It is concluded that female blood donors with only two previous pregnancies can form clinically important granulocyte-reactive alloantibodies leading to fatal TRALI reactions in recipients. The sometimes devastating consequences of TRALI should prompt the development of strategies to prevent or reduce its incidence. Further research is warranted to investigate recipient and donor factors responsible for TRALI, including whether 5b (HNA-3a) alloantibodies are especially prone to cause severe reactions, and to better characterize the HNA-3a (5b) antigen, particularly at the molecular level.

No abstract

The aim of this study was to provide the morphological and morphometric data of the supraorbital foramina or notches related to sex, side, and the climatic conditions where the population lived. It was hypothesized that the distribution of the occurrence and location of these openings depends on climatic conditions in which the population lived. Orbits from 866 dried skulls obtained from three climatic regions: warm, temperate, and cold were examined. The examination concentrated on the configuration (notch/foramen) and on the distances to the reference points: nasion, frontomalare orbitale, infraorbital foramen and the superior orbital rim. In 14.3% of cases a smooth supraorbital rim was observed while different variants of the structures were observed in 85.7% of the cases. In cold climatic conditions, supraorbital foramina were found in the highest frequency (35.4%). In warm and temperate climates, the observed frequencies of supraorbital foramen were the lowest (18.8% and 19.9%, respectively). Frequency of supraorbital notches was the lowest of those skulls from a cold climate (44.0%) and the highest in those from a warm climate (59.0%). These results support the hypothesis that the occurrence of the supraorbital notches is greater in populations from warm compared with cold regions. This would provide a greater exit route for the neurovascular bundle and this may be related to the thermoregulatory processes in the supraorbital region. Furthermore, knowledge of precise locations of supraorbital structures is important when a supraorbital nerve block is given, for example, in the treatment of migraine headaches. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.

The anthropological analysis and assessment of the living conditions of historical populations should be comprehensive. Due to the scarcity of the well-preserved skeletal remains of a population buried at the cemetery in Wyszyński Street, Wrocław, each piece of information is particularly important in providing the complementary information about living and health conditions of late medieval and early modern inhabitants of Wrocław. This study aims to assess the living conditions of the late medieval and early modern Wrocław inhabitants. This aim was reached by analyzing the frequency of non-metrical cranial and postcranial traits, physiological stress indicators, and pathological lesions. The importance of these traits for population studies is also discussed. For 98 skeletons (22.4% male and 37.8% female) from a cemetery located in Wyszyński Street, (15th –18th centuries AD), the frequencies (p) of the following traits were analyzed: 38 non-metric cranial and 9 post-cranial traits, the morphological indicators of physiological stress (i.e. cribra orbitalia and enamel hypoplasia), caries and pathological lesions on bones. Among the non-metric cranial traits, only nine (23.7%) were not observed. The most frequent (from p= 0.30 to p=0.65) were foramen parietale, incisura frontalis accesoria seu foramen frontale accesorius, foramen supraorbitale, M3 mandibulare, ossicula suturae lambdoideae, and foramen mastoideum extrasuturale. Only three non-metric post-cranial traits were observed, but with low frequency (p=0.01 and 0.02). Cribra orbitalia was present in 37.0% of the skeletons while enamel hypoplasia was present in only 2.6%. Susceptibility to caries occurred in 68% of the cases while carries lesions occurred in 31.2%. Among pathological changes, the most frequent were degenerative changes (21.6%) and injuries (19.6%). Owing to the poorly preserved remains, the complete assessment of the living conditions of the population was difficult. The number of the observed traits would have probably increased, had the number of complete and well-preserved skeletons been higher. The results of the study suggest that the living conditions of the studied population were poor. It is hypothesized that the cemetery was a burial place for prisoners and victims of various epidemics that affected the city. But since the historical sources of this cemetery are scarce, it is hard to unequivocally state its purpose with certainty.

No abstract

These data may support the hypothesized association between size of the orbital opening and adaptation to different climatic conditions, but only in males.

No abstract

Malignant transformation is associated with enhancement of cell plasticity, which allows cancer cells to survive under different conditions by adapting to their microenvironment during growth and metastatic spread. Much effort has been devoted to understanding the molecular mechanisms of these processes. Although the importance of the extracellular matrix and of surface properties in these mechanisms is evident, the direct impact of distinct physical and chemical surfaces characteristics on cell fate remains unclear. Here, we have addressed this question using HT1080 fibrosarcoma cells as a model. To examine the relationship between surface topography, chemistry, and cell behavior, hydrophobic poly(butyl methacrylate-co-ethylene dimethacrylate) (BMA-EDMA) and hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (HEMA-EDMA) surfaces with three different topographies (microporous, nanoporous, and nonporous) were generated. These surfaces were then modified by photoinitiated grafting of three different methacrylate monomers to create surface chemistry gradients of either negatively (AMPS) or positively (META) charged or zwitterionic (MDSA) functionalities. Our results show that AMPS promotes cell spreading, but that META abolishes cell growth. META and MDSA grafted on microporous BMA-EDMA produced superhydrophilic surfaces with high globularity and elasticity, which modified the cell phenotype by inhibiting cell spreading, followed by loss of mesenchymal characteristics and a reduction in protein levels of the mesenchymal markers N-cadherin, beta-catenin, p120 catenin, and also of the adaptor proteins vinculin and paxillin that are associated with adhesion and cancer cell invasion. The effect was strengthened along the gradient, suggesting that the density of the functional groups plays a role in this process. On the nanoporous surface, only MDSA grafting resulted in a significant increase in cell number, a reduction in N-cadherin expression, increased beta-catenin and p120 catenin levels, as well as the appearance of the epithelial marker E-cadherin. This indicates that the cancer cells have a high plasticity that is triggered by the collaborative effect of physical and chemical surface properties.