GSTM1 null genotype increases the risk of pre-eclampsia. Combined GSTT1 and GSTM1 null genotype, the risk was even higher.

Iron deficiency anemia (IDA) and beta-thalassemia trait (BTT) are the common causesof microcytic hypochromic anaemia. Several discrimination indices have beenintroduced to discriminate quickly these similar entities via parameters obtained fromautomated cell counter. The purpose of the study was to compare the value of twodiscrimination indices, red cell distribution width index (RDWI) and red cell distributionwidth (RDW) in differentiation of BTT and IDA. This study consists of 57 cases ofBTT and 72 cases of IDA. Severe anaemia (<7.0 gm/dl) had been excluded becausethese cases are not confused with BTT cases in practice. Sensitivity, specificity,positive and negative predictive values and Youden’s index of both indices indifferentiation of BTT and IDA had been calculated. RDWI appears to be reliable anduseful index for differentiation of iron deficiency anaemia and beta thalassaemia trait.Again RDWI is better than RDW in differentiating BTT from IDA.Key words: Microcytic hypochromic anemia; discrimination indices.DOI: 10.3329/bjch.v33i3.5690Bangladesh Journal of Child Health 2009; Vol.33(3): 100-103

Key words: Beta thalassaemia; discrimination indicesDOI: 10.3329/bjpath.v25i1.4126Bangladesh J Pathol 25(1): 14-17

Worldwide, pre‐eclampsia (PE) ranks as a major obstetric problem leading to substantial maternal and perinatal morbidity and mortality, especially in developing countries. Clinically it appears as a maternal syndrome, including hypertension and proteinuria after second half of pregnancy. PE affected by genetic trait, is associated with oxidative stress due to imbalance between the production of reactive oxygen species and the ability of antioxidant process. The glutathione S‐transferases (GST) are a group of enzymes which are involved in protecting cells from oxidative stress. In our study, we evaluated the risk of developing preeclampsia related to GSTT1 and GSTM1 genotypes. 104 patients having preeclampsia and 200 healthy controls having no history of preeclampsia were recruited for the study after getting written consent from them. DNA was extracted from the blood and Polymerase Chain Reaction (PCR) method was used to identify genotypes of GSTT1 and GSTM1 in study subjects. We used odds ratio to calculate the risk between cases and controls using SPSS 17. Genotype frequency of GSTM1 nul genotype showed significant difference between case and control group (p<0.001). Combined null genotype for GSTT1 and GSTM1 showed significant difference between two groups when compared to combined present genotype for GSTT1 and GSTM1 (p<0.001). There was significant association for GSTM1 null genotype with the development of preeclampsia (p<0.001) and the odds ratio was significantly higher in patients compared to control subjects (OR=4.75; 95% CI=2.17–10.39). For GSTT null genotype, there was increased risk for developing preeclampsia but the association was not significant (OR=1.22; 95% CI=0.58–2.57).In case of GSTT1 and GSTM1 combined genotype, patients having both null genotypes for GSTT1 and GSTM1 showed significance (p<0.001) and higher risk of developing preeclampsia when compared to control subjects (OR=7.64; 95% CI=2.38–24.60). We found a significant association between GSTM1 null genotype and the risk of developing preeclampsia in Bangladeshi population. No risk was found for GSTT1 null genotype and preeclampsia. For combined null genotype of GSTT1 and GSTM1, the risk was even higher relative to the risk of either GSTT1 null or GSTM1 null genotype alone.Support or Funding InformationDear colleague, I hereby, would like to express my motivation with this letter to attend the American Society for Biochemistry and Molecular Biology at San Diego, USA from 21–25 April 2018.The conference topics are relevant to my professional skills, and therefore I would welcome the opportunity to attend this conference having researchers from different countries of the world. I am working as an program coordinator at RHSTEP and at the same working as a predatory PhD student at Department of Biochemistry and Molecular Biology of Dhaka University.As a unique added value of this event, I will find a chance for networking with other scientists from different countries, which is an excellent opportunity for horizontal exchange of knowledge and experience. This networking will expand my opportunities for planning collaborative research projects in future.I have completed my MBBS from Moulana Bhasani Medical College and MPH from American International University, Bangladesh. In case of all three degrees, the medium of instruction was English.Unfortunately I do not have fund to attend this conference and I will apply for a travel grant.My attendance at this training will be very helpful for my carrier development and I would highly appreciate to be one of the selected participants.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Background: Neonatal sepsis is a life-threatening condition with high mortality and morbidity throughout the world. Objective: This study evaluated the diagnostic role of serum procalcitonin (PCT), interleukin-6 (IL-6), tumor necrosis factor-a (TNF-a) and C-reactive protein (CRP) in the early diagnosis of neonatal sepsis. Methods: This cross-sectional study was conducted among neonates admitted to the special baby care unit (SCABU) of BIRDEM General Hospital, Dhaka, from November 2018 to April 2019. According to selection criteria, 90 clinically suspected cases of neonatal sepsis were selected and categorised into confirmed, probable, and no sepsis groups based on CRP, white cell count, platelet count, and blood culture results. Serum PCT, IL-6, and tumor  necrosis factor (TNF-a) were estimated in all cases by standard laboratory methods. Results: Serum PCT, IL-6, CRP, and TNF-awere significantly higher in confirmed and probable sepsis groups in comparison to no sepsis group. Among the studied biomarkers, serum PCT was found most sensitive (95% sensitivity), and serum IL-6 was found most specific biomarkers (65.7% specificity) than CRP and TNF-a for the diagnosis of neonatal septicaemia. Though the accuracy of both PCT and IL-6 was found equal (70%), but the positive predictive value (PPV) and negative predictive value (NPV) of serum PCT were higher than IL-6. Conclusion: Both serum PCT and IL-6 are more sensitive and specific markers than CRP and TNF-a in the diagnosis of neonatal sepsis. Moreover, serum PCT is more useful than IL-6. Bangladesh Med Res Counc Bull 2020; 46(2): 83-89

