Spectroscopic studies of stratum corneum model membrane from Bothrops jararaca treated with cationic surfactant

Baby, André Rolim; Lacerda, Aurea Cristina Lemos; Velasco, Maria Valéria Robles; Lopes, Patrícia Santos; Kawano, Yoshio; Kaneko, Telma Mary

doi:10.1016/j.colsurfb.2006.04.005

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…22,23 In few cases a tape stripping method was applied to remove the superficial cells of snake scales. 31 In the present study, the inner and outer surface of scales have been singularly analyzed by FTIR and Raman microscopy, and the results fit with the previous studies. The outer scale surface of both species here analyzed contains protein mainly with b-sheet conformation (amide bands, b-sheet) and lipids (bands in the range 3000-2800 cm 21 ) (Figures 2-4).…”

Section: Discussionsupporting

confidence: 76%

Keratin‐lipid structural organization in the corneous layer of snake

et al. 2009

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The shed epidermis (molt) of snakes comprises four distinct layers. The upper two layers, here considered as beta-layer, contain essentially beta-keratin. The following layer, known as mesos-layer, is similar to the human stratum corneum, and is formed by thin cells surrounded by intercellular lipids. The latter layer mainly contains alpha-keratin. In this study, the molecular assemblies of proteins and lipids contained in these layers have been analyzed in the scale of two species of snakes, the elapid Tiger snake (TS, Notechis scutatus) and the viperid Gabon viper (GV, Bitis gabonica). Scanning X-ray micro-diffraction, FTIR and Raman spectroscopies, thermal analysis, and scanning electron microscopy experiments confirm the presence of the three layers in the GV skin scale. Conversely, in the TS molt a typical alpha-keratin layer appears to be absent. In the latter, experimental data suggest the presence of two domains similar to those found in the lipid intercellular matrix of stratum corneum. X-ray diffraction data also allow to determine the relative orientation of keratins and lipids. The keratin fibrils are randomly oriented inside the layers parallel to the surface of scales while the lipids are organized in lamellar structures having aliphatic chains normal to the scale surface. The high ordered lipid organization in the mature mesos layer probably increases its effectiveness in limiting water-loss.

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Section: Discussionsupporting

confidence: 76%

Keratin‐lipid structural organization in the corneous layer of snake

et al. 2009

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“…A rubber composite was used as standard. The experimental conditions were: (1) resolution: 4 cm -1 ; (2) slit: 10; (3) gain: 4; and (4) speed of the mobile mirror: 0.05 cm/s (Baby et al, 2007;Baby et al, 2006a). The measurements were done using three replicates.…”

Section: Pas-ftirmentioning

confidence: 99%

“…Among these methods: X-ray diffraction, electron paramagnetic resonance, nuclear magnetic resonance, DSC (Differential Scanning Calorimetry), FT-Raman, and PAS-FTIR (Lafleur, 2001;Baby et al, 2006a;Baby et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Baby

Lacerda

Sarruf

et al. 2009

Braz. J. Pharm. Sci.

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Recently, there has been an interest in the use of shed snake skin as alternative model biomembrane for human stratum corneum. This research work presented as objective the qualitative characterization of alternative model biomembranes from Bothrops jararaca and Spilotis pullatus by FT-Raman, PAS-FTIR and DSC. The employed biophysical techniques permitted the characterization of the biomembranes from shed snake skin of B. jararaca and S. pullatus by the identification of vibrational frequencies and endothermic transitions that are similar to those of the human stratum corneum.Uniterms: Biomembrane/alternative models. Bothrops jararaca/skin/qualitative characterization. Spilotis pullatus/skin/qualitative characterization. Human stratum corneum/similars.Existe atualmente interesse no uso da muda de pele de cobra como modelos alternativos de biomembranas da pele humana. O presente trabalho apresentou como objetivo a caracterização qualitativa de modelos alternativos de biomembranas provenientes de mudas de pele de cobra da Bothrops jararaca e Spilotis pullatus por espectroscopia Raman (FT-Raman), espectroscopia fotoacústica no infravermelho (PAS-FTIR) e calorimetria exploratória diferencial (DSC). As técnicas biofísicas FT-Raman, PAS-FTIR e DSC permitiram caracterizar qualitativamente os modelos alternativos de biomembranas provenientes das mudas de pele de cobra da B. jararaca e S. pullatus e identificar freqüências vibracionais e transições endotérmicas similares ao estrato córneo humano.Unitermos: Biomembranas/modelos alternativos. Bothrops jararaca/pele/caracterização qualitativa. Spilotis pullatus/pele/caracterização qualitativa. Estrato córneo humano/similares.

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“…12,13) Shed snake skin consists of three distinctive layers and the main barrier to water permeation resides in the mesos layer of the epidermis, which contains the extremely flat and thin cells surrounded by intercellular lipids, similar to the human SC. 9,10,14) The potential of shed snake skin as a model biological membrane for transdermal research has been reported. [8][9][10] Therefore, we have used shed snake skin for our studies.…”

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confidence: 99%

Evaluation of the Mechanism of Skin Enhancing Surfactants on the Biomembrane of Shed Snake Skin

Wonglertnirant

Ngawhirunpat

Kumpugdee-Vollrath

2012

Biological & Pharmaceutical Bulletin

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The aim of the present work was to investigate the effects of different surfactants at various concentrations as a skin penetration enhancer through the biomembrane of the shed skin of Naja kaouthia. Additionally, the enhancer mechanism(s) of each class of surfactants were evaluated using physical characterization techniques, such as scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and small and wide angle X-ray scattering (SWAXS). Our results showed that skin permeability increased with increasing concentrations of surfactants and the degree of increase was higher for the model hydrophilic permeant, deuterium dioxide (D 2 O), than the lipophilic permeant, ketoprofen (KP). Ionic surfactants, sodium lauryl sulfate (SLS) and cetyl trimethyl ammonium bromide (CTAB), demonstrated higher enhancement ability than the polyoxyethylene (20) sorbitan mono-oleate (Tween 80) non-ionic surfactant, which was consistent with the results from physical characterization studies. Increasing amounts of permeated drug resulted in an increase in membrane interactions. From our observations, it can be assumed that SLS and CTAB can be localized inside the biomembrane and thereby enhance drug permeation mainly through interactions with intercellular lipids in the stratum corneum (SC) and the creation of a perturbed microenvironment among lipid alkyl chains and polar head groups.Key words surfactant; shed snake skin; enhancer mechanism; permeation; attenuated total reflectance-Fourier transform infrared spectroscopy; small-wide angle X-ray scattering The barrier function of the skin resides in the stratum corneum (SC), the outermost layer of the skin. Its multilayered wall-like structure contains terminally differentiated keratin-rich epidermal cells that are embedded in an intercellular lipid-rich matrix.

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Spectroscopic studies of stratum corneum model membrane from Bothrops jararaca treated with cationic surfactant

Cited by 10 publications

References 14 publications

Keratin‐lipid structural organization in the corneous layer of snake

Keratin‐lipid structural organization in the corneous layer of snake

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Evaluation of the Mechanism of Skin Enhancing Surfactants on the Biomembrane of Shed Snake Skin

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