quant® CRP assay is 1.9 mg/1 CRP. Median values of CVs within run of lower than 2.5% and between day of lower than 3.3% were obtained. A survey with 50 human pool sera revealed a good transferability of results obtained in different laboratories and on different analytical systems. Method comparison studies between the Tina-quant® CRP assay and fixedtime nephelometry,. rate nephelometry and turbidimetry were in reasonable agreement (± 10%) throughout the entire measuring range. No drift effect was noticed with the tubing system on Boehringer Mannheim/Hitachi 747 analyzers. The reagent and calibration stability was extended to 12 weeks. The Tina-quant® CRP assay enables the precise, accurate, rapid and convenient determination of CRP used for routine clinical chemistry and STAT purposes.Zusammenfassung: Es wird über die Ergebnisse der multizentrischen Erprobung eines verbesserten turbidimetrischen Tests für die Messung von C-reaktivem Protein (CRP) in sechs Laboratorien auf Boehringer Mannheim/ Hitachi Analysensystemen und auf dem Keysys® Analysengerät berichtet. Im Vergleich zu der früher handelsüblichen Testversion weist der verbesserte Tina-quant® CRP Test eine bessere Transferabilität von Analysensystem zu Analysensystem und eine geringere Störanfälligkeit bei Lipämie und bei Gammopathien auf. EDTA-und Heparinplasmen können als Untersuchungsmaterial verwendet werden. Die Zielwerte im Referenzmaterial gemäß CRM 470 Kalibration werden gut gefunden. Die untere Nachweisgrenze mit Tina-quant® CRP liegt bei 1,9 mg/1 CRP; die medianen Variationskoeffizienten unter 2,5% innerhalb einer Serie sowie unter 3,3% von Serie zu Serie. Ein Ringversuch mit 50 Humanseren zeigte eine gute Übertragbarkeit der Ergebnisse zwischen verschiedenen Laboratorien und.verschiedenen Analysensystemen. Methodenvergleiche zwischen dem verbesserten Tina-quant® CRP Test und Fixed-Time Nephelometrie, kinetischer Nephelometrie und anderen turbidimetrischen Testverfahren zeigten eine maximale Abweichung von ± 10% über den gesamten Meßbereich. An Boehringer Mannheim/Hitachi 747 Geräten, die über ein Schlauchsystem verfügen, treten keine Drift-Effekte auf. Die Reagenzien-und Kalibrationsstabilität konnte auf 12 Wochen erhöht werden. Der Tina-quant ® CRP-Test erlaubt eine präzise, richtige und schnelle Bestimmung von CRP im klinisch-chemischen Routinelabor und in der Notfalldiagnostik.

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