Stenocereus eruca (Cactaceae), a prostrate cactus endemic to the Sonoran Desert, is thought to be highly clonal. We examined its clonal diversity and distribution: (1) at the population level, in four distinct populations along its distribution range; and (2) at a micro scale level, within a single population. Our objective was to evaluate the importance of sexual versus clonal recruitment through the use of RAPD markers. Contrary to previous field observations, clonal diversity was relatively high across the distribution range. This finding suggests that sexual recruitment is an important regeneration mechanism. The proportions of distinguishable genotypes (G/N = 0.83) and genotypic diversity (D = 0.987) were greater than in other clonal cacti, suggesting that clonal propagation is not the major regeneration mechanism. Autocorrelation analyses revealed a spatial genetic structure that may be the result of restricted gene flow (via pollen or seeds) and clonal propagation. A molecular variance analysis (AMOVA) indicated that most of the variation (66.3%) was found within and not across populations. Future studies on pollen and seed dispersal are needed to understand the role of the clonal habit in the mating system of S. eruca.

Abstract:The tropical montane cloud forests are recognized as one of the most biodiverse ecosystems. In spite of this, they are among the most threatened ecosystems in the world. This study integrates three ecological approaches generally studied separately: climate change scenery, ecological niche and population dynamics of Oreomunnea mexicana (an endangered and relict species), to understand how environmental change affects the population structure in the cloud forest that will allow its conservation. Potential distribution under future climatic scenarios of the species at national and regional levels was generated from the Maxent algorithm. Also, the current abundance, distribution and the ecological niche of the species were analyzed at the regional level. Changes in potential distribution under two climatic models suggest a habitat reduction from 36% to 55% nationally, and 2% to 9% at a regional level, for 2050 and 2070, respectively. The current distribution of the species is fragmented and consists of subpopulations that have spatial structures of aggregated populations and a size structure in reversed "J" form. The ecological niche of the species is highly specialized and sensitive to environmental changes. O. mexicana is a flagship species of biological and cultural importance to the region's inhabitants and could be fundamental to the conservation of tropical montane cloud forests.

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Quercus eduardii and Q. potosina are dominant oak species in Sierra Frı´a, Aguascalientes, Mexico. These species have been exploited for multiple purposes since the 16th century. Both species produce clonal offspring through root suckering and acorns through sexual reproduction. To understand clonality for the implementation of the most adequate actions for the conservation of these species, we addressed the following questions: (a) what is the spatial clonal structure of both species? (b) How much clonal and genetic diversity is maintained in these species? Random Amplified Polymorphic DNAs (RAPDs) were used as molecular markers for these analyses. Genets of both species have few ramets and these grow close the parent tree. Autocorrelation analyses at the ramet level showed an aggregated distribution at short distances and a random spatial distribution at larger distances. Also, at the genet level the autocorrelation analyses showed a random distribution. Clonal diversity was high in both species (Q. eduardii: D ¼ 0.963, G/N ¼ 0.60; Q. potosina: D ¼ 0.985, G/N ¼ 0.65). Genetic diversity was high within populations (Q. eduardii: H e ¼ 0.33 ± 0.11; Q. potosina: H e ¼ 0.35 ± 0.11). Low levels of genetic differentiation among populations were observed (Q. eduardii: / st ¼ 0.19, P < 0.002; Q. potosina: / st ¼ 0.13, P < 0.002). Both species maintain high levels of clonal and genetic diversity, probably due to successful sexual reproduction, which allows gene flow among populations. Conservation and/or reforestation programs must include seed collections and germplasm banks. Due to the small genet size and the high clonal diversity of these species, seeds can be collected in any place in Sierra Frı´a, Aguascalientes.

Stenocereus eruca is a prostrated, self-incompatible cactus endemic to the Sonoran Desert that regenerates primarily through clonal propagation. Clonal growth is expected to affect mate availability by influencing the number and spatial distribution of mating types. In this paper we examine the role of clonal growth on female fecundity through a series of pollination experiments in a population of S. eruca. We set up a pollen supplementation experiment using five distance treatments with pollen collected at 1, 10, 100, 1000 and 25000 m from receptor flowers during the years 2001 and 2002 and evaluated genetic sifmilarities between pairs of receptor-donor ramets through RAPD markers. Our data on fruit set, number of seeds/fruit, germination and overall fecundity revealed that S. eruca show a significant reduction in female fecundity when pollination occurs between ramets located at short distances (1 and 10 m), while genetic data showed high levels of similarity at those distances. The reduction in female fecundity is apparently a consequence of geitonogamy and inbreeding depression. Our data suggest that clonal growth and geitonogamy are likely to be partially responsible for the low levels of sexual reproduction and seedling recruitment observed in populations of S. eruca.

