Agroforestry of upland rice (Oryza sativa L.) and sengon tree (Paraserianthes falcataria) could increase the growth of sengon trees; however, it would also increase the percentage of empty grain due to shade from the tree. Fertilization with P is expected to increase plant height, grain weight and weight of straw of upland rice and growth of sengon tree. The aim of this research is to analyze the growth and production of sengon and upland rice with agroforestry and P fertilizer application. Application in cultivation of upland rice using split-split plot design. The main plot is agroforestry and monoculture, subplot of Sintanur and Situ Bagendit varieties and split-split plot of P fertilization consisting of 4 levels, namely: P 0 = 0 g / plant, P50 = 3 g / plant, P100 = 6 g / plant and P150 = 9 g / plant. The application of P fertilizer showed that P 100% had high production on Sintanur varieties with monoculture. Agroforestry system can increase the growth of sengon plants.Keywords: agroforestry, P fertilizer, sengon, upland rice.

Saninten (Castanopsis argentea (Blume) A.DC.) is one of the member Fagaceae family which can produce wood and non-wood product. Micropropagation of saninten by in vitro has never been reported. This research aims to identify the growth response of saninten shoot by adding Plant Growth Regulator (PGR), they are 6-benzylaminopurine (BAP) and indole-3-acetic-acid (IAA) with different concentrations in propagation in vitro on woody plant medium (WPM). The research conducted a completely randomized design (CRD) factorial. They are PGR type and PGR concentration. The PGR type consist of two levels namely BAP and IAA. PGR concentration consist of three levels namely 0 mg/L BAP and IAA, 0.5 mg/L BAP or 0.1 mg/L IAA, and 1.0 mg/L BAP or 0.1 mg/L IAA. Parameters observed is the amount of shoot, shoot height, and callus growth. The combination of BAP (0, 0.5, 1 mg/L) and IAA (0, 0.1, 0.1 mg/L) haven’t produce optimal growth of shoot. WPM medium with 0.5 mg/L BAP was able to produce the best of percentage of shoot, the number of shoots, and shoot height growth. WPM medium with IAA concentration of 0.1 and 0.2 mg/L produce explant with callus.Key words: 6-benzylaminopurine (BAP), Castanopsis argentea, indole-3-acetic-acid (IAA), woody plant medium (WPM)

Setyaningsih L, Wulandari AS, Hamim H. 2018. Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi. Biodiversitas 19: 454-459. Gold mine tailings contain extreme physical and chemical properties, which inhibit plant growth due to lower nutrition and higher heavy metal contaminants. Typha (Typha angustifolia) is type of grass growing well on waterlogged area including tailing dam. The objective of this study was to investigate the effect of arbuscular mycorrhizal fungi (AMF) in combination with compost and soil on the typha growth in gold mine tailings. The study was conducted in greenhouse by inoculating two AMF isolates (Glomus etunicatum and G. manihotis) to typha seedlings grown in pure tailing media, mixed tailing-compost media, and mixed tailing-compost-soil media. The compatibility and growth of typha grass were analysed after 1 month. Results showed that G. etunicatum and G. manihotis application significantly increased AMF colonization of typha roots up to 16.6% and 21.8% respectively. The length, number of leaves and biomass of typha also increased up to 90%, 50% and 97% respectively compared to those without AMF inoculation. G. etunicatum contributed the best growth of typha grown in mixed compost-soil-tailings, resulting in double increase of its length and biomass. The application of G. manihotis did not significantly increase the growth of typha in mixed media; however, under pure tailing, this mycorrhiza had the best induction for typha biomass and leaf number. In general, AMF application increased growth of typha grass in tailings media.

The aims of this research were to analyze the role of arbuscular mycorrhizal fungi (AMF) and humid acid toward balsa (Ochroma bicolor Rowlee.) growth on soil contaminated by lead (Pb) and to analyze Pb accumulation in balsa. This study used a split split plot design. The main plot was AMF with 5 levels; without AMF (A0), AMF from secondary forest (A1), AMF from rubber natural forest (A2), AMF from oil palm plantations (A3), and AMF from rubber plantations (A4). The subplot was humic acid with 2 levels; no humic acid (B0) and 100 mL humic acid (B1). The sub subplot was Pb with 3 levels; 0 ppm Pb (C0), 500 ppm Pb (C1), and 750 ppm Pb (C2). Observations of balsa seedlings was done until age of 22 weeks after planting in the greenhouse. Variables observed were height (cm), diameter (mm), shoot dry weight (g), root dry weight (g), FMA colonization (%), and the accumulation of Pb (ppm). The role of AMF and humic acid was more effective on the soil with 0 ppm of Pb than at 500 ppm of Pb and 750 ppm of Pb. AMF from rubber natural forest was the most effective AMF to increase diameter, root dry weight and shoot dry weight. Humic acid was able to increase the growth of height of 22.87% and diameter of 24.86% better than no humic acid. Pb accumulation in the entire plant tissue was more than 1000 ppm. It inhibited the growth of balsa seedlings and causing dead in 17.52% plant.

Cananga odorata (Lam.) Hook.f. & Thomson is one of the essential oils producing plants that have many benefits for humans. However, there is little information about the floral morphology and anatomy of C. odorata. The aim of this study is examining the floral morphology and anatomy of C. odorata. The flower morphology (color, shape, and size of the flower) were observed at fresh mature flowers. While, the flower anatomy (pistil, stamen, and ovary) were observed at flower histological samples. The flower was fixed, dehydrated, alcoholized, embedding in paraffin, and stained with safranin-fast green. The results of this study showed that flowers of C. odorata are complete flowers that had 3 green sepals (0.5 – 0.9 cm), 6 – 12 yellowish-green petals (2.5 – 7.2 cm), 8 – 15 dark green pistils (5 – 8 mm), and numerous stamens. The shape of pistils is oval and contains one ovary which has 8 – 12 ovules. The shape of the stamens is elongated oval and contains pollens. The information about the floral morphology and anatomy of C. odorata might enhance the understanding of future studies regarding natural reproduction and cultivation programs.

Abstract. Rahmawathi AM, Wijayanto N, Wulandari AS. 2017. Short Communication: Growth analysis of sentang (Azadirachta excelsa) in agroforestry system. Biodiversitas 18: 589-592. Sentang is one of forestry plants which can be planted in agroforestry system. It is one of the important medicinal plants grown in different agroforestry systems and contains an active compound of azadirachtin. It has cone crown and balance branch that makes it suitable to be cultivated in agroforestry system which utilizes lower stratum or ground. This research aimed to analyze growth and nutrient content of sentang in agroforestry and monoculture system. Plantation site was in Conservation Unit Research Center for Biopharmaceutical IPB. The sentang was two years old. The design was complete randomized design with one factor (cropping system) and two treatments (agroforestry and monoculture). The results showed the increase of sentang height was higher in agroforestry system than the monoculture due to additional nutrition obtained by sentang in agroforestry system derived from fertilizing activity at the beginning of soybean planting. Nutrient content of sentang was higher in agroforestry plot than monoculture. Fertilizing activity of soybean led to an increase of sentang in agroforestry system.

Abstract. Setyaningsih L, Dikdayatama FA, Wulandari AS. 2020. Arbuscular mycorrhizal fungi and Rhizobium enhance the growth of Samanea saman (trembesi) planted on gold-mine tailings in Pongkor, West Java, Indonesia. Biodiversitas 21: 611-616. Revegetation of severely degraded lands, such as gold-mine tailings, requires comprehensive approach including the selection of appropriate tree species and the improvement of soil fertility with the application of microorganisms. This study aimed to analyze the growth of trembesi (Samanea saman (Jacq.) Merr) seedlings inoculated with Arbuscular Mycorrhizal Fungi (AMF) and rhizobial bacteria (RB) on gold-mine tailings in Pongkor, Bogor, West Java, Indonesia. The AMF, RB, and mixture of AMF and RB were inoculated into S. saman seedlings and then the seedlings were grown on gold mine tailings land. Seedling growth, AMF infection and RB nodulation were observed after 12 weeks after planting. The results showed that the treatments facilitated AMF colonization by up to 20.7% and RB nodulation up to 22 nodules per plant. The inoculation of S. saman seedlings with AMF and RB significantly increased the growth on diameter, biomass, and root length, with values of root-to-shoot ratio of 3.1-4.3. The roots of S. saman seedlings were also able to penetrate the depth of the tailings solum. These results showed that AMF and RB application effectively increased the growth of S. saman seedlings in the gold tailings field.

Melaleuca alternifolia (tea tree) is one of the families of Myrtacea, which produces essential oils from their leaves, called tea tree oil (TTO) that is used as ingredients for cosmetics, antiseptics and pesticides. TTO leaves production of M. alternifolia plants can be increased by stimulating the growth of new shoots using 6-BAP hormone. The purpose of this study was to obtain appropriate dosage of 6-BAP to induce shoots in M. alternifolia seedlings. This research was conducted at the Dramaga Permanent Nursery in Bogor for 6 weeks from July-Agustus 2019 with M. alternifolia seedlings originating from grafts over 4 months old. Completely randomized design (CRD) with different 6-BAP concentration treatments in 5 replicates was used. The 6-BAP (benzyl amino purine) with a concentration of 5 ppm, 10 ppm, 15 ppm, 20 ppm and without hormone (as control) were sprayed on the leaves and stem. The results showed that 6-BAP affected shoot length and number of leaves. Spraying with 5 ppm 6-BAP dose increased the length of shoots and the number of leaves after 6 weeks of application amounting to 1.9% and 11.4%, respectively.