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A series of physical model experiments was performed to investigate the hydrodynamic responses of a semi-submersible offshore fish farm in waves. The structural configuration of the fish farm primarily refers to that of the world's first offshore fish farm, Ocean Farm 1, developed by SalMar in Norway. The mooring line tension and motion response of the fish farm were measured at three draughts. The study indicated that the tension on the windward mooring line is greater than that on the leeward mooring line. As the wave height increases, the mooring line tension and motion responses including the heave, surge, and pitch exhibit an upward trend. The windward mooring line tension decreased slightly with increasing draught. The existence of net resulted in approximately 42% reduction in mooring line tension and approximately 51% reduction in surge motion. However, the heave and pitch of the fish farm increased slightly with the existence of net. It was found that the wave parameters, draught, and net have noticeable effect on the hydrodynamic response. Thus, these factors are suggested to be considered in structural designs and optimization to guarantee the ability of the fish farm to resist destruction and ensure safety of workers during intense waves. weakness of development. The floaters and columns for a fish farm could be ideally used as a wave energy converter platform. The aquaculture platform of sufficient dimensions can support several wind turbines and realize self-power supply of the offshore fish farm. Overall, both the converter platform of wave energy and offshore wind turbine can be combined with this kind of new type of offshore aquaculture facility. Due to its superior performance, the offshore fish farm considered is accepted and widely used all over the world. Hence, fundamental research on the offshore fish farm is very critical, especially in the initial stage.

In order to assess the digestive physiological capacity of the American shad Alosa sapidissima and to establish feeding protocols that match larval nutritional requirements, we investigated the ontogenesis of digestive enzymes (trypsin, amylase, lipase, pepsin, alkaline phosphatase, and leucine aminopeptidase) in larvae, from hatching to 45 days after hatching (DAH). We found that all of the target enzymes were present at hatching, except pepsin, which indicated an initial ability to digest nutrients and precocious digestive system development. Trypsin rapidly increased to a maximum at 14 DAH. Amylase sharply increased until 10 DAH and exhibited a second increase at 33 DAH, which coincided with the introduction of microdiet at 30 DAH, thereby suggesting that the increase was associated with the microdiet carbohydrate content. Lipase increased until 14 DAH, decreased until 27 DAH, and then increased until 45 DAH. Pepsin was first detected at 27 DAH and then sharply increased until 45 DAH, which suggested the formation of a functional stomach. Both alkaline phosphatase and leucine aminopeptidase markedly increased until 18 DAH, which indicated intestinal maturation. According to our results, we conclude that American shad larvae possess the functional digestive system before mouth opening, and the significant increases in lipase, amylase, pepsin, and intestinal enzyme activities between 27 and 33 DAH suggest that larvae can be successfully weaned onto microdiets around this age.

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