Expression of four phosphate transporter genes from Finger millet (Eleusine coracana L.) in response to mycorrhizal colonization and Pi stress

Abstract

Phosphorus (P) is a vital nutrient for plant growth and development, and is absorbed in cells with the help of membrane-spanning inorganic phosphate transporter (Pht) protein. Symbiosis with arbuscular mycorrhiza (AM) also helps in transporting P from the soil to plant and Pht proteins play an important role in it. To understand this phenomenon in Finger Mille plant, we have cloned four Pht genes from Finger millet, which shares the homology with Pht1 protein family of cereals. Expression pattern analysis during the AM infection indicated that EcPT4 gene was AM specific, and its expression was higher in roots where AM colonization percentage was high. The expression level of EcPT1-4 gene under the phosphorous (Pi) stress in seedlings was found to be consistent with its role in acquisition of phosphorus. Homology study of the EcPt proteins with Pht proteins of cereals shows close relationship. The findings of the study indicate that Pht1 family genes from finger millet can serve to be an important resource for the better understanding of phosphorus use efficiency.

Rhizosphere Microbiome of Arid Land Medicinal Plants and Extra Cellular Enzymes Contribute to Their Abundance

“…Similarly, phosphatase degrades phosphoric acid monoesters into phosphate ions and alcohol [73]. Phosphate is one of the most important macronutrients of plant and plays diverse roles in plant growth and development, including root development and colonization of rhizospheric microbes [4,74,75]. Therefore, the presence of cellulase, glucosidase, and phosphatase in rhizosphere possess considerable significance.…”

“…However, in such harsh climatic conditions, endemic micro-symbionts are of great importance for plant survival [2]. Understanding the role of microbial communities and their association with plants during their growth, development, and extreme conditions in arid environments are of considerable interest to ecologists [3][4][5]. The microorganisms that are predominantly present in the rhizosphere have been shown to play a role in the transport of mineral nutrients, secretion of secondary metabolites, and mitigation of abiotic and biotic stresses [6][7][8][9][10].…”

Comprehensive evaluation of candidate reference genes for real-time quantitative PCR (RT-qPCR) data normalization in nutri-cereal finger millet [Eleusine Coracana (L.)]

“…A total of 85,243 genes from this genome sequencing data, predicted as stress related transcription factors/genes, calcium transporters accumulation genes and C 4 photosynthetic pathway genes, make it worthwhile to validate the function of the genes for understanding their role in various biological processes. So far most of the RT-qPCR analyses in finger millet have used reference genes either from heterologous plant species or from the native finger millet without any systematic normalization validation studies [ 23 – 34 ]. This becomes a serious hindrance in precise analysis of the RT-qPCR data and requires a comprehensive evaluation of the reference genes in finger millet under various experimental conditions.…”

“…So far most of the finger millet gene-expression studies have relied on conventional reference genes including EcActin [ 23 – 28 ], EcEF-1a [ 24 ], and EcTUB [ 29 – 34 ] for normalization studies under various experimental conditions. However, several reports have confirmed the instability in expression of conventional reference genes under various experimental conditions [ 35 , 36 ].…”

重金属矿区深色有隔内生真菌资源及其生态学功能

“…和担子菌门并列在真菌界下 [2] . 目前的研究认为AM是 一类古老的共生现象, 其起源可追溯到泥盆纪, 在植物 从水生到陆生的演化过程中起到了重要作用 [3] , AMF 在与植物的共进化过程中与植物逐渐形成了更加紧密 的关系, 例如AMF进化成为一类专性生物活体营养的 真菌(至今不能对其进行纯培养), 一些与植物营养相 关的基因, 如磷酸盐转运蛋白家族的某些基因必须要 有AMF在根上定殖才能表达 [4,5] . 一些在自然界中没有AMF定殖的所谓"非菌根"植物 也常被发现其根系上有DSE定殖 [6] .…”