Ultrasensitive Detection of Multidrug-Resistant Bacteria Based on Boric Acid-Functionalized Fluorescent MOF@COF

Abstract

The widespread use of antibiotics has made multidrug-resistant bacteria (MDRB) one of the greatest threats toward global health. Current conventional microbial detection methods are usually time-consuming, labor-intensive, expensive, and unable to detect low concentrations of bacteria, which cause great difficulties in clinical diagnosis and treatment. Herein, we constructed a versatile biosensing platform on the basis of boric acid-functionalized porous framework composites (MOF@COF-BA), which were able to realize highly efficient and sensitive labelfree MDRB detection via fluorescence. In this design, MDRB were captured using aptamer-coated nanoparticles and the fluorescent probe MOF@COF-BA was tightly anchored onto the surface of MDRB due to interactions between boric acid groups and glycolipids on bacteria cells. Benefitting from the remarkable fluorescence performance of MOF@COF-BA, rapid and specific detection of MDRB, such as methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii (AB), was realized with a detection range of 20−10 8 CFU/mL (for both) and limits of detection of 7 CFU/mL (MRSA) and 5 CFU/mL (AB). The feasibility of using the developed platform to selectively detect MRSA and AB from complex urine, human serum, and cerebrospinal fluid samples was also demonstrated. This work provides a promising strategy for accurate MDRB diagnosis, avoiding serious infection using rational antibiotic therapy.

Rapid detection of Saccharomyces cerevisiae with boronic acid-decorated multivariate metal–organic frameworks and aptamers

“…Apt-MPs were prepared as described in our previous work. 28 Briefly, the aqueous suspension of streptavidin-modified magnetic particles (SA-MPs, 10 mg mL −1 , 100 μL) was mixed with a biotin-functionalized aptamer (100 μM, 9 μL) and incubated at 37 °C for 1 h. The excess aptamer was removed through magnetic separation and the prepared Apt-MPs redispersed in tris-(hydroxymethyl)-aminomethane/hydrochloric acid buffer (Tris-HCl, pH 7.4) after being washed several times with Tris-HCl.…”

Emerging Biomimetic Sensor Technologies for the Detection of Pathogenic Bacteria: A Commercial Viability Study

“…It was also shown that it is possible to couple MOFs and MIPs technologies for the efficient detection of bacteria . On the other hand, MOFs can also be coupled with aptamers to enhance their sensitivity to detect bacteria like S. aureus or Acinetobacter baumannii reaching a very low limit of detection in the range of 5–7 CFU/mL. , By encapsulating target molecules within their framework, MOFs have highly precise cavities, finely tuned for recognizing key bacterial elements. Moreover, their robust polymeric composition confers good stability to harsh environmental conditions, rendering them adept for Point-of-Care (PoC) applications.…”