Materials and methods
MaterialsStudy material included F 1 hybrid tomato (Riva), pepper (Ergenekon), and eggplant (Pera) cultivars sensitive to V.d.; AMF isolates of Glomus intraradices and G. mosseae, which are known to have high relative mycorrhizal dependency with these cultivars; and the pathogen V.d., which was isolated from eggplant with a high level of virulence.
This study evaluated seedling traits (shoot/root length, fresh and dry weights of shoots/roots, number of leaves, shoot diameter), phosphorus (P) content, mycorrhizal colonization (MC), and relative mycorrhizal dependency (RMD) of 21 common bean genotypes inoculated by 4 different arbuscular mycorrhizal fungi (AMF) [Glomus intraradices (Gi), Glomus mosseae (Gm), Gigaspora margarita (Gim), and commercial AMF (cAMF)] in a growth chamber under controlled growing conditions. With the exception of shoot diameter, inoculated plants had better seedling traits than noninoculated plants. P content of shoot was also higher in inoculated plants than in noninoculated plants. Of the 20 genotypes and Nadide cv. examined, 5 responded positively and 3 responded negatively to inoculation with all AMF types. Great variations in RMD and MC were observed, with the highest RMD and MC in the T120 (69.54%) and T71 (81.09%) genotypes and the lowest in the V77 (1.26%) and V78 (31.42%) genotypes. Positive correlations were found between RMD and seedling traits, with the exception of shoot length as well as root length, P content, and colonization. MC was significantly correlated only with P content.
Approximately 30% of plant species of Turkey, which is among the richest countries in terms of biodiversity, has been endemic. Wild rhubarb (Rheum ribes L.) is a wild vegetable grows especially in the eastern region of Turkey and is an endemic species.In this study, genetic relationships among 80 wild rhubarb genotypes collected from some regions of Lake Van Basin, which are in the distribution area, were tried to be determined by iPBS marker system. At the same time, a commercial variety of R. rhabarbarum, which is a cultivated species, was used for checkas control. PCR studies were conducted with 23 iPBS primers to determine genetic relationships, and a total of 340 scorable bands were obtained. 100% polymorphism rate was obtained from all primers studied. While the average PIC value was found to be 0.90, the highest value was found to be 0.97 from the primer # 2220. It was determined that the genotypes were divided into 3 basic groups in the dendogram created with UPGMA based on Jaccard similarity coefficient.
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