Carbazomycins G (I) and H (II), new congeners of the carbazomycin complex, have been isolated from the culture broth of Streptoverticillium ehimense. They have proved to contain a unique quinol moiety in the molecule. Their structures have been elucidated by mass and NMR spectrometries and X-ray crystallographic analysis. Carbazomycin G showed moderate antifungal activity against Trichophyton species.In a previous paper1}, we have reported the isolation of carbazomycins C, D, E and F as the minor components of carbazomycin complex. The structures of carbazomycins C (IV) and D (V) were determined by spectroscopic and chemical means, and in the present paper are confirmed by an X-ray crystallographic analysis of IV.In our successive search for other active substances produced by the same microorganism, we have found two new components named carbazomycins G (I) and H (II) which are shown to possess a unique quinol moiety in the molecule. In this paper, we report the isolation, physico-chemical and antimicrobial properties, and structure elucidation of I and II by mass,^-H and 13C NMRspectrometries and X-ray crystallographic analysis. The confirmation of the structures of IV and V by X-ray crystallography of IV is also reported.Results and Discussion Isolation of Carbazomycins G and H Streptoverticillium ehimense was cultured in the same wayas reported previously0. The broth filtrate was extracted with ethyl acetate and the extract was fractionated by silica gel columnchromatography using the solvent composed of «-hexane and ethyl acetate (7 : 1~1 : 1). The last fraction eluted with «-hexane and ethyl acetate (1 : 1) was concentrated and further purified by repeated preparative TLCon precoated Silica gel plates (Merck Art. No. 5715) developed with «-hexane and ethyl acetate (2 : 3). Under a UV-light (365 nm), I gave strong blue fluorescence and n gave dark yellow one, and both components gave the same brownish purple color on silica gel TLCby heating after spraying with 10 % sulfuric acid.
The research had been conducted about the analysis of antioxidant activity of yodium (Jatropha multifida L.) leaves using cupric ion reducing antioxidant capacity to determine the existance of antioxidant activity and to measure the antioxidant capacity in yodium (Jatropha multifida L.) leaves ethanolic extract using CUPRAC method. It was experimental research using CUPRAC method to prove the existance of antioxidant activity of yodium (Jatropha multifida L.) leaves. It was started by extracting the leaves using maseration using ethanol 96% solvent. The resulted extracts were analyzed quantitatively and qualitatively by adding CuCl2 solution, neucuproin, NH4Ac buffer system which were measured in UV-Visible spectrophotometer and analyzed at a wavelength of 450 nm. Based on data analysis, it was found that the askorbat acid equivalent (AAE) presentage of yodium (Jatropha multifida L.) leaves was 0.7385 ± 0.0521 mg L-1 AA g-1 extract.
Abstract. Our daily life is very often in contact with free radicals which is one of the causes of disease. To neutralize the work of free radicals Antioxidants are needed. Antioxidants are compounds that donate one or more electrons to oxidant compounds. Jackfruit seeds often become waste that is not used by the community. Jackfruit seeds contain chemicals such as flavonoids, saponins and steroids. This study aims to study and determine the antioxidant activity of jackfruit seed extract (Artocarpus heterophyllus Lam.) By the FRAP method (Ferric Reducing Antioxidant Power). The sample was extracted using the maceration method and the extract obtained was analyzed using a UV-Vis spectrophotometer at a wavelength of 716.20 nm using quercetin as a comparison. Based on data analysis, the average value of antioxidant activity of jackfruit seed extract (Artocarpus heterophyllus Lam.) Amounts to 4.8819 mgQE / g extract, meaning that each gram of extract contains 4.8819 mgQE which is in accordance with quercetin standards. This shows that jackfruit seed extract has antioxidant activity which is equivalent to quercetin.Keywords: Antioxidants, Jackfruit Seeds (Artocarpus heterophyllus Lam.), FRAP, UV-Vis Spectrophotometer
Clove is one of the plants that has the potential as an antibacterial. Clove, besides being known as a food flavoring, also has properties as an analgesic, bacteriostatic, and treat stomach cramps. This study aims to isolate and obtain a chromatogram profile of clove flowers (Syzygium aromaticum L.) against bacteria that cause dental caries infection. The search for bioactive compounds in this study was to isolate endophytic fungi in clove flowers and then purified and tested macroscopically. Then a screening test was carried out and then continued for 21 days fermentation using Potato Dextrose Broth medium to separate the supernatant and mycelia, then extracted using ethyl acetate solvents to obtain ethyl acetate extract. Antibacterial activity testing was carried out by TLC-Bioutography method using ethyl acetate: n-hexane (4: 1) eluent. The results of this study obtained five endophytic fungi isolates that were active against the bacteria Streprococcus mutans and Porphyromonas gingivalis. Isolates that have the greatest activity, namely isolates with the code IFBC-01. The results of TLC-Bioautography obtained one speck with a value of rf: 0.67 which means it has antibacterial activity.
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