β-cyclodextrin modified graphene oxide-magnetic (MGC) nanocomposite as an innovative drug carrier was the first developed via an effective layer-by-layer-assembly method. Doxorubicin hydrochloride (DOX) and epirubicin hydrochloride (EPI) as model drugs were loaded onto the MGC via π-π stacking, hydrogen bond and hydrophobic interaction. The MGC exhibits remarkably higher loading capacity for DOX (909.09 mg/g) and EPI (781.25 mg/g) than magnetic graphene oxide (MG). The release profiles of drug are pH-sensitive which would control the release in acidic cytoplasm of cancer cells. Furthermore, cellular uptake using fluorescein isothiocyanate (FITC) labeled MGC proves successful internalization of MGC into the cytoplasm of MCF-7 cells. The fluorescence images demonstrate that MGC/DOX, to a certain extent, displays a more excellent delivery and superior release than MGC/EPI, due to the chiral select function of β-cyclodextrin (β-CD). The pure MGC shows no obvious cytotoxicity while drug loaded MGC reveals significantly high potency of killing MCF-7 breast cancer cells, suggesting that multi-functionalized MGC is an efficient nanoplatform for targeted delivery and controlled release of stereoisomeric anticancer drugs for biomedical applications.
The discovery of a general strategy for organizing functional proteins into stable nanostructures with the desired dimension, shape, and function is an important focus in developing protein-based self-assembled materials, but the scalable synthesis of such materials and transfer to other substrates remain great challenges. We herein tackle this issue by creating a two-dimensional metal−protein hybrid nanofilm that is flexible and costeffective with reliable self-recovery, stability, and multifunctionality. As it differs from traditional metal ions, we discover the capability of Sn 2+ to initiate fast amyloid-like protein assembly (occurring in seconds) by effectively reducing the disulfide bonds of native globular proteins. The Sn 2+ -initiated lysozyme aggregation at the air/water interface leads to droplet flattening, a result never before reported in a protein system, which finally affords a multifunctional 2D Sn-doped hybrid lysozyme nanofilm with an ultralarge area (e.g., 0.2 m 2 ) within a few minutes. The hybrid film is distinctive in its ease of coating on versatile material surfaces with endurable chemical and mechanical stability, optical transparency, and diverse end uses in antimicrobial and photo-/electrocatalytic scaffolds. Our approach provides not only insights into the effect of tin ions on macroscopic selfassembly of proteins but also a controllable and scalable synthesis of a potential biomimic framework for biomedical and biocatalytic applications.
Metal
nanohybrids are fast emerging functional nanomaterials with
advanced structures, intriguing physicochemical properties, and a
broad range of important applications in current nanoscience research.
Significant efforts have been devoted toward design and develop versatile
metal nanohybrid systems. Among numerous biological components, diverse
proteins offer avenues for making advanced multifunctional systems
with unusual properties, desired functions, and potential applications.
This review discusses the rational design, properties, and applications
of metal–protein nanohybrid materials fabricated from proteins
and inorganic components. The construction of functional biomimetic
nanohybrid materials is first briefly introduced. The properties and
functions of these hybrid materials are then discussed. After that,
an overview of promising application of biomimetic metal–protein
nanohybrid materials is provided. Finally, the key challenges and
outlooks related to this fascinating research area are also outlined.
1D rare earth‐based nanomaterials have attracted significant attention due to their excellent photo/electro‐catalytic performance. The corresponding challenge is how to synthesize shape and size‐controlled nanostructures in an easy scale‐up way. Herein, the authors present a facile one‐step strategy to design 1D multifunctional protein‐encapsulated cerium oxide nanorods (PCNRs) by utilizing bovine serum albumin as an efficient biotemplate. Remarkably, the PCNRs exhibit high chemical and interfacial adhesion stability with intriguing properties, resulting in an exceptionally high activity towards H2 evolution and CO2 reduction. The photocatalytic activity of PCNRs to produce H2 is about 10 times higher than conventional CeO2 nanorods. The incorporation of rhodamine B into the PCNRs brings unprecedentedly high photocatalytic H2 evolution rate being 123 times higher than that of conventional CeO2 nanorods. Further the presence of the –NH2 groups on the PCNRs facilitated the adsorption and activation of CO2 and efficiently suppressed the proton reduction, and as a result, the PCNRs photocatalyst is highly active in converting CO2 to CO and CH4, with the evolution rates being 50 and 83 times higher than those of conventional CeO2 nanorods, respectively. Achieving such efficient photocatalyst is a critical step toward practical production of high‐value renewable fuels using solar energy.
Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe 3 O 4 magnetic nanoparticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The characterization of drug delivery system was confirmed by Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM), X-Ray Diffraction (XRD), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). The adsorbed APTES on the magnetite surface (MNPs-APTES) was examined by FTIR. The TEM image showed that the average particle size is obtained to be about 26.7 nm for MNPs-APTES. The MR loading and release behavior of MNPs-APTES were studied and the results showed that up to 60% of the adsorbed drug was released within 4 h. In summary, the MNPs-APTES nanocarriers are based on the results, promising for targeted morin drug delivery.
Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe 3 O 4 magnetic nanopar-ticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The characterization of drug delivery system was confirmed by Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM), X-Ray Diffraction (XRD), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). The adsorbed APTES on the magnetite surface (MNPs-APTES) was examined by FTIR. The TEM image showed that the average particle size is obtained to be about 26.7 nm for MNPs-APTES. The MR loading and release behavior of MNPs-APTES were studied and the results showed that up to 60% of the adsorbed drug was released within 4 h. In summary, the MNPs-APTES nanocarriers are based on the results, promising for targeted morin drug delivery.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.