The The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea

Abstract

AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region.
 METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H2O.
 RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups.
 CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.

The Impact of Dust and Salt Aerosols of the Aral Sea on the Tissues of Rat Testes in the Experiment

“…The negative impact of the environment under the conditions of a massive technogenic load is reflected in the deterioration of demographic indicators, a decrease in the functional capabilities and body defenses, and an increase in the morbidity and mortality of the population of the region [1], [2], [3], [4]. A significant increase in the content of extracellular nucleic acids in the blood and ejaculate of men [5] and DNA oxidation products in the blood of women [6] living in the Aral region was revealed.…”