RESPON PERTUMBUHAN TUNAS SANINTEN (Castanopsis argentea (Blume) A.DC.) TERHADAP PEMBERIAN ZAT PENGATUR TUMBUH BAP DAN IAA SECARA IN VITRO The growth response of Saninten (Castanopsis argentea (Blume) A.DC.) In Vitro by Adding Plant Growth Regulator BAP

Wulandari, Arum Sekar; Sulistiani, Erina; Agustiani, Esthi Liani

doi:10.29244/j-siltrop.8.3.208-214

Cited by 8 publications

(11 citation statements)

References 2 publications

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“…tunas yang sama pada beberapa perlakuan yaitu 2 pereksplan. Menurut Wulandari et al (2017) menyatakan bahwa pemberian IAA dan BAP memiliki hasil mirip terhadap jumlah tunas dan persentase muncul tunas setiap perlakuan, yang diakibatkan belum tercapainya keseimbangan hormon auksin dan sitokinin yang terkandung pada media, selain itu jumlah tunas yag terbentuk juga bergantung pada kepekaan eksplan tanaman dalam menyerap hormon berbeda.…”

Section: Pembahasanunclassified

Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L)

Restanto,

Nafiah,

Fanata

et al. 2024

Al-Kauniyah J. Biol.

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AbstrakTanaman krisan varietas Suciono merupakan tanaman hias yang potensial dikembangkan dan diminati oleh penduduk dunia, yang ditunjukkan dengan permintaan pasar terhadap tanaman krisan meningkat sepanjang tahun. Hal ini menyebabkan perbanyakan krisan secara in vitro sangat penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh Indole Acetic Acid (IAA) dan Benzly Amino Purine (BAP) terhadap multiplikasi tunas krisan varietas Suciono. Desain penelitian ini menggunakan Rancangan Acak Lengkap dengan satu faktor yakni kombinasi hormon IAA dan BAP yang 9 kombinasi, yakni 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; dan 1 mg/L + 2,5 mg/L dimana setiap kombinasi diulang sebanyak 3 kali. Variabel yang diamati meliputi analisis histologis kalus, waktu muncul tunas, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil analisis ragam atau ANOVA menunjukkan bahwa kombinasi IAA 0 mg/L dan BAP 2 mg/L merupakan kombinasi konsentrasi terbaik untuk multiplikasi tunas. Penambahan BAP 2 mg/L mampu menghasilkan persentase muncul tunas. 100%, jumlah tunas 3 per-eksplan, tinggi tunas 7 cm, dan jumlah daun sebanyak 16 helai.AbstractThe variety Suciono of chrysanthemum is a potential ornamental plant that is in demand by the world's population, as indicated by the increasing market demand for chrysanthemum plants throughout the year. This has led to the importance of in vitro propagation of chrysanthemum. The aim of the this study is to determine the effect of Indole Acetic Acid (IAA) and Benzly Amino Purine (BAP) on the multiplication of chrysanthemum shoots of the Suciono variety. The design of this study used nodus explants, treated with a combination of IAA hormones and BAP. It is make 9 combinations, and then the combinations of hormones is 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; and 1 mg/L + 2,5 mg/L. The observed variables included histological analysis of callus, time of shoot emergence, number of shoots, shoots height, and number of leaves. The results of the analysis showed that the treatment with 0 mg/L IAA and 2 mg/L BAP was the best concentration for shoots multiplication. The addition of 2 mg/L BAP was able to produce a percentage of shoots emergence of 100%, 3 shoots per explant, shoots height of 7 cm and 16 leaves.

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Section: Pembahasanunclassified

Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L)

Restanto,

Nafiah,

Fanata

et al. 2024

Al-Kauniyah J. Biol.

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“…The research location was located in Materials and equipment Materials and tools needed for research include container, sprayer, cutting scissors, Erlenmeyer, measuring cup, pH meter, volumetric pipette, analytical balance, oven, autoclave, tweezers, stainless scissors, petri dish, blender, culture bottle, laminar air flow, culture rack, aluminum foil, plastic wrap, handsprayer, C. odorata forma Genuina from SEAMEO Biotrop, systemic fungicide (50.4% benomyl), systemic bactericidal (20% streptomycin sulfate), 96% ethanol, sterile distilled water, detergent, alcohol, tween-20, NaOCl, WPM media (Woody Plant medium), MS medium (Murashige and Skoog), agar, granulated sugar, BAP, NAA. The cleaned explants were then aseptically grown in WPM plus broad-spectrum antibiotic media in the LAF The culture bottles were then stored on shelves in the culture room at 22-25 °C air temperature and under one unit of 36-40 watt fluorescent lamps [10]. The design used for the sterilization stage was a completely randomized design.…”

