We propose a new class of discrete generalized linear models based on the class of Poisson-Tweedie factorial dispersion models with variance of the form µ + φµ p , where µ is the mean, φ and p are the dispersion and Tweedie power parameters, respectively. The models are fitted by using an estimating function approach obtained by combining the quasi-score and Pearson estimating functions for estimation of the regression and dispersion parameters, respectively. This provides a flexible and efficient regression methodology for a comprehensive family of count models including Hermite, Neyman Type A, Pólya-Aeppli, negative binomial and Poisson-inverse Gaussian. The estimating function approach allows us to extend the Poisson-Tweedie distributions to deal with underdispersed count data by allowing negative values for the dispersion parameter φ. Furthermore, the Poisson-Tweedie family can automatically adapt to highly skewed count data with excessive zeros, without the need to introduce zero-inflated or hurdle components, by the simple estimation of the power parameter. Thus, the proposed models offer a unified framework to deal with under, equi, overdispersed, zero-inflated and heavy-tailed count data. The computational implementation of the proposed models is fast, relying only on a simple Newton scoring algorithm. Simulation studies showed that the estimating function approach provides unbiased and consistent estimators for both regression and dispersion parameters. We highlight the ability * Generalized linear models (GLMs) (Nelder and Wedderburn;1972) have been the main statistical tool for regression modelling of normal and non-normal data over the past four decades. The success enjoyed by the GLM framework comes from its ability to deal with a wide range of normal and non-normal data. GLMs are fitted by a simple and efficient Newton score algorithm relying only on second-moment assumptions for estimation and inference. Furthermore, the theoretical background for GLMs is well established in the class of dispersion models (Jørgensen; 1987, 1997) as a generalization of the exponential family of distributions. In particular, the Tweedie family of distributions plays an important role in the context of GLMs, since it encompasses many special cases including the normal, Poisson, non-central gamma, gamma and inverse Gaussian.In spite of the flexibility of the Tweedie family, the Poisson distribution is the only choice for the analysis of count data in the context of GLMs. For this reason, in practice there is probably an over-emphasis on the use of the Poisson distribution for count data. A well known limitation of the Poisson distribution is its mean and variance relationship, which implies that the variance equals the mean, referred to as equidispersion. In practice, however, count data can present other features, namely underdispersion (mean > variance) and overdispersion (mean < variance) that is often related to zero-inflation or a heavy tail. These departures can make the Poisson distribution unsuitable, or at lea...
Huanglongbing (HLB) is a disease associated with the bacteria “Candidatus Liberibacter spp.” and has been devastating citrus orchards around the world. Its management involves control of the insect vector, the Asian citrus psyllid Diaphorina citri Kuwayama. However, the indiscriminate use of chemicals has caused pest outbreaks and eliminated the natural enemies of the vector, such as the parasitoid Tamarixia radiata (Waterston), the main agent for biological control of D. citri. This study assessed the lethal and sublethal effects of insecticides recommended for integrated production of citrus on the parasitoid T. radiata. When adult parasitoids were exposed to residues of 25 insecticides, 20% of them, i.e., gamma-cyhalothrin, etofenprox, azadirachtin, tebufenozide and pyriproxyfen, were considered as harmless (Class 1), 12% as slightly harmful (Class 2), 12% as moderately harmful (Class 3) and 56% as harmful (Class 4), according to the classification proposed by the IOBC/WPRS. Afterward, 14 insecticides (5 harmless and 9 harmful) were sprayed on the parasitoid pupae. Of the 14 insecticides tested, only the organophosphates dimethoate and chlorpyrifos affected the parasitoid emergence. The effects of insecticides on the parasitism capacity of adults exposed to residues of azadirachtin, etofenprox, gamma-cyhalothrin, pyriproxyfen and tebufenozide (harmless) were also evaluated. Tebufenozide and gamma-cyhalothrin affected the parasitism of the F0 generation, but did not affect the emergence of the F1 and F2 generations. Therefore, for an effective IPM program, selective insecticides or harmful pesticides to adult parasitoids could be used in the field, provided that the adults do not occur naturally and the chemical applications do not coincide with parasitoid releases.
