Objectives: To compare the interobserver agreement between visual and photogrammetry postural assessment and to determine whether the quantitative photogrammetry results correspond to the symmetries and asymmetries detected through qualitative visual postural assessment. Methods: Twenty-one volunteers (mean age 24±1.9 years) were visually evaluated by three experienced physical therapists, who completed a postural assessment form. The participants' face and whole body were then photographed in the anterior and posterior frontal and sagittal planes. The photographs were used to draw angles from markers fixed to the skin at various anatomical points that are frequent references in traditional postural assessment. These photographs were analyzed by three examiners (other than the ones who performed the visual assessment). The agreement in each postural assessment method was determined using Cramer's V or the Phi coefficient, with the significance level set at 5%. Results: There was agreement between the examiners who used photogrammetry, for all segments analyzed. No agreement was found for the labial commissure (p=0.00), acromioclavicular joint (p=0.01), sternoclavicular joint (p=0.00), anterior and posterior iliac spines (p=0.00 and p=0.01) or inferior angle of the scapula (p=0.00) when assessed visually. The comparison between photogrammetry and visual postural assessment showed that the agreement level between the two assessment methods was poor for some segments of the lower limb and pelvis. Conclusions: Under these experimental conditions, the photogrammetry data were not correlated with the results from the visual postural assessment. The visual postural assessment produced data that were in less agreement than the photogrammetry data, and its use as a gold standard must be questioned.Key words: photogrammetry; posture; assessment; validity; physical therapy. ResumoObjetivos: Comparar a concordância interobservador da avaliação postural visual e por fotogrametria e verificar se os resultados quantitativos da fotogrametria correspondem à detecção de simetrias e assimetrias pela avaliação postural visual qualitativa. Métodos: Vinte e um voluntários (24±1,9 anos) foram inicialmente avaliados visualmente por três fisioterapeutas experientes que preencheram um protocolo de avaliação postural. Em seguida tiveram fotografados a face e o corpo todo nos planos frontal anterior, posterior e sagital. As fotos foram utilizadas para traçar ângulos a partir de marcadores fixados à pele, em vários pontos anatômicos, que são referências frequentes na avaliação postural tradicional. Essas fotografias foram analisadas por três examinadores diferentes da avaliação postural visual. A concordância de cada método de avaliação postural foi avaliada pelos Coeficientes de Cramer V ou de PHI, considerando-se um nível de significância de 5%.Resultados: Foi encontrada uma concordância entre os examinadores que utilizaram a fotogrametria para todos os segmentos avaliados.Não apresentaram concordância os segmentos comissura labia...

Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.

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We sought to investigate the prevalence of potentially pathogenic bacteria in secretions and tonsillar tissues of children with chronic adenotonsillitis hypertrophy compared to controls. Prospective case-control study comparing patients between 2 and 12 years old who underwent adenotonsillectomy due to chronic adenotonsillar hypertrophy to children without disease. We compared detection of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Pseudomonas aeruginosa, and Moraxella catarrhalis by real-time PCR in palatine tonsils, adenoids, and nasopharyngeal washes obtained from 37 children with and 14 without adenotonsillar hypertrophy. We found high frequency (>50%) of Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, and Pseudomonas aeruginosa in both groups of patients. Although different sampling sites can be infected with more than one bacterium and some bacteria can be detected in different tissues in the same patient, adenoids, palatine tonsils, and nasopharyngeal washes were not uniformly infected by the same bacteria. Adenoids and palatine tonsils of patients with severe adenotonsillar hypertrophy had higher rates of bacterial coinfection. There was good correlation of detection of Moraxella catarrhalis in different sampling sites in patients with more severe tonsillar hypertrophy, suggesting that Moraxella catarrhalis may be associated with the development of more severe hypertrophy, that inflammatory conditions favor colonization by this agent. Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis are frequently detected in palatine tonsils, adenoids, and nasopharyngeal washes in children. Simultaneous detection of Moraxella catarrhalis in adenoids, palatine tonsils, and nasopharyngeal washes was correlated with more severe tonsillar hypertrophy.

The liver fluke Fasciola hepatica (Trematoda: Fasciolidae) causes fascioliasis, which affects mostly domestic ruminants and humans worldwide. This parasite has an Old World origin and was introduced into the New World by European colonizers. Capybara ( Hydrochoerus hydrochaeris) is the largest living rodent species, with adults weighing over 60 kg. We report a fascioliasis outbreak caused by F. hepatica that reduced a capybara group from 21 to 2 animals within a 9-mo period. Animal infection and associated lesions were confirmed by postmortem examinations that revealed extensive liver damage associated with the presence of large number of adult and immature forms of F. hepatica. Both macroscopic and microscopic alterations in the liver were compatible with acute fascioliasis, which is characterized by a large parasite burden in the liver. Taxonomic identification of flukes collected from capybara livers were confirmed by molecular methods, which generated a mitochondrial NADH dehydrogenase I (NDI) gene partial sequence that was 100% identical to a F. hepatica NDI sequence from the United Kingdom. This is the first report of deleterious effects caused by F. hepatica in capybaras, highlighting the potential harm caused by this exotic parasite in the capybara.