A N acetyltransferases 2 (NAT2) is one of the phase II metabolizing enzyme that participate in the bioconversion of heterocyclic arylamines into electrophilic nitrenium ions, which are important ultimate carcinogens that are directly implicated in tumor initiation process. Prostate epithelial cell express N acetyltransferases (NAT) enzymes and recent molecular epidemiological studies have analyzed the relationship between NAT2 in etiology of prostate cancer. A review by chen (2001) in prostate cancer (PCa) suggests that the frequencies of some polymorphisms in certain genes differ among different racial and ethnic groups. In a case control study in India by Srivastava and Mittal (2005), observed significant association between rapid acetylator genotype NAT2 and PCa in tobacco users (OR = 3.43, 95% CI: 1.68-7.02, p< 0.001) when compared with controls. Hamasaki et al (2003) in Japanese men, observed the frequency of the NAT slow acetylator genotype was statistically higher among prostate cancer patients (17.1%) compared with controls (8.6%). Another case control study in Turkey by Kosova et al. (2009), concluded that Nat2* 6A and NAT2 *7A/B gene polymorphism were significantly associated with prostate cancer. DOI: http://dx.doi.org/10.3329/akmmcj.v5i2.21131 Anwer Khan Modern Medical College Journal Vol. 5, No. 2: July 2014, Pages 39-42

Background: Type 2 diabetes mellitus is a chronic disease characterized by relative or absolute deficiency of insulin, resulting in glucose intolerance.Objectives: The present study was planned to see the associations of serum uric acid with positive Rheumatoid factor in type 2 male diabetes mellitus patients. Methodology: This case control study was carried out at the department of Biochemistry at Ibrahim Medical College, Dhaka, Bangladesh. The duration of the study was from June 2015 to June 2016 for a period of one year. In this present study, male patients with type 2 diabetes mellitus were taken as case group and age and sex matched healthy male were taken as control group. Rheumatoid factor was measured from the blood of all case and control group respondents. Others blood para meters were also measured for the correlation with the diabetes mellitus patients.Results: In this present study, 110 male patients presented with type 2 diabetes mellitus were recruited as case and age and sex matched healthy male were recruited as control. More rheumatoid factor positive in type 2 DM male patients with the uric acid range between 6.5 to 9.5 mg/dL. The number of patients was 5 out of total 9 rheumatoid factor positive cases. In this study serum uric acid was significantly correlated with rheumatoid factor in type 2 male diabetic patients. Rheumatoid factor positive cases were taking insulin among 9 and it was statistically significantly associated (p<0.001). Conclusion: In this study serum uric acid is significantly associated with positive rheumatoid factor in type 2 male diabetic patients.Journal of Current and Advance Medical Research 2017;4(2):58-62

Type 1 diabetes mellitus is one of the most common endocrine and metabolic disorder in children and adolescents. Alteration of serum magnesium level may be associated with metabolic control and diabetic complications. The aim of the study was to evaluate serum magnesium level and find out its relationship with glycemic control in type 1 diabetic children and adolescents. For this study 80 type 1 diabetic children & adolescents with age range 1 to 18 years and 80 aged matched healthy controls were enrolled from the outpatient department of Changing Diabetes in Children, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine & Metabolic disorders, General Hospital, Dhaka. Using a cross sectional design, we measured anthropometric parameters and serum magnesium level of all study subjects. We also estimate the fasting plasma glucose and HbA1c levels of the diabetic children and adolescents and controls. The mean ± SD of serum magnesium level was significantly lower in patient with type 1 DM compared to control (0.7 ± 0.1 vs 0.8 ± 0.1 mmol/L respectively; p= < 0.001). Lower level of magnesium was found in subjects with poor glycemic control compared to good glycemic control (0.6 ± 0.1 vs 0.8 ± 0.1 mmol/L respectively; p= < 0.001). This study showed that serum magnesium level was lowered  in type 1 diabetic children and adolescents and it was strongly associated with poor glycemic control which potentially contributing to the early development of diabetic complications. Bangladesh Med J. 2019 Sep; 48 (3): 16-20