Physiological response of juvenile turbot (Scophthalmus maximus. L) during hyperthermal stress

Jia, Yudong; Chen, Xiatian; Wang, Zhenyong; Meng, Zhen; Huang, Bin; Guan, Changtao

doi:10.1016/j.aquaculture.2020.735645

Cited by 30 publications

(14 citation statements)

References 69 publications

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“…In fact, fish exposed to short-term changes of temperature and ammonia, similar to those that can possibly occur in a commercial farm, were characterized by higher levels of this hormone respect to control already after 30 min of treatment. Increases of cortisol due to changes in these two environmental factors have been already reported in other species as black seabream ( Spondyliosoma cantharus ), blunt snout bream ( Megalobrama amblycephala ), brook trout ( Salvelinus fontinalis ), turbot ( Scophthalmus maximus ) and common carp ( Cyprinus carpio ) [ 59 , 67 , 68 , 69 , 70 ]. Acute change in salinity failed to increase cortisol significantly confirming the euryhalinity of the gilthead seabream [ 71 , 72 ].…”

Section: Discussionmentioning

confidence: 91%

Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Sparus aurata) Juveniles: A Multidisciplinary Study

Zarantoniello

Bortoletti

Olivotto

et al. 2021

Animals

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The present study aimed to investigate the acute response of gilthead seabream (Sparus aurata) juveniles exposed to temperature, salinity and ammonia stress. Radioimmunoassay was used to evaluate cortisol levels, whereas insulin-like growth factors (igf1 and igf2), myostatin (mstn), heat-shock protein 70 (hsp70) and glucocorticoid receptor (gr) gene expression was assessed trough Real-Time PCR. The presence and localization of IGF-I and HSP70 were investigated by immunohistochemistry. In all the stress conditions, a significant increase in cortisol levels was observed reaching higher values in the thermic and chemical stress groups. Regarding fish growth markers, igf1 gene expression was significantly higher only in fish subjected to heat shock stress while, at 60 min, igf2 gene expression was significantly lower in all the stressed groups. Temperature and ammonia changes resulted in a higher mstn gene expression. Molecular analyses on stress response evidenced a time dependent increase in hsp70 gene expression, that was significantly higher at 60 min in fish exposed to heat shock and chemical stress. Furthermore, the same experimental groups were characterized by a significantly higher gr gene expression respect to the control one. Immunostaining for IGF-I and HSP70 antibodies was observed in skin, gills, liver, and digestive system of gilthead seabream juveniles.

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Section: Discussionmentioning

confidence: 91%

Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Sparus aurata) Juveniles: A Multidisciplinary Study

Zarantoniello

Bortoletti

Olivotto

et al. 2021

Animals

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“…Similar results were also reported in other aquatic animals. DNA damage in pufferfish was shown to occur when the temperature rose to 34 °C [ 83 ], and increased apoptosis rates were confirmed in the hepatocytes of juvenile turbot after heat stress [ 83 , 84 ]. In addition, programmed cell death caused by heat stress was also reported in other aquatic animals, including American oyster and largemouth bass [ 84 , 85 , 86 ].…”

Section: Discussionmentioning

confidence: 99%

“…In brief, SOD can catalyze the dismutation of superoxide radicals and prevent an organism from suffering further damage. SOD level is used to reflect antioxidant status, and the activation of SOD has been reported in the heat shock response of teleosts [ 79 , 84 ]. T-AOC level represents the whole antioxidant capabilities of an organism, including enzymatic antioxidants (e.g., SOD, CAT) and non-enzymatic antioxidants (e.g., GSH, ascorbate, hypotaurine) [ 93 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

