A validated method was developed for determination of diclofenac sodium, considered a model hydrophilic drug for in vitro permeation studies, in Eagle's Minimum Essential Medium (MEM) with Earle's balanced salt solution. Liquid chromatography was used to determine diclofenac sodium. This method was developed with a reversed-phase column Supercosil LC 18, DB 25 cm × 4.5 mm; the mobile phase was methanol with 3% (v/v) acetic acid–Milli-Q water (74 + 26), and detection was at 283 nm. The detection and quantitation limits were 2.41 × 10–8 and 3.31 × 10–5 μg/μL, respectively. The accuracy within-day (n = 3) and day-to-day (n = 7) was 98.83%; the mean variation coefficient for inter- (n = 7) and intraday precision (n = 3) was 12.20%, thus, not exceeding 15%. This method can be used as an analytical procedure for the determination of diclofenac sodium in MEM for in vitro permeation studies.
Recently, there has been an interest in the use of shed snake skin as alternative model biomembrane for human stratum corneum. This research work presented as objective the qualitative characterization of alternative model biomembranes from Bothrops jararaca and Spilotis pullatus by FT-Raman, PAS-FTIR and DSC. The employed biophysical techniques permitted the characterization of the biomembranes from shed snake skin of B. jararaca and S. pullatus by the identification of vibrational frequencies and endothermic transitions that are similar to those of the human stratum corneum.Uniterms: Biomembrane/alternative models. Bothrops jararaca/skin/qualitative characterization. Spilotis pullatus/skin/qualitative characterization. Human stratum corneum/similars.Existe atualmente interesse no uso da muda de pele de cobra como modelos alternativos de biomembranas da pele humana. O presente trabalho apresentou como objetivo a caracterização qualitativa de modelos alternativos de biomembranas provenientes de mudas de pele de cobra da Bothrops jararaca e Spilotis pullatus por espectroscopia Raman (FT-Raman), espectroscopia fotoacústica no infravermelho (PAS-FTIR) e calorimetria exploratória diferencial (DSC). As técnicas biofísicas FT-Raman, PAS-FTIR e DSC permitiram caracterizar qualitativamente os modelos alternativos de biomembranas provenientes das mudas de pele de cobra da B. jararaca e S. pullatus e identificar freqüências vibracionais e transições endotérmicas similares ao estrato córneo humano.Unitermos: Biomembranas/modelos alternativos. Bothrops jararaca/pele/caracterização qualitativa. Spilotis pullatus/pele/caracterização qualitativa. Estrato córneo humano/similares.
Avaliação da influência de tensoativos na pele de muda de cobra (Bothrops jararaca e Spilotis pullatus) por espectroscopia fotoacústica no infravermelho, calorimetria exploratória diferencial e espectroscopia Raman Comissão julgadora da Dissertação para a obtenção do grau de Mestre
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