A wide sampling for isolating highly destructive and extremotolerant black fungi was performed from Italian monuments in selected historical sites which include the “Cortile della Pigna”, Vatican Museum and the St Peter colonnade (Vatican City State), the monumental cemetery of Bonaria (Cagliari), and other monuments in the city of Cagliari. Thirty out of seventy-four strains isolated were identified basing on ITS Blastn comparison. Based on multilocus phylogeny and morphological data, one new genus and species Lithophila guttulata, five new species Knufia marmoricola, K. vaticanii, K. karalitana, K. mediterranea and Exophiala bonariae, order Chaetothyriales and one new genus and species, Saxophila tyrrhenica, and two new species Vermiconia calcicola and Devriesia sardiniae, order Capnodiales, were proposed. Ecological considerations are put forward

The island of Bali has several traditional Aga villages that survive under the pressures of an intense tourist industry and agricultural changes. In order to understand possible impacts on traditional ethnobotanical knowledge (TEK) in Bali, we interviewed local people living in 13 traditional villages regarding the number of known plants and their uses. We analyzed socioeconomic factors influencing change of such knowledge at both individual (informant) and community (village) level. We identified a total of 149 food and nutraceutical plants being used in the study area. Neither gender, occupation, income, nor level of formal education had a significant effect on TEK. However, informant’s age and village status were found to play an important role in the retention of TEK at an individual level. At the village level, the use of Internet/smart phones was an important predictor of cultural erosion

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SummaryThe inventory of microorganisms responsible for biological deterioration of ancient paintings has become an integral part of restoration activities. Here, the microbial agent of rosy discoloration on medieval frescoes in the Crypt of the Original Sin (Matera, Italy) was investigated by a combination of microscopic, molecular and spectroscopic approaches. The bacterial community from three rosy-discoloured painting sites was characterized by 16S rRNA gene-based techniques. The eubacterial population was prevalently composed of Actinobacteria, among which Rubrobacter radiotolerans-related bacteria accounted for 63-87% of the 16S rRNA gene pool per sampled site. Archaea, with prevalence of Haloarchaea-related species, were detected in one of the three sites where they accounted for < 0.1% of the total 16S rRNA gene pool. Raman spectroscopy confirmed the identity between R. radiotolerans carotenoids (bacterioruberins) and pigments responsible for colour alteration of frescoes. This investigation provides the first evidence of a causal relationship between heavy contamination by Rubrobacter-related bacterioruberinproducing bacteria and rosy discoloration of ancient wall paintings.

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