Abstrak. Telah dilakukan penelitian mengenai estimasi pertumbuhan larva lalat Black Soldier (Hermetia illucens) (WRI). The method in this study, rice straw fermented by P.chrysosporium (4%), than substrat used for larvae fed.Two hundreds 6-day-old larvae were fed on rice straw with variation of 12.5, 25, 50, and 200 mg/larva/day, replicated three times, until (12,96 %) and reduction index (0,42), development time to pupation 39 day, and feeding duration recorded on feeding 100 mg/larva/day. Based on this study, it concluded best feeding rate for growth of BSF larvae is 100 mg/larva/hari.

<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 ⁰C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24^th, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., &amp; Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 314-320. </p>

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In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.

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Black Soldier fly larvae is an insect capable to convert organic waste into body biomass. The purpose of this research was to determine the productivity, mass balance of the consumption process, and content of protein and fat of prepupa which has a potency as a livestock feed. This research used 6-day-old larvae, they were fed with cassava skin that has been smoothed with a variety of feeding doses: 12.5; 25; 50; 100; and 200 (mg / larvae / day) with ratio of feed and water 2: 1. The results showed that the feeding of 200 mg / larva / day produced the best larval growth with biomass productivity of 1.54 mg / day, residue yielded of 67.1 mg / day. The organic waste was convertion into larvae biomass. In this study, approximately consumption process scale is feed successfully converted to biomass 2.77%, metabolism 6.98%, and 90.24% residue. Protein content is 25.7%. It can be concluded that the larvae are able to convert organic waste into high protein biomass and potentially being used as a livestock feed. This research acts a model to predict mass balance of substrates for feed. The benefit of this research is the science approach used in this study that can be used as a reference by researchers and the community to determine the biomass production of an organism.

Wild species of begonia existed in remnant forest of Cibodas Botanic Gardens, but their diversity, distribution and ecological preference were not much known. Data of condition and habitat characteristic of wild begonia are important in their cultivation. The aims of this study are to inventory the diversity of wild begonias and their distribution habitat preference in remnant forest of Cibodas Botanic Gardens. Sample were collected from four remnant forest using exploration method. Analysis of vegetation where wild Begonia existed was conducted using 1x1 m plot in around every individu of Begonia found. The principle component analysis (PCA) was carried out using R program version 2.11.0. The result showed that 50% of the mountain Begonia in West Java can be found in remnat forest area. The light intencity, soil humidity and air humidity were highly correlated to distribution of wild Begonia. The microclimate conditions in remnant forest area are appropriate with habitat of Begonia.

Cibodas Botanic Garden, as ex situ conservation areas, preserves living materials ofthe wet mountains flora. Recently, bioprospecting study for dye in this areas has not yetbeen done. Therefor, the aim of this study was to inventory of natural dye in Cibodas BotanicGarden. Sampel were collected by exploration methods. Parts of plants, kinds of color andits chemical compound were recorded based on observation, interview with nurseries andliterature study. A total of 122 species that belongs to 57 families were potentially source asnatural dye, 23 of them has been traditionally used as natural dye in the various places inIndonesia. The plant family of Fabaceae, Rosaceae, Symplocaceae, and Myrtaceae are themost families that used as a source of dye. The color can be produce are ranging from red,purple, yellow, black, brown, green, blue, and orange. The plant organs used as a source ofdye are roots, stems, leaves, flowers, and fruit. Begonia semperflorens,Berberis nepaulensis,B.fortunei, Symplocosspp., Liquidambar formosana, andDiospyros kaki were potentially fornatural dye, as reported from other countries.