We studied the effect of the combined action of ionizing radiation and induced immobilization stress on the lipid peroxidation process and antioxidant protection of organs (mesenteric lymph nodes, spleen, adrenal glands, thymus, and liver) and immune cels - the blood lymphocytes. Results were obtained on the role of free-radical oxidation in combination with exposure to ionizing radiation and immobilization stress at an early stage in the experiment. Gamma radiation in the acute period resulted in significant changes in lipoperoxidation and antioxidant systems. The first period of immobilization stress was marked by the imbalance of LPO-AOS systems disturbance with an accumulation of toxic compounds in tissues which had affected their function. The combined sublethal gamma radiation and immobilization stress disturbed the functional activity of adaptive systems of the body in the early stage of adaptation syndrome. Furthermore, the results show the dominant role of ionizing radiation in it.
BACKGROUND: For many years, the world community has been concerned with the problem of the consequences of radiation exposure on the human body. A wide range of possible variants of radiation effects on humans and biota determines the range of necessary pharmacological means of protecting the organism and populations. In the mechanisms of the formation of radioresistance, the leading role is assigned to the processes of lipid peroxidation (LPO) and the antioxidant (AO) system. The study of the effect of such factors as radiation and immobilization stress on the body separately and in combination is relevant since the modern conditions of human habitation are characterized by high urbanization, physical inactivity, and a complex radioecological situation in a number of regions. AIM: The aim of the study was to study the role of free radical oxidation in the tissues of the adrenal glands and immunocompetent organs and cells under the combined effect of a sublethal dose of gamma radiation and immobilization stress in the experiment. MATERIALS AND METHODS: The work was carried out on 40 male Wistar rats: I-control; II-subjected to immobilization stress after 1 h; III-exposed to gamma irradiation; and IV-tested combined effects (immobilization stress and gamma radiation). Before the exposure, there was topometric-dosimetric preparation of the experimental animals. To this end, the object was placed on an isocentric therapeutic desk of Terasix X-ray simulator (Czech Republic), which is similar to the therapeutic desk of the γ-irradiator by its construction and parameters. RESULTS: The results obtained make it possible to assess the role of free radical oxidation under the combined action of ionizing radiation at a sublethal dose and immobilization stress in the experiment. The combined effect was accompanied by the accumulation of diene conjugates and malondialdehyde products in homogenates and the development of double oxidative stress in the test objects. The dominant role of ionizing radiation was revealed under the combined effects of immobilization stress and radiation factor. CONCLUSIONS: In animals of the 4th group, the state of oxidative-metabolic processes was characterized by overproduction of LPO products and subsequently by depression of AO defense. The urgency of continuing research on the combined effects of radiation and stress factors on public health and taking measures to eliminate negative effects on the population seems to be undoubted.
BACKGROUND:Extreme environmental situation in the Aral crisis has caused a massive chemical pollution of the territory for decades with high doses of pesticides, herbicides. Discharge of industrial waste into the rivers that feed the Aral Sea has lead to the development of various pathological processes in the human body, as well as disruption of reproductive function in young men.AIM:To evaluate the performance of molecular cellular changes in the sperm of men under the influence of dust and salt aerosols in Aral Sea region.MATERIAL AND METHODS:Clinical and laboratory studies were conducted in men 5 settlements (Aralsk-city, v. Aiteke-Bi, v. Zhalagash, v. Zhusaly, v. Shieli). We have studied male ejaculate obtained after 4-5 days of abstinence, and placed it in a warm tube with a glass stopper. On the investigation proceeded ejaculate within 20-30 minutes after its preparation, during which time he was subjected to liquefaction. Isolation and quantification of ASF, RNA, DNA, and determining the fraction of histones in sperm was performed by the method of Markusheva and Savina.RESULTS:It was found that the value of ASF in the semen of men living in the zone of ecological disaster higher compared with the values of parameters in men living in the area of environmental crisis, and this trend is observed in all age groups. The study of circulating extracellular DNA and RNA in the sperm of men registered their decline with a corresponding increase of acid precursors that can be attributed to the degradation of nucleic acids under the influence of negative factors in the complex area of ecological trouble. Also, according to a study in men residing in the areas of environmental catastrophe at the age of 18-29 years, found an increased content of the H1 histone H2A lower total fraction, H3, H4 - and a sharp increase in histone H2B content - histones.CONCLUSIONS:Men living in environmentally disadvantaged areas of Kyzylorda region under the influence of dust and salt aerosols and other toxicants leads to disruption of the reproductive function in men.
AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H2O. RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups. CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.
This corrects the article on p. 927 in vol. 15, PMID: 36188645.
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