Yan

Qiao

et al. 2022

IJMS

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As an economically important flatfish in Asia, Japanese flounder is threatened by continuously rising temperatures due to global warming. To understand the molecular responses of this species to temperature stress, adult Japanese flounder individuals were treated with two kinds of heat stress—a gradual temperature rise (GTR) and an abrupt temperature rise (ATR)—in aquaria under experimental conditions. Changes in histopathology, programmed cell death levels and the oxidative stress status of gills were investigated. Histopathology showed that the damage caused by ATR stress was more serious. TUNEL signals confirmed this result, showing more programmed cell death in the ATR group. In addition, reactive oxygen species (ROS) levels and the 8-O-hDG contents of both the GTR and ATR groups increased significantly, and the total superoxide dismutase (T-SOD) activities and total antioxidant capacity (T-AOC) levels decreased in the two stressed groups, which showed damage to antioxidant systems. Meanwhile, RNA-seq was utilized to illustrate the molecular mechanisms underyling gill damage. Compared to the control group of 18 °C, 507 differentially expressed genes (DEGs) were screened in the GTR group; 341 were up-regulated and 166 were down-regulated, and pathway enrichment analysis indicated that they were involved in regulation and adaptation, including chaperone and folding catalyst pathways, the mitogen-activated protein kinase signaling (MAPK) pathway and DNA replication protein pathways. After ATR stress, 1070 DEGs were identified, 627 were up-regulated and 423 were down-regulated, and most DEGs were involved in chaperone and folding catalyst and DNA-related pathways, such as DNA replication proteins and nucleotide excision repair. The annotation of DEGs showed the great importance of heat shock proteins (HSPs) in protecting Japanese flounder from heat stress injury; 12 hsp genes were found after GTR, while 5 hsp genes were found after ATR. In summary, our study records gill dysfunction after heat stress, with different response patterns observed in the two experimental designs; chaperones were activated to defend heat stress after GTR, while replication was almost abandoned due to the severe damage consequent on ATR stress.

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“…Cultivated organisms have the ability to overcome stress resulting from changes in their external environment to some extent, but above a certain threshold level stress can result in reduced physiological activity (Park et al, 2011). In general, the levels of hepatic markers rise dramatically under stress and are considered indicative of a key response to stress in teleosts (Jia et al, 2020). When fish are exposed to stress, corticotrophin-releasing hormone is secreted from the hypothalamus of the brain to the pituitary gland, and adrenocorticotropic hormone is secreted from the pituitary gland, resulting in increased cortisol secretion from the adrenal glands (Cockrem et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In animals, thermal stress increases levels of HSPs and stress hormones (Iwama et al, 1999;Sharma et al, 2013). HSP family members play a critical role in unstressed and stressed cells as molecular chaperones responsible for the repair of damaged proteins and for the folding of new proteins (Jia et al, 2020). HSP expression increases following cellular stress resulting from exposure to high temperatures and oxidative damage, and has been detected in every organism evaluated (Feder and Hofmann, 1999;Kregel, 2002).…”

Section: Andmentioning

confidence: 99%

A comparison of the physiological responses to heat stress of juvenile and adult starry flounder (Platichthys stellatus)

Lee

Yoon

Park

et al. 2021

Israeli Journal of Aquaculture - Bamidgeh

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Water temperature is the most important factor in fish farming as changes in water temperature cause physiological stress in fish. There have been few studies on physiological responses to high temperatures, which vary with aging. This study investigated the responses of juvenile and adult Platichthys stellatus to heat stress. Plasma cortisol, glucose, lactate, and lysozyme levels, antioxidant enzyme activities, and expression of heat shock proteins 70 and 90 (HSP70 and HSP90) of P. stellatus were determined at water temperatures of 16, 20, 24, 28, and 30°C. As a result, it was confirmed that several plasma parameters of adult were significantly higher than that of juvenile under heat stress. Plasma cortisol and glucose levels of adult were increased than juvenile at 24 and 28°C. Plasma lactate level of adult were higher than that of juvenile at 28°C. Comparisons of survival and physiological changes showed that juveniles have better thermal tolerance, resulting in a higher cumulative survival rate. Moreover, the relationship between thermal tolerance and HSP gene expression revealed that expression of HSP70 and HSP90 was significantly upregulated at 28°C in both juvenile and adult fish, and HSP70 expression was significantly higher in juvenile fish than in adult fish. It is judged that the adult's HSP70 activity was lower than juvenile, so the demand for plasma parameters for heat response was relatively high, whereas juvenile's HSP70 activity increased at 24 and 28°C, indicating a relatively stable value of plasma parameters. These results indicate that the thermal tolerance of juvenile fish is greater than that of adult fish, based on the differences in plasma parameters and HSP expression. These findings improve our understanding of age-related changes in P. stellatus during thermal stress and may help guide the management of fish farms.

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Physiological response of juvenile turbot (Scophthalmus maximus. L) during hyperthermal stress

Cited by 30 publications

References 69 publications

Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Sparus aurata) Juveniles: A Multidisciplinary Study

Salinity, Temperature and Ammonia Acute Stress Response in Seabream (Sparus aurata) Juveniles: A Multidisciplinary Study

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

A comparison of the physiological responses to heat stress of juvenile and adult starry flounder (Platichthys stellatus)

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