In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar, Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.
Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been known and used as medicines. Exploration on torch ginger plant needs to be done to conservate and determine genetic diversity. Study of genetic diversity torch ginger in the district of Pangandaran is important because as long as there has been no study in depth the potential of genetic diversity and phylogenetic relationship germplasm of torch ginger Genetic diversity based on this research had a proportion of 47% of the total of 100% in the PC1-PC3 was based on the analysis Principal Component Analysis (PCA) with the character influencing that form clumps, line leaf midrib, plant height, stem diameter, leaf length, color scales rhizome, stem color, rhizome and the leaf base forms. Based on the results of the genetic diversity cluster analysis found that narrow torch ginger euclidean indicated by coefficient of 0.79 and was divided into six clusters. Accession which had the closest kinship i.e. accession N1 from Cikadu subdistrict of Cintakarya and accession N2 from Cikoang subdistrict of Cintakarya, while accession with the farthest kinship namely accession N29 from Jangraga subdistrict of Mangunjaya with range euclidean of 0.79.
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