Transferability of Cucurbita SSR markers for genetic diversity assessment of Turkish bottle gourd (Lagenaria siceraria) genetic resources

Yıldız, Mehtap; Cuevas, Hugo E.; Şensoy, Suat; Erdinç, Çeknas; Baloch, Faheem Shehzad

doi:10.1016/j.bse.2015.01.006

Cited by 30 publications

(22 citation statements)

References 24 publications

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“…Mahlong, personal communication). This indicates that germplasm collection programs should be based on morphological variation rather than geographical background (Yildiz et al, 2015). Results of this study are in agreement with the findings of Bhawna et al (2015b), Sarao et al (2013), Xu et al (2011), andYetis xir et al (2008) who reported that clustering of bottle gourd genotypes was independent of geographical location.…”

Section: Discussionsupporting

confidence: 92%

“…These values are comparatively higher than those reported by Sarao et al (2013) with 0.23 to 0.73 and Xu et al (2011) with 0.11 to 0.72 (mean = 0.4). Yildiz et al (2015) reported PIC values of 0.12 to 0.52 (mean = 0.15) in bottle gourd, whereas Bhawna et al (2015b) found PIC values of 0.05 to 0.54 (average = 0.33) using 19 SSR markers. The PIC defines a relative measure of the informativeness of a marker or discriminatory power of a polymorphic marker, which depends on the number of alleles and relative frequency of an allele in the population (Bhawna et al, 2015b;Gaikward et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

“…Koffi et al (2009) reported the number of alleles per locus varying from 1.0 to 1.4 with a mean of 1.2 suggesting a low allelic richness. Yildiz et al (2015) and Bhawna et al (2015b) reported the number of alleles ranging from 1 to 5 (mean = 1.64) and 2 to 4 alleles (mean = 2.84) in bottle gourd, respectively. In the current study, the number of effective alleles ranged from 1.58 to 6.14 with a mean of 3.1.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Mashilo

Shimelis²,

Odindo³

et al. 2016

horts

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Bottle gourd [Lagenaria siceraria (Molina) Standl.] landraces are widely grown in South Africa, and genetic diversity analysis is necessary to identify promising genotypes for breeding or systematic conservation. Sixty-seven diverse bottle gourd landraces were genotyped using 14 selected simple sequence repeat (SSR) markers. The number of alleles detected per marker ranged from 4 to 11, with a total of 86 putative alleles being amplified. Allele sizes ranged from 145 to 330 base pair (bp). Number of effective alleles (Ne) ranged from 1.58 to 6.14 with a mean of 3.10. Allelic richness varied from 3.00 to 8.90 with a mean of 5.23. Expected heterozygosity (He) values ranged from 0.37 to 0.84 with a mean of 0.65. The mean polymorphic information content (PIC) was 0.57. Jaccard’s coefficient of similarity values ranged from 0.00 to 1.00, with a mean of 0.63. Analysis of molecular variance (AMOVA) revealed that 79%, 17%, and 4% of the variation in bottle gourd landraces was attributable to among landraces, within landraces, and between populations, respectively. The study established the existence of considerable genetic diversity among South African bottle gourd landraces. Unique landraces such as BG-4, BG-6, BG-8, BG-9, and BG-15 from cluster I; BG-55, BG-42, BG-57, and BG-58 from cluster II; BG-28, BG-23, BG-29, and BG-34 from cluster III were selected based on their highest dissimilarity index. These could be useful for bottle gourd breeding and systematic conservation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Mashilo

Shimelis²,

Odindo³

et al. 2016

horts

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“…SSR molecular markers have been proven very useful in diversity analysis due to high polymorphism levels and their codominant nature [25]. SSRs have been used for diversity analyses in many medicinally and commercially important crops like Tinospora cordifolia [42], Aloe vera [43], Lagenaria siceraria [26], Ziziphus jujube [44], Prunus armeniaca [45], Eragrostis tef [46], Sphenostylis stenocarpa [47] etc.…”

Section: Correlation Analysismentioning

confidence: 99%

“…Microsatellites have been applied to many commercially important plant species and have been proven to be very useful [26,27].…”

Section: Introductionmentioning

confidence: 99%

Validation and Transferability of Simple Sequence Repeats (Ssr’s) From Some Species of Acacia Genus to Acacia Nilotica L.

Yadav

Kumar

et al. 2016

Int J Pharm Pharm Sci

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Objective: In present study genetic transferability of SSR's from related Acacia species to Acacia nilotica was evaluated along with its genetic diversity analysis from north Indian region.Methods: A total of 30 primers selected from 5 different Acacia species were screened for amplification and polymorphism. Dendrogram and 2 D plot were constructed using NTSys PC version 2.02e. Different diversity parameters like Polymorphism information content (PIC), alleles per primer, number (no.) of amplicons were also calculated for each primer pair. Results: SSRs from Conclusion:Geographical and climatic conditions showed a great impact on genetic diversity. The results indicated high transferability of genomic resources from related species and will facilitate more studies to characterize the relatively less studied Acacia nilotica genome.

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Advances in Improvement of Pumpkin and Squashes

Dhatt

Sharma

Kaur

2020

Accelerated Plant Breeding, Volume 2

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Transferability of Cucurbita SSR markers for genetic diversity assessment of Turkish bottle gourd (Lagenaria siceraria) genetic resources

Cited by 30 publications

References 24 publications

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Genetic Diversity of South African Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Landraces Revealed by Simple Sequence Repeat Markers

Validation and Transferability of Simple Sequence Repeats (Ssr’s) From Some Species of Acacia Genus to Acacia Nilotica L.

Advances in Improvement of Pumpkin and Squashes

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