Cliff-dwelling plant species are highly specialized and adapted to a vulnerable, fragmented, and are mostly endemic, narrowly-distributed and threatened. As a contribution to the conservation efforts of endemic cliff-dwelling species, this study provides an overview of the effects of habitat loss on the abundance and distribution of Pachyphytum caesium (Crassulaceae) due to human disturbances. To achieve this objective, we first conducted a retrospective analysis from 2003–2013 to assess the effects of land use change on the abundance of P. caesium. Secondly, we estimate the abundance and distribution of P. caesium throughout the study area, as well as analyze the effect of rock-climbing activities on the density and population structure of P. caesium. The results suggest differences in population abundance among sites is due to the adverse effects of habitat loss. P. caesium presents a very restricted distribution with small and fragmented populations. In addition, guava agriculture has a significant impact on the chemical soil properties of the hillsides, causing a significant effect on the occurrence of P. caesium, while sport activities remove both the soil and the plants from the cliffs. According to the results, P. caesium is classified as a plant species with extremely small populations (PSESP), and it is highly vulnerable to habitat disturbance. Its conservation is thus a priority to ensure its permanence.

ResumenSe analizan los factores ambientales que determinan la abundancia y distribución de las diferentes especies comerciales y aprovechadas del género Pinus en el municipio de Capulálpam de Méndez, Oaxaca. Se utilizó la información de 636 sitios en áreas de aprovechamiento del inventario forestal del municipio llevado a cabo en 2013 para el Plan de Manejo 2015-2022, y 354 puntos adicionales de áreas con uso diferente al forestal. Se obtuvo información sobre la distribución espacial y análisis del nicho ecológico de los pinos, para lo cual se emplearon nueve variables climáticas y cuatro geográficas. Se encontraron 10 especies, de las cuales P. patula presentó mayor abundancia y P. chiapensis fue la menor. El análisis de correspondencia canónica indicó que la mayoría de los pinos analizados se asocian en mayor medida con las variables climáticas que con las geográficas a excepción de la latitud. El análisis de nicho mostró que las especies del género Pinus muestran alta marginalidad y baja tolerancia a procesos de cambio ambiental, donde una modificación al hábitat podría restringir o disminuir su abundancia y distribución, siendo P. chiapensis la especie de menor tolerancia y P. patula la de mayor amplitud de nicho.Palabras clave: amplitud, hábitat, índice de marginalidad, nicho ecológico, tolerancia. AbstRActIn this study, environmental factors that determine the abundance and distribution of commercial pine species in the municipality of Capulálpam de Mendez, Oaxaca, were analyzed. We used information pertaining to tree species abundance in 636 sites established in harvesting areas from the municipal forest inventory conducted in 2013 for the Management Plan 2015-2022, as well as 354 additional sites located in areas with different land use. Data of the spatial distribution and ecological niche analysis of the species were obtained, for which nine climatic and four geographic variables were used. Ten species of pine were recorded, of which Pinus patula showed the highest abundance and P. chiapensis the lowest. Canonical Correspondence Analysis indicated that most of the pine species are more highly associated with the climatic variables than with geographic variables, except for that of latitude. Niche analysis showed that species of the genus Pinus are highly marginal and of low tolerance to processes of environmental change. Minimal alterations to their habitat could restrict or reduce their abundance and distribution, with P. chiapensis being the least tolerant species and P. patula the species that presented the broadest niche.

Higher Education Institutions (HEI) play a fundamental role in the transition towards Environmental Education for Sustainability (EES). As a consequence, one of the most critical challenges is the need to know their level of incorporation into the environmental agenda. Therefore, an instrument was made and validated to determine the level of incorporation of Environmental Education for Sustainability into the environmental agenda of HEIs. For its construction, the dimensions of Institutional Identity, Teaching, Research, Extension/dissemination, and Linkage were considered, relying on a total of 17 items. Its validation was carried out through an expert review and expert judgment, and a pilot test was carried out to adapt it to the target population. The main result was an instrument that integrates the substantive and procedural functions of HEIs. Following the expert review, the instrument was improved according to their suggestions. The expert judgment showed an adequate content validity (Aiken’s V > 0.80; LL > 0.60). The pilot test also suggested that the understanding of instructions and items was adequate with an optimal value of internal consistency (Cronbach’s alpha of 0.862). An instrument that determines the level of incorporation of the EES in the substantive and procedural functions of HEIs is presented, valid in content, and with adequate levels of clarity and understanding of the target population.