Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens. When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.

Biosecurity encompasses protecting against any risk through 'biological harm', not least being the economic impact from the spread of pest insects. Molecular diagnostic tools provide valuable support for the rapid and accurate identification of morphologically indistinct alien species. However, these tools currently lack standardization. They are not conducive to adaptation by multiple sectors or countries, or to coping with changing pest priorities. The data presented here identifies DNA barcodes as a very promising opportunity to address this. DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach. Species identifications were compared with the historical dataset obtained by PCR-RFLP of nuclear rDNA. There was 90 and 96% agreement between the methods for these species, respectively. Improvements included previous tussock moth 'unknowns' being placed to family, genera or species and further resolution within fruit fly species complexes. The analyses highlight several advantages of DNA barcodes, especially their adaptability and predictive value. This approach is a realistic platform on which to build a much more flexible system, with the potential to be adopted globally for the rapid and accurate identification of invasive alien species.

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We used laboratory experiments to show that the nonlethal presence of pumpkinseed sunfish (Lepomis gibbosus) results in smaller size at emergence, decreased growth and development rates, and lower fecundity of the dipteran Chironomus tentans. Smaller size at metamorphosis is often viewed as a cost of antipredator behavior. However, it may also partly result from a facultative change in life history in which prey increase their development rate to escape their risky larval habitat. To determine the mechanism responsible for this smaller size at emergence, we compared development rates of chironomid larvae raised in the absence and nonlethal presence of pumpkinseed sunfish. When we statistically controlled for effects of predators on larval growth rate, fish presence had no effect on development rate of female chironomids, but significantly slowed male development. These results clearly indicate that C. tentans does not increase its development rate as a means of escaping a risky habitat, and that smaller size at emergence is best viewed as a cost of larvae behaviorally avoiding fish predators. Observed sex differences in life history responses to predators may have important consequences for fitness.

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