RESUMO -O uso do programa de Cornell indica a necessidade de estudos sobre a degradação dos diferentes componentes da matéria seca (MS). O objetivo deste trabalho foi avaliar a cinética ruminal da MS e das frações fibra em detergente neutro (FDN), proteína bruta (PB) e proteína insolúvel em detergente neutro (PIDN) de amostras de capim-Tanzânia (Panicum maximum Jacq.) obtidas por simulação de pastejo, coletadas em três épocas do ano (verão, outono e primavera), em pastagens irrigadas submetidas a três níveis de resíduo pós-pastejo (baixo, médio e alto). Para cada período de incubação, foram usados três animais fistulados no rúmen. Os tempos de incubação foram: 0, 3, 6, 9, 12, 24, 30, 48, 60, 72, 96 horas. No resíduo do material incubado, foram determinados os teores de FDN, PB e PIDN, utilizando-se NIRS. O nível de resíduo baixo foi o tratamento que apresentou maior redução de degradabilidade potencial entre o verão (81,41%) e a primavera (74,96%). A fração prontamente solúvel da proteína pode ser maior que 40% da proteína degradável no rúmen (PDR), que variou entre 76,36 e 62,05% da PB. A fração indegradável da FDN (C) é maior que a estimada pelo CNCPS a partir dos teores de lignina/FDN multiplicados por 2,4. Os resultados deste trabalho indicam que o fator de multiplicação deve estar entre 2,91 e 3,35. A fração C do PIDN (12,11 a 17,08%) é maior que a calculada em laboratório como nitrogênio aderido à fibra em detergente ácido (N-FDA) (6,48 a 10,7%). Quanto maior o teor de PB e de PIDN, pior a degradabilidade da PB. Palavras-chave: CNCPS, degradabilidade, FDN, irrigação, NIRS, PDR Ruminal Kinectics of Irrigated Tanzaniagrass under Three Post Grazed Stubbles IntensitiesABSTRACT -The objective of this work was to study DM, NDF, CP and IPND ruminal kinectics of irrigated Tanzaniagrass (Panicum maximum Jacq.). Simulated grazed samples were collected on three periods of the year (spring, summer and fall), under three post grazed stubbles intensities (low, medium and high). Three rumen cannulated animals were assigned to a 3 x 3 Latin square design to determine the in situ degradability until 96 hours of incubation. NDF, CP and IPND were determined by NIRS in the residue remaining in the bags. Forage quality was the highest in the summer. The low post grazed stubble treatment showed the highest increase in degradability of all fractions from spring (74.96%) to summer (81.41%). The soluble protein fraction showed values up to 40% of the RDP, which ranged from 62 to 76% CP. NDF C fraction was underestimated by CNCPS. Data of this research suggest that the %lignin/%NDF should be multiplied by a factor from 2.91 to 3.35. CNCPS overestimated the IPND C fraction (12.11 to 17.08%), as compared to the wet lab analysis value, as regarded to be the N-ADF fraction (6.48 to 10.7%). CP and IPND degradability decreased, as the CP and IPND contents increased. IntroduçãoNa busca da sincronização entre a degradação ruminal de carboidratos e proteína, faz-se necessário determinar as taxas de degradação da matéria seca e das diferen...
The Asian citrus psyllid (ACP) Diaphorina citri is the main vector of the bacteria associated with Huanglongbing, and can exploit more than 50 species of the family Rutaceae as hosts. The number of possible hosts is even higher if plant varieties are included. This study evaluated the influence of different combinations of scion and rootstock citrus varieties on the development and feeding of ACP. Survival rates for the egg stage were highest on the Valencia and Sicilian varieties, both grafted on Sunki mandarin, with means of 87.99 and 87.98%, respectively; and lowest (67.63%) on Hamlin 9 Rangpur lime. The lowest levels of both nymphal and total viability (egg-adult) were obtained on Hamlin, regardless of the rootstock used. The total development time (egg-adult) ranged from 17.92 to 19.33 days for the Pêra 9 Sunki and Hamlin 9 Swingle combinations, respectively. Cluster analysis separated the hosts into two groups, the first consisting of the combinations of the Hamlin variety, and the second group formed by the other varieties. The highest food value (assessed by the area of honeydew produced) was observed for the orange scion varieties, and among these, the highest value was observed on Valencia (0.902 cm 2); the smallest honeydew area was obtained on Ponkan (0.269 cm 2). The rootstocks did not affect the feeding behavior of D. citri. The results of this study could aid in the development of management techniques, mainly in providing information for the installation of new citrus groves and assistance in crop-improvement research.
Genomic evaluations have revolutionized dairy cattle breeding, and the demand for embryos produced from very young heifers with high genetic merit has increased over time. The combination of low oocyte recovery, young age of donors, and milk production status can make the in vitro embryo production (IVP) of Holstein cattle incredibly challenging. Several factors need to be coordinated to obtain a live calf from an IVP embryo, but the quality of the oocyte at the start of the process is one of the key factors. Aspects related to oocyte quality, laboratory quality control, embryo quality and recipient selection are addressed here, based on the measures that the RuAnn Genetics Laboratory (Riverdale, California, USA) adopted in the last 12 years, with the goal of improving production of live, healthy calves from Holstein embryos. Follicular wave synchronization and stimulation with follicular stimulating hormone (FSH) is necessary to improve oocyte quality and consequently embryo production. Laboratory quality control and the use of high-quality supplies are essential to reduce variability in production and facilitate identification of other factors that might interfere with embryo production. High pregnancy rates can be achieved with good quality embryos selected at optimal time and stage of development, transferred by an experienced embryo transfer (ET) technician, to well managed recipients 7 or 8 days after estrus. Attention to detail at every step of the process is crucial to success.
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