Evaluation of Lipid Peroxidation under Immobilization Stress in Irradiated Animals in Experiment

Abstract

BACKGROUND: For many years, the world community has been concerned with the problem of the consequences of radiation exposure on the human body. A wide range of possible variants of radiation effects on humans and biota determines the range of necessary pharmacological means of protecting the organism and populations. In the mechanisms of the formation of radioresistance, the leading role is assigned to the processes of lipid peroxidation (LPO) and the antioxidant (AO) system. The study of the effect of such factors as radiation and immobilization stress on the body separately and in combination is relevant since the modern conditions of human habitation are characterized by high urbanization, physical inactivity, and a complex radioecological situation in a number of regions. AIM: The aim of the study was to study the role of free radical oxidation in the tissues of the adrenal glands and immunocompetent organs and cells under the combined effect of a sublethal dose of gamma radiation and immobilization stress in the experiment. MATERIALS AND METHODS: The work was carried out on 40 male Wistar rats: I-control; II-subjected to immobilization stress after 1 h; III-exposed to gamma irradiation; and IV-tested combined effects (immobilization stress and gamma radiation). Before the exposure, there was topometric-dosimetric preparation of the experimental animals. To this end, the object was placed on an isocentric therapeutic desk of Terasix X-ray simulator (Czech Republic), which is similar to the therapeutic desk of the γ-irradiator by its construction and parameters. RESULTS: The results obtained make it possible to assess the role of free radical oxidation under the combined action of ionizing radiation at a sublethal dose and immobilization stress in the experiment. The combined effect was accompanied by the accumulation of diene conjugates and malondialdehyde products in homogenates and the development of double oxidative stress in the test objects. The dominant role of ionizing radiation was revealed under the combined effects of immobilization stress and radiation factor. CONCLUSIONS: In animals of the 4th group, the state of oxidative-metabolic processes was characterized by overproduction of LPO products and subsequently by depression of AO defense. The urgency of continuing research on the combined effects of radiation and stress factors on public health and taking measures to eliminate negative effects on the population seems to be undoubted.

Comorbid Conditions in Persons Exposed to Ionizing Radiation and Veterans of the Soviet–Afghan War: A Cohort Study in Kazakhstan

“…In some instances, strokes developed without arterial HT (17.1%). This perspective is also supported by other researchers [ 25 , 26 ].…”

Pathogenetic Mechanisms of Relationship of Metabolic and Morphofunctional Disorders of Thyroid and Adrenal Glands in Diabetes Mellitus and Obesity

“…The lower results were recorded in patients of DM2IHDn and Lipid metabolism in patients with DM1 was manifested by impaired lipogenesis and lipolysis, which was expressed in a significant (p < 0.01) increase of TGs level -in 1.33 times, atherogenic index (AI) -in 1.88 times, and total cholesterol (TC) -in 1.92 times. The level of TGs and atherogenic index (p < 0.001) in patients with DM2o increased in 1.8 times, levels of free fatty acids (FFA), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and apoB -in 2 times [19], [20], [21], [22]. In DM2IHDo patients, expressed disorders were manifested in a significant (p < 0.001) increase in the level of TGs in 1.96 times and atherogenic index in 2 times (Figure 2).…”

Oxidation reactions of cyclopent-2-en-1-yl thiophene derivatives

“…The research team of M. Majdecki et al (21) considered the possibility of using a natural extract from the hin tree as a catalyst. This solution is quite effective for improving the environmental friendliness of oxidation processes (22) , but in the framework of this research, it can cause several experimental difficulties during the purification and analysis of target products. Considering all the above factors, m-chloro-nonbenzoic acid was chosen as an oxidant for this research.…”