Evaluation of the interaction of surfactants with stratum corneum model membrane from Bothrops jararaca by DSC

Baby, André Rolim; Lacerda, Aurea Cristina Lemos; Velasco, Maria Valéria Robles; Lopes, Patrícia Santos; Kawano, Yoshio; Kaneko, Telma Mary

doi:10.1016/j.ijpharm.2006.04.006

Cited by 13 publications

(14 citation statements)

References 14 publications

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“…The phase transition of the lipid bilayer from lamellar gel to liquid state is attributed to the temperature of 75-85 °C. The transition that occurs at 105 °C represents the dehydration and denaturation of the protein fraction of the SC and the presence of a certain amount of water in the sample is required for its detection by the technique (Baby et al, 2006b;Golden et al, 1986, Ashton et al, 1992Leopold, Lipppold, 1995).…”

Section: Resultsmentioning

confidence: 99%

“…The samples were immersed in distilled water for eight hours in order to hydrate. After samples' hydration, the excess of water was removed with smooth compression using quantitative filter paper (Baby et al, 2006b). The samples were kept between two microscopy laminas and maintained in a desiccator until the time of analysis with FT-Raman, PAS-FTIR and DSC.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The samples were put in the analytical compartment and 256 co-additions corresponding to the spectra between 3500 and 200 cm -1 were obtained. The conditions were the following: (1) laser potency: 250 mW; (2) resolution: 4 cm -1 ; (3) slit: 7; and (4) gain: 4 (Baby et al, 2007;Baby et al, 2006b). Three replicates were used.…”

Section: Ft-ramanmentioning

confidence: 99%

“…Samples of approximately 2.0 mg were sealed in a semi-hermetic crucible and were submitted to the following conditions (Baby et al, 2006b) in the DSC 10 equipment: (1) initial temperature: 25.0 °C; (2) cooling ramp: 20.0 °C/min to 0 °C (3) isotherm: 5 min (4) heating ramp: 10.0 °C/min to 160 °C (5) cooling ramp: 20.0 °C/min to 0 °C (6 ) isotherm: 5 min (7) heating ramp: 10.0 °C/min to 160 °C; and (8) final temperature: 25.0 °C (Baby et al, 2006b).…”

Section: Dscmentioning

confidence: 99%

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Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Baby

Lacerda

Sarruf

et al. 2009

Braz. J. Pharm. Sci.

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Recently, there has been an interest in the use of shed snake skin as alternative model biomembrane for human stratum corneum. This research work presented as objective the qualitative characterization of alternative model biomembranes from Bothrops jararaca and Spilotis pullatus by FT-Raman, PAS-FTIR and DSC. The employed biophysical techniques permitted the characterization of the biomembranes from shed snake skin of B. jararaca and S. pullatus by the identification of vibrational frequencies and endothermic transitions that are similar to those of the human stratum corneum.Uniterms: Biomembrane/alternative models. Bothrops jararaca/skin/qualitative characterization. Spilotis pullatus/skin/qualitative characterization. Human stratum corneum/similars.Existe atualmente interesse no uso da muda de pele de cobra como modelos alternativos de biomembranas da pele humana. O presente trabalho apresentou como objetivo a caracterização qualitativa de modelos alternativos de biomembranas provenientes de mudas de pele de cobra da Bothrops jararaca e Spilotis pullatus por espectroscopia Raman (FT-Raman), espectroscopia fotoacústica no infravermelho (PAS-FTIR) e calorimetria exploratória diferencial (DSC). As técnicas biofísicas FT-Raman, PAS-FTIR e DSC permitiram caracterizar qualitativamente os modelos alternativos de biomembranas provenientes das mudas de pele de cobra da B. jararaca e S. pullatus e identificar freqüências vibracionais e transições endotérmicas similares ao estrato córneo humano.Unitermos: Biomembranas/modelos alternativos. Bothrops jararaca/pele/caracterização qualitativa. Spilotis pullatus/pele/caracterização qualitativa. Estrato córneo humano/similares.

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Section: Resultsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Ft-ramanmentioning

confidence: 99%

Section: Dscmentioning

confidence: 99%

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Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Baby

Lacerda

Sarruf

et al. 2009

Braz. J. Pharm. Sci.

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“…12,13) Shed snake skin consists of three distinctive layers and the main barrier to water permeation resides in the mesos layer of the epidermis, which contains the extremely flat and thin cells surrounded by intercellular lipids, similar to the human SC. 9,10,14) The potential of shed snake skin as a model biological membrane for transdermal research has been reported.…”

mentioning

confidence: 99%

Evaluation of the Mechanism of Skin Enhancing Surfactants on the Biomembrane of Shed Snake Skin

Wonglertnirant

Ngawhirunpat

Kumpugdee-Vollrath

2012

Biological & Pharmaceutical Bulletin

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The aim of the present work was to investigate the effects of different surfactants at various concentrations as a skin penetration enhancer through the biomembrane of the shed skin of Naja kaouthia. Additionally, the enhancer mechanism(s) of each class of surfactants were evaluated using physical characterization techniques, such as scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and small and wide angle X-ray scattering (SWAXS). Our results showed that skin permeability increased with increasing concentrations of surfactants and the degree of increase was higher for the model hydrophilic permeant, deuterium dioxide (D 2 O), than the lipophilic permeant, ketoprofen (KP). Ionic surfactants, sodium lauryl sulfate (SLS) and cetyl trimethyl ammonium bromide (CTAB), demonstrated higher enhancement ability than the polyoxyethylene (20) sorbitan mono-oleate (Tween 80) non-ionic surfactant, which was consistent with the results from physical characterization studies. Increasing amounts of permeated drug resulted in an increase in membrane interactions. From our observations, it can be assumed that SLS and CTAB can be localized inside the biomembrane and thereby enhance drug permeation mainly through interactions with intercellular lipids in the stratum corneum (SC) and the creation of a perturbed microenvironment among lipid alkyl chains and polar head groups.Key words surfactant; shed snake skin; enhancer mechanism; permeation; attenuated total reflectance-Fourier transform infrared spectroscopy; small-wide angle X-ray scattering The barrier function of the skin resides in the stratum corneum (SC), the outermost layer of the skin. Its multilayered wall-like structure contains terminally differentiated keratin-rich epidermal cells that are embedded in an intercellular lipid-rich matrix.

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In Vitro, Ex Vivo, and In Vivo Evaluation of the Effect of Saturated Fat Acid Chain Length on the Transdermal Behavior of Ibuprofen-Loaded Microemulsions

Ren

Deng

Meng

et al. 2014

Journal of Pharmaceutical Sciences

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Evaluation of the interaction of surfactants with stratum corneum model membrane from Bothrops jararaca by DSC

Cited by 13 publications

References 14 publications

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Spectroscopic and thermal characterization of alternative model biomembranes from shed skins of Bothrops jararaca and Spilotis pullatus

Evaluation of the Mechanism of Skin Enhancing Surfactants on the Biomembrane of Shed Snake Skin

In Vitro, Ex Vivo, and In Vivo Evaluation of the Effect of Saturated Fat Acid Chain Length on the Transdermal Behavior of Ibuprofen-Loaded Microemulsions

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