The research had conducted the antibacterial activity of ethanol extract of Chromolaena odorata (L.) leaves by TLC Bioautography. It aimed to evaluate the antibacterial activity of the ethanol extract.The ethanol extract of leaves Chromolaena odorata (L.) was done by screening at the concentration of 0.1% on Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, then test bioautigrafi TLC and identification of chemical components. The results of screening showed that the ethanol extract of 0,1% concentration had an antibacterial activity. Meanwhile, TLC-Bioatography method resulted in 5 active spots with different Rf values 0
The main factors contribute to breast cancer development is the combination of exogenous and endogenous factors. Endogenous factors include both SIRT1 and NF-kB. Exogenous factor used in this research is 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3-3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) is a derivative of isoflavone generate from clove leaf oil. To examine the effect of EPI on SIRT1 and NF-kB expression in DMBA-induced Sprague Dawley (SD) rats, and the correlation between SIRT1 and NF-kB expressions. Tissue generated form 35 Sprague Dawley female rats aged 2 weeks old were used in this study. Those rats were divided into 7 groups (5 rats/group), namely normal control group; corn oil group; DMBA group; EPI treated groups with 1 mg/kgBW (EPI I), 2 mg/kgBW (EPI II), and 4 mg/kgBW (EPI III), respectively; and doxorubicin group. EPI and doxorubicin were administered from 1 st until 15 th week, while DMBA were administered from 3 rd until 9 th week. The paraffin block was prepared from all breast organ of the rats that terminated at the end of week 15 th. Examination of SIRT1 and NF-kB expression was performed using light microscope at 400x magnifications, after immunohistochemistry (IHC) staining. Expression level of SIRT1 and NF-kB were analyzed using IHC-profiler plug-in in ImageJ software. SIRT1 and NF-kB expression in EPI treated groups were not significantly different with the one in Doxorubicin group, but lower than DMBA group (p=0.000). There was a positive correlation between SIRT1 and NF-kB expression (p=0.001; r=0.773) in EPI-treated group. EPI was able to prevent an increasing of SIRT1 and NF-kB expression in SD model breast cancer that induced with DMBA. There is a positive correlation between SIRT1 and NF-kB expression in EPI-treated SD rats that were induced by DMBA. ABSTRAK Faktor utama penyebab kanker payudara adalah kombinasi faktor endogen dan eksogen. Faktor endogen antara lain adalah ekspresi SIRT1 dan NF-kB dan Faktor eksogen yang digunakan untuk penelitian ini adalah 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3-3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) merupakan turunan isoflavone yang disintesis dari minyak daun cengkeh. Untuk mengkaji pengaruh EPI dengan kelompok Doxorubicin, tetapi lebih rendah daripada kelompok DMBA (p=0.000). Terdapat korelasi positif antara tingkat ekspresi SIRT1 and NF-kB (p=0.001; r=0.773) pada kelompok perlakuan EPI. EPI dapat mencegah peningkatan ekspresi SIRT1 and NF-kB pada model tikus kanker payudara yang diinduksi dengan DMBA. Terdapat korelasi positif antara tingkat ekspresi SIRT1 and NF-kB (p=0.001; r=0.773) pada tikus model yang diberi EPI.
Gout is the result of metabolism in the body whose levels should not be excessive, everyone has uric acid in their body, because every normal metabolism will produce uric acid while the trigger is food factors and other compounds that contain lots of purines. The purpose of this study was to determine the effect of giving Moringa oleifera leaf infusion on blood uric acid levels in mice using 25 mice which were divided into 5 groups, namely the positive control group, the infusion 20%, and the infusion 40%, infusion 60% and negative control with treatment for 3 weeks. Measurement of uric acid levels using an autocheck tool. The results of the measurement of uric acid levels in the infusion group 20% showed an average decrease of 0.16 mg/dl, in the 40% group the infusion showed an average value of 0.62 mg/dl and in the infusion group 60% showed an average value of 0.92 mg/dl. The higher the concentration of Moringa oleifera infusion, the lower the uric acid level.
Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. The objective of this research is to adding diversity of microorganism that can producing cellulose and observe it's both optimum temperature and pH. First step is isolation of bacteria from Watermelon, Papaya, Cantaloupe, Mango, Dragon fruit, and Banana that found in traditional market in Makassar using Hestrin-Schramm (HS) agar. Each colony bacteria have been separated to making pure culture using HS agar then the colony was screening in HS broth to obtain colony bacteria that producing cellulose. Screening test found that 10 isolate that can produce cellulose which is MB-B02, MS-B01, MS-B03, NB-B03, PB-B01, PB-B02, PB-B03, PB-B04, PS-B01, and PS-B03. Then the colony that producing cellulose was optimize its temperature and pH. Cellulose obtained from optimization was measure it's weight to determine optimum temperature and pH. Based from optimization found that 25 0 C was optimum temperature for MS-B01, MS-B03, NB-B03, dan PB-B04, and 37 0 was optimum temperature for MB-B02, PB-B01, PB-B03, PS-B01, dan PS-B03, meanwhile for isolate PB-B02 optimum both in 25 0 C dan 37 0 C dan pH optimum for all isolate is pH 6.
The research aimed to obtain antibacterial activity of isolate fermentate of endophytic fungi of antique spurge stem (Euphorbia antiquorum L.) on Staphylococcus aureus and Escherichia coli by TLC-Bioautography. The methods used in this research were isolation, purification and isolate screening. The screening results of endophytic fungi isolate of antique spurge stem showed the largest inhibitory zone in preventing both bacteria growth using code IFBS-03 and IFBS-06. The chromatogram profile of antibacterial activity isolate fermentate of the codes IFBS-03 and IFBS-06 showed active spots using TLC-Bioautographic method showed that code IFBS-03 isolate obtained Rf 0.87 and 0.27 values, indicating active spots on Staphylococcus aureus and Escherichia coli. The result of code IFBS-06 isolate obtained Rf 0.83 value active spots against Staphylococcus aureus and values Rf 0.83 and 0.27 value on Escherichia coli. Based on isolation and identification some chemical components obtained that dietyl ether ekstract of thin layer cromatography obtained chemicals components flavanoid at Rf 0.83 and 0.27 value with reagent Antimo (III) chloride.
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