Section: Research Locationmentioning

confidence: 99%

“…Data on the results of explants sterilization were tested with ANOVA; if the analysis results were significantly different, and then continued with the Duncan's advanced test [17]. Callus scoring data were analyzed using Kruskal-Wallis analysis [10]. Data was processed using Minitab, Microsoft Excel, and SAS version 9.1.…”

Section: Research Locationmentioning

confidence: 99%

In vitro sterilization and callus induction from Cananga odorata forma genuina steenis explants

Nugraheni¹,

Mansur

Sulistiani

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Cananga odorata (ylang-ylang) is a plant with numerous uses, including ornamental use, traditional medicinal ingredient, as fragrance, and cosmetics. The high market demand for ylang-ylang essential oil needs to be supported by information on the in vitro process of callus formation to supports the secondary metabolites production without having to cut or harvest the trees so that biodiversity is maintained. The aims of this study were to analyze the explants sterilization method using sodium hypochlorite (NaOCl) solution that was most suitable for the ylang-ylang initiation, and to analyze the effect of BAP and NAA treatment on the formation of ylang-ylang callus. The design used is a completely randomized design. The methods used included the preparation, sterilization, and callus induction using a combination of BAP and NAA hormones with twenty four treatment combinations. The results are: the sterilization of explants with sodium hypochlorite (NaOCl) solution at a concentration of 20% (v/v) 10 minutes followed by a concentration of 15% (v/v) 20 minutes was able to produce sterile explants in as much as 67.5% of cases. At the callus induction stage, the highest score of callus formation in leaf explants was in media-23 (BAP 4 ppm plus NAA 5 ppm).

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“…Akan tetapi saat ini jumlah C. argentea (Blume) A.DC. semakin menurun karena tingkat pertumbuhan yang lama, adanya pembalakan dalam pengambilan kayunya dan permudaan alami yang sedikit (Wulandari, Arum Sekar, 2017). Banyaknya manfaat tanaman ini menjadikan tanaman ini harus dilakukan perbanyakan agar tidak punah.…”

Section: Pendahuluanunclassified

“…Penelitian (Wulandari, Arum Sekar, 2017) menyatakan bahwa terjadinya kontaminasi pada eksplan yang telah dilakukan proses sterilisasi diduga dikarenakan proses sterilisasi yang kurang optimal. Menurut (Zulkarnain, 2009), kontaminasi mikroorganisme yang terjadi berasal dari berbagai faktor diantaranya alat yang digunakan pada proses sterilisasi tidak sempurna, tempat kerja yang masih kurang steril, berasal dari eksplan ataupun proses pengerjaan yang kurang teliti.…”

Section: Hasil Dan Pembahasanunclassified

Explant Sterilization Technique Castanopsis argentea (Blume) A.DC. with the addition of Ascorbic Acid and Sodium Hypochlorite (NaOCl) In Vitro

Chika

Ismaini

Armanda

2022

bib

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Castanopsis argentea (Blume) A.DC. has the local name saninten. This plant is one of the woody plants that has the potential to be developed because it is beneficial for wildlife to nest and is used in reforestation. The seeds of this plant can be used for food by boiling and burning. This plant must be preserved so that it does not become extinct. However, the propagation, so it must use the technique of propagation through tissue culture. This study aims to determine the best sterilization technique for in vitro culture of C. argentea. Eight methods of explant sterilization have been carried out in this study. Methods 1-4 do not use ascorbic acid immersion, while methods 5-8 use the addition of ascorbic acid. Based on observations, it is known that the best sterilization is in the 8th method because the level of contamination and browning is lower than other methods, namely on the fifth day after planting only 20%. The 8th method is graded sterilization. In graded sterilization, explants were gradually immersed in 10% NaOCl, 20% NaOCl, and 30% NaOCl, respectively. Multilevel sterilization is the most effective method of sterilization to reduce contamination and browning. Ascorbic acid acts as an antioxidant and reduces contamination in the enzymatic browning reaction. The combination of sterile substances, time, sequence, and method of immersion affects the sterility of explants.

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RESPON PERTUMBUHAN TUNAS SANINTEN (Castanopsis argentea (Blume) A.DC.) TERHADAP PEMBERIAN ZAT PENGATUR TUMBUH BAP DAN IAA SECARA IN VITRO The growth response of Saninten (Castanopsis argentea (Blume) A.DC.) In Vitro by Adding Plant Growth Regulator BAP

Cited by 8 publications

References 2 publications

Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L)

Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L)

In vitro sterilization and callus induction from Cananga odorata forma genuina steenis explants

Explant Sterilization Technique Castanopsis argentea (Blume) A.DC. with the addition of Ascorbic Acid and Sodium Hypochlorite (NaOCl) In Vitro

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