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The present study was carried out to investigate the impact of silver nanoparticles (AgNPs) on the growth of three different crop species, wheat (Triticum aestivum, var. UP2338), cowpea (Vigna sinensis, var. Pusa Komal), and Brassica (Brassica juncea, var. Pusa Jai Kisan), along with their impact on the rhizospheric bacterial diversity. Three different concentrations (0, 50 and 75 ppm) of AgNPs were applied through foliar spray. After harvesting, shoot and root parameters were compared, and it was observed that wheat was relatively unaffected by all AgNP treatments. The optimum growth promotion and increased root nodulation were observed at 50 ppm treatment in cowpea, while improved shoot parameters were recorded at 75 ppm in Brassica. To observe the impact of AgNPs on soil bacterial community, sampling was carried out from the rhizosphere of these crops at 20 and 40 days after the spraying of AgNPS. The bacterial diversity of these samples was analyzed by both cultural and molecular techniques (denaturing gradient gel electrophoresis). It is clearly evident from the results that application of AgNPs changes the soil bacterial diversity and this is further influenced by the plant species grown in that soil. Also, the functional bacterial diversity differed with different concentrations of AgNPs.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0567-7) contains supplementary material, which is available to authorized users.

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Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency.

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Residue management has become a new challenge for Indian agriculture and agricultural growth, as well as environmental preservation. The rice-wheat cropping system (RWCS) is predominantly followed cropping system in the Indo-Gangetic plain (IGP), resulting in generating a large volume of agricultural residue. Annually, India produces 620 MT of crop residue, with rice and wheat accounting for 234 MT of the surplus and 30% of the total. Farmers are resorting to burning crop residue due to the short window between paddy harvest and seeding of rabi season crops, namely wheat, potato, and vegetables, for speedy field preparation. Burning of residues pollutes the environment, thus having adverse effects on human and animal health, as well as resulted in a loss of plant important elements. This problem is particularly prevalent in rice-wheat-dominant states such as Punjab, Haryana, Uttarakhand, and Uttar Pradesh. If we may use in situ management as residue retention after chopper and spreader, sowing wheat with Happy seeder/zero drill/special drill with full residue load, full residue, or full residue load incorporation with conventional tillage, burning is not the sole approach for residue management. In addition, off-farm residues generated are being utilized for animal feed and raw materials for industries. While there are regional variations in many mechanization drivers and needs, a wide range of mechanization components can be transported to new places to fit local conditions. This article focuses on innovations, methods, and tactics that are relevant to various mechanization systems in particular geographical areas. This article also stresses the need for a thorough analysis of the amount of residue generated, residue utilization using modern mechanical equipment, and their positive and negative effects on crop yield and yield attributes, weed diversity, soil physic-chemical, biological properties, beneficial, and harmful nematode populations in the IGP, which will aid researchers and policymakers in farming research priorities and policy for ensuring sustainability in RWCS.

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