Hydra feeding response is a very primitive olfactory-like behavior present in a multicellular organism. We investigated the role of nitric oxide (NO) in the induction and control of hydra feeding response. Under basal conditions, hydra specimens produce detectable amounts of nitrite (NO2-), the breakdown product of NO. When hydra were incubated with reduced glutathione (GSH), the typical activator of feeding response, an increase of basal NO production was observed. This effect was inhibited by glutamic or alpha-aminoadipic acids, two GSH antagonists, which block GSH-induced feeding response, and by the NO synthase (NOS) inhibitor L-NAME. Moreover, we found that hydra possess a calcium-dependent (but calmodulin-independent) NOS isoform. By using exogenous NO donors and NOS inhibitors, we demonstrated that NO stimulus can participate both in triggering tentacular movements and in recruiting neighbor tentacles during hydra feeding response. By using dbt2-cGMP, an analog to cGMP, we observed that the NO effect was independent of cGMP pathway. Our results strongly implicate NO involvement in hydra very primitive feeding behavior, thus confirming its preservation throughout evolution.
Over the last four centuries several botanists have analysed the Colosseum flora, and by using modern ecological and biogeographical approaches to these same floras, we have observed similar trends with respect to the climatic phenomena as those of the historic Torre Calandrelli Observatory and by other older historical records. The elements of which can be summarised as follows: 1) a substantial reduction of all the biogeographical components taken as indicators of cooler and damper habitats, such as the Euro-Asiatic and Boreal areas starting from the 17th Century in particular; 2) a decrease in numbers of all the species more dependent on water (independent of their chorology); 3) similar clear-cut increases in the species belonging to phytosociological classes typical to Mediterranean climatic conditions, to the detriment of those typical to Middle-European temperate bioclimates. All of which can be interpreted as clear indicators of non-negligible climatic changes towards hotter and drier conditions independent of local variations due to urbanisation.
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