Agroforestry of upland rice (Oryza sativa L.) and sengon tree (Paraserianthes falcataria) could increase the growth of sengon trees; however, it would also increase the percentage of empty grain due to shade from the tree. Fertilization with P is expected to increase plant height, grain weight and weight of straw of upland rice and growth of sengon tree. The aim of this research is to analyze the growth and production of sengon and upland rice with agroforestry and P fertilizer application. Application in cultivation of upland rice using split-split plot design. The main plot is agroforestry and monoculture, subplot of Sintanur and Situ Bagendit varieties and split-split plot of P fertilization consisting of 4 levels, namely: P 0 = 0 g / plant, P50 = 3 g / plant, P100 = 6 g / plant and P150 = 9 g / plant. The application of P fertilizer showed that P 100% had high production on Sintanur varieties with monoculture. Agroforestry system can increase the growth of sengon plants.Keywords: agroforestry, P fertilizer, sengon, upland rice.

Saninten (Castanopsis argentea (Blume) A.DC.) is one of the member Fagaceae family which can produce wood and non-wood product. Micropropagation of saninten by in vitro has never been reported. This research aims to identify the growth response of saninten shoot by adding Plant Growth Regulator (PGR), they are 6-benzylaminopurine (BAP) and indole-3-acetic-acid (IAA) with different concentrations in propagation in vitro on woody plant medium (WPM). The research conducted a completely randomized design (CRD) factorial. They are PGR type and PGR concentration. The PGR type consist of two levels namely BAP and IAA. PGR concentration consist of three levels namely 0 mg/L BAP and IAA, 0.5 mg/L BAP or 0.1 mg/L IAA, and 1.0 mg/L BAP or 0.1 mg/L IAA. Parameters observed is the amount of shoot, shoot height, and callus growth. The combination of BAP (0, 0.5, 1 mg/L) and IAA (0, 0.1, 0.1 mg/L) haven’t produce optimal growth of shoot. WPM medium with 0.5 mg/L BAP was able to produce the best of percentage of shoot, the number of shoots, and shoot height growth. WPM medium with IAA concentration of 0.1 and 0.2 mg/L produce explant with callus.Key words: 6-benzylaminopurine (BAP), Castanopsis argentea, indole-3-acetic-acid (IAA), woody plant medium (WPM)

Setyaningsih L, Wulandari AS, Hamim H. 2018. Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi. Biodiversitas 19: 454-459. Gold mine tailings contain extreme physical and chemical properties, which inhibit plant growth due to lower nutrition and higher heavy metal contaminants. Typha (Typha angustifolia) is type of grass growing well on waterlogged area including tailing dam. The objective of this study was to investigate the effect of arbuscular mycorrhizal fungi (AMF) in combination with compost and soil on the typha growth in gold mine tailings. The study was conducted in greenhouse by inoculating two AMF isolates (Glomus etunicatum and G. manihotis) to typha seedlings grown in pure tailing media, mixed tailing-compost media, and mixed tailing-compost-soil media. The compatibility and growth of typha grass were analysed after 1 month. Results showed that G. etunicatum and G. manihotis application significantly increased AMF colonization of typha roots up to 16.6% and 21.8% respectively. The length, number of leaves and biomass of typha also increased up to 90%, 50% and 97% respectively compared to those without AMF inoculation. G. etunicatum contributed the best growth of typha grown in mixed compost-soil-tailings, resulting in double increase of its length and biomass. The application of G. manihotis did not significantly increase the growth of typha in mixed media; however, under pure tailing, this mycorrhiza had the best induction for typha biomass and leaf number. In general, AMF application increased growth of typha grass in tailings media.

The aims of this research were to analyze the role of arbuscular mycorrhizal fungi (AMF) and humid acid toward balsa (Ochroma bicolor Rowlee.) growth on soil contaminated by lead (Pb) and to analyze Pb accumulation in balsa. This study used a split split plot design. The main plot was AMF with 5 levels; without AMF (A0), AMF from secondary forest (A1), AMF from rubber natural forest (A2), AMF from oil palm plantations (A3), and AMF from rubber plantations (A4). The subplot was humic acid with 2 levels; no humic acid (B0) and 100 mL humic acid (B1). The sub subplot was Pb with 3 levels; 0 ppm Pb (C0), 500 ppm Pb (C1), and 750 ppm Pb (C2). Observations of balsa seedlings was done until age of 22 weeks after planting in the greenhouse. Variables observed were height (cm), diameter (mm), shoot dry weight (g), root dry weight (g), FMA colonization (%), and the accumulation of Pb (ppm). The role of AMF and humic acid was more effective on the soil with 0 ppm of Pb than at 500 ppm of Pb and 750 ppm of Pb. AMF from rubber natural forest was the most effective AMF to increase diameter, root dry weight and shoot dry weight. Humic acid was able to increase the growth of height of 22.87% and diameter of 24.86% better than no humic acid. Pb accumulation in the entire plant tissue was more than 1000 ppm. It inhibited the growth of balsa seedlings and causing dead in 17.52% plant.

Cananga odorata (Lam.) Hook.f. & Thomson is one of the essential oils producing plants that have many benefits for humans. However, there is little information about the floral morphology and anatomy of C. odorata. The aim of this study is examining the floral morphology and anatomy of C. odorata. The flower morphology (color, shape, and size of the flower) were observed at fresh mature flowers. While, the flower anatomy (pistil, stamen, and ovary) were observed at flower histological samples. The flower was fixed, dehydrated, alcoholized, embedding in paraffin, and stained with safranin-fast green. The results of this study showed that flowers of C. odorata are complete flowers that had 3 green sepals (0.5 – 0.9 cm), 6 – 12 yellowish-green petals (2.5 – 7.2 cm), 8 – 15 dark green pistils (5 – 8 mm), and numerous stamens. The shape of pistils is oval and contains one ovary which has 8 – 12 ovules. The shape of the stamens is elongated oval and contains pollens. The information about the floral morphology and anatomy of C. odorata might enhance the understanding of future studies regarding natural reproduction and cultivation programs.