RESUMO -O objetivo deste trabalho foi verificar se as cultivares de soja lançadas após 1990 para o Estado do Rio Grande do Sul continham, nos grãos, teores de proteína e de óleo diferentes dos contidos nos grãos das cultivares em uso anteriormente a esse ano. Na safra de 1996/97, foi realizado um estudo envolvendo genótipos produzidos em três locais do Rio Grande do Sul. O teor de óleo foi determinado pelo método de Soxlet, e o de proteína, pelo método de Kjeldahl. A maior parte das cultivares lançadas entre 1991 e 1996 apresentou menor teor de proteína e maior teor de óleo do que as mais antigas. A utilização das cultivares União e Industrial como genitores deve ter contribuído para o menor teor de proteína apresentado pelas cultivares. As linhagens experimentais apresentaram teor de proteína tão elevado quanto o das cultivares em cultivo desde antes de 1990, o que pode ter sido causado pela mudança de genitores. As correlações fenotípicas indicaram que existe uma associação negativa significativa somente entre os teores de óleo e de proteína.Termos para indexação: Glycine max, correlação genética, composição química. OIL AND PROTEIN CONTENT IN SOYBEAN GENOTYPES DEVELOPED AFTER 1990ABSTRACT -The objective of this work was to investigate whether oil and protein contents of soybean cultivars released after 1990 for the State of Rio Grande do Sul, Brazil, differ from the contents found in cultivars in use before that year. A study was carried out in 1996/97 involving cultivars recommended at different periods, produced at three sites of that state. Oil content was determined by the Soxlet method, and protein content was determined by the Kjeldahl method. It was found that most cultivars released between 1991 and 1996 showed lower protein content and higher oil content than older cultivars. The use of the União and Industrial cultivars as parents should be one of the main reasons to the lower protein content in these cultivars. The change of parents provided a recuperation in the protein content of new lines up to the level found in the cultivars released before 1990. Phenotypical correlations indicated that there is a significant negative association between oil and protein contents only.

The success of a genetic breeding program in a certain period can be assessed by the genetic gain observed. Genetic progress can be estimated from the multi environmental trials (MET) data which are routinely carried out by annual species breeding programs for the assessment of new commercial cultivars. A data set of 20 years of MET of advanced soybean lines derived from four breeding programs was used to estimate and to compare the genetic gains obtained for three soybean maturity groups (early, medium and late) in four cropping regions of the State of Rio Grande do Sul. The estimated yield gains ranged from 0.0 to 71.5 kg ha-1 year-1 (3.49% per year), depending on the maturity group and region, which suggests that the genetic breeding effort does not have a similar effect among the maturity groups or benefit the regions equally. There was no evidence of genetic progress for the early maturity group in any of the four regions, whereas gains in Regions I and IV were comparatively greater than those in Regions II and III. The objectives of the soybean breeding program in the region should be redirected. Since not all the experimental lines used to estimate genetic gains were commercially released, the reported genetics gains were achieved by the breeding programs rather than those achieved by the cropping systems.

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Employing a different culture strategy, we obtained a greatly improved frequency of embryo rescue in intersubgeneric soybean hybrids. Successful crosses were obtained in 31 different genotype combinations between nine Brazilian soybean lines as the female parents and 12 accessions from Glycine canescens, G. microphylla, G. tabacina and G. tomentella. The hybrid pod retention rate dropped to about 10% during the first 8 days after pollination and stayed largely unchanged up to the 20th day. Immature harvested seeds fell into three size groups: Group 1, smaller than 1.3 mm (mostly empty seed coats); Group 2, 1.9-5.0 mm; Group 3, larger than 5 mm (from selfing). A total of 90 putative hybrid embryos were rescued using a highly enriched B5 medium to nourish the newly dissected embryos. The growing embryos were then placed in a high osmotic, modified B5 medium to induce maturation and dormancy. Schenk and Hildebrandt medium was used to germinate the dormant, partially dehydrated, physiologically mature embryos. Approximately 37% of the rescued embryos developed into plantlets in vitro, and approximately 8% grew into mature plants in the greenhouse. Morphological, cytological and isoenzyme patterns confirmed the hybrid status of all seven mature plants, all of which were generated using G. tomentella G 9943 as the paternal parent. It was observed that all soybean lines crossed with G 9943 were capable of producing mature hybrid plants. There was no correlation between the initial size of Group 2 seeds and plant survival rate. The hybrids were cloned by grafting and treated with colchicine. One of the treated plants displayed chromosome doubling.