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Urinary tract infections (UTIs) are severe public health problem and caused by mono- or poly-bacteria. Culture-based methods are routinely used for the diagnosis of UTIs in clinical practice, but those...

The combination of bio- and chemo-catalysts for sequential cascades has received considerable attention in analytical fields because of the regulable catalytic efficiency and selectivity under various physiological conditions. In this...

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A lack of rapid and dependable microbial identification platforms, as well as insufficient or overdue microbiological surveillance and corresponding resolutions implemented in clinical diagnosis and treatment, agricultural production, and the food industry, can seriously harm human health and reduce productivity in the food industry. Here, a two-dimensional Ni–Co bimetallic metal–organic framework nanozyme (2D-NCM) is prepared for the rapid and efficient discrimination of microbes including clinical pathogens and brewing fungi. The nanozyme 2D-NCM, which is synthesized via a facile layered double hydroxide in situ transformation strategy, exhibits enhanced peroxidase-like activity. The biocatalytic activity of 2D-NCM could be altered to different degrees via different interactions between 2D-NCM and microbes. By selection of diverse compositions of absorbance at different time points, the sensing unit, 2D-NCM, could provide multichannel information for microbial identification. The nanozyme-based platform prevents the tedious synthesis of multiple biosensing receptors and the demand for complicated operation/precise devices, resulting in lower cost, quicker response (within 0.5 h), higher throughput, and simpler handling without washing procedures. This study provides an alternative strategy to construct practicable, facile, and flexible MOF nanozyme based biosensing arrays for the identification of microbes, making an active contribution toward precision medicine, food safety, and environmental protection.

The widespread use of antibiotics has made multidrug-resistant bacteria (MDRB) one of the greatest threats toward global health. Current conventional microbial detection methods are usually time-consuming, labor-intensive, expensive, and unable to detect low concentrations of bacteria, which cause great difficulties in clinical diagnosis and treatment. Herein, we constructed a versatile biosensing platform on the basis of boric acid-functionalized porous framework composites (MOF@COF-BA), which were able to realize highly efficient and sensitive labelfree MDRB detection via fluorescence. In this design, MDRB were captured using aptamer-coated nanoparticles and the fluorescent probe MOF@COF-BA was tightly anchored onto the surface of MDRB due to interactions between boric acid groups and glycolipids on bacteria cells. Benefitting from the remarkable fluorescence performance of MOF@COF-BA, rapid and specific detection of MDRB, such as methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii (AB), was realized with a detection range of 20−10 8 CFU/mL (for both) and limits of detection of 7 CFU/mL (MRSA) and 5 CFU/mL (AB). The feasibility of using the developed platform to selectively detect MRSA and AB from complex urine, human serum, and cerebrospinal fluid samples was also demonstrated. This work provides a promising strategy for accurate MDRB diagnosis, avoiding serious infection using rational antibiotic therapy.