In this study, we aimed to estimate the anatomical variations of the supraorbital notch or supraorbital foramen (SON/F) using three-dimensional computer tomography (3D-CT) volume rendering and evaluate these variations related to gender and side. One hundred and five adults (53 men and 52 women), aged 21 to 83 years, without any trauma or bone malformation of facial bones, were included in our study. Presence of SON/F, its shape, diameter in millimeters, and distance from the reference point were recorded. The most common presentation of the supraorbital passage was single SON and it was noted in 51.43% on the right and in 57.14% on the left skull side. The single SOF was noted in 3.81% on the right and 5.71% on the left skull side. Double notch was found in 1.90% on the right and in 0.95% on the left side, while double foramen was not found in subjects included in our study. SON/F was not found in 42.90% and 36.20% on the right and left side, respectively. The average SON diameter was 3.7 ± 0.9 mm, and the average SOF diameter was 1.7 ± 0.5 mm. The average distance of SON/F to the facial midline was 21.9 ± 3.1 mm. The average distance of SON/F to the temporal crest of the frontal bone was 26.0 ± 3.0 mm and to the frontozygomatic suture was 27.5 ± 2.6 mm. Statistically significant difference was observed between the right and left side for the distance from SON/F to frontozygomatic suture (p < 0.01). No significant difference was found between the left and right sides in the other measurements (p > 0.05). Statistically significant differences were found between males and females for some of these parameters. The results of this study provide useful information about the location of the supraorbital neurovascular bundle and may be used in preoperative